A5046290894- Cancer Cells and Metastasis
- Cancer-related gene regulation
- RNA modifications and cancer
- Ovarian cancer diagnosis and treatment
- Epigenetics and DNA Methylation
- Autophagy in Disease and Therapy
- Chemical Synthesis and Analysis
- MicroRNA in disease regulation
- PARP inhibition in cancer therapy
- HER2/EGFR in Cancer Research
- Extracellular vesicles in disease
- Cancer-related molecular mechanisms research
- Cell Adhesion Molecules Research
- Nuclear Structure and Function
- Angiogenesis and VEGF in Cancer
- FOXO transcription factor regulation
- Cancer Mechanisms and Therapy
- Cancer Research and Treatments
- Reproductive Biology and Fertility
- Caveolin-1 and cellular processes
- RNA Interference and Gene Delivery
- Wnt/β-catenin signaling in development and cancer
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Photosynthetic Processes and Mechanisms
The University of Texas MD Anderson Cancer Center
2016-2025
Oil Crops Research Institute
2021-2023
Chinese Academy of Agricultural Sciences
2021-2023
Ministry of Agriculture and Rural Affairs
2021-2023
Sanford Burnham Prebys Medical Discovery Institute
2023
Chongqing University of Posts and Telecommunications
2023
Rice University
2023
George Washington University
2023
Peking Union Medical College Hospital
2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2023
Thrombocytosis is present in more than 30% of patients with solid malignancies and correlates worsened patient survival. Tumor cell interaction various cellular components the tumor microenvironment including platelets crucial for growth metastasis. Although it known that can infiltrate into tissue, secrete pro-angiogenic pro-tumorigenic factors thereby increase growth, precise molecular interactions between metastatic cancer cells are not well understood. Here we demonstrate induce...
We previously found focal adhesion kinase (FAK) inhibition sensitizes ovarian cancer to taxanes; however, the mechanisms are not well understood.We characterized biologic response of taxane-resistant and taxane-sensitive models a novel FAK inhibitor (VS-6063). used reverse-phase protein arrays (RPPA) identify downstream targets in cell lines. Furthermore, we correlated clinical pathological data with nuclear cytoplasmic expression YB-1 105 samples. Statistical tests were two-sided, P values...
// Sanchaika Gaur 1,2,9 , Yunfei Wen 3 Jian H. Song 1 Nila U. Parikh Lingegowda S. Mangala 3,4 Alicia M. Blessing 5 Cristina Ivan Sherry Y. Wu Andreas Varkaris Yan Shi Gabriel Lopez-Berestein 4,6 Daniel E. Frigo 5,7 Anil K. Sood 2,3,4,8 and Gary Gallick 1,2 Department of Genitourinary Medical Oncology, David Koch Center for Applied Research Cancers, The University Texas, MD Anderson Cancer Center, Houston, TX, USA 2 Program in Biology Metastasis, Texas Graduate School Biomedical Sciences at...
Photoacoustic (PA) imaging is a functional and molecular technique capable of high sensitivity spatiotemporal resolution at depth. Widespread use PA imaging, however, limited by currently available contrast agents, which either lack PA-signal-generation ability for deep or their absorbance spectra overlap with hemoglobin, reducing sensitivity. Here we report on agent based targeted liposomes loaded J-aggregated indocyanine green (ICG) dye (i.e., PAtrace) that synthesized, bioconjugated,...
Abstract Antiangiogenic treatment targeting the vascular endothelial growth factor (VEGF) pathway is a powerful tool to combat tumor and progression; however, drug resistance frequently emerges. We identify CD5L (CD5 antigen-like precursor) as an important gene upregulated in response antiangiogenic therapy leading emergence of adaptive resistance. By using both RNA-aptamer monoclonal antibody CD5L, we are able abate pro-angiogenic effects overexpression vitro vivo settings. In addition,...
Abstract Small RNAs (microRNAs [miRNAs] or small interfering [siRNAs]) are effective tools for cancer therapy, but many of the existing carriers their delivery limited by low bioavailability, insufficient loading, impaired transport across biological barriers, and into tumor microenvironment. Extracellular vesicle (EV)–based communication in mammalian plant systems is important physiological pathological processes, EVs show promise as RNA interference molecules. However, some fundamental...
// Yinghong Pan 1, * , Gordon Robertson 2, Lykke Pedersen 3 Emilia Lim 2 Anadulce Hernandez-Herrera 1 Amy C. Rowat 4 Sagar L. Patil Clara K. Chan Yunfei Wen 5 Xinna Zhang 5, 6 Upal Basu-Roy 9 Alka Mansukhani Andy Chu Payal Sipahimalani Reanne Bowlby Denise Brooks Nina Thiessen Cristian Coarfa 14 Yussanne Ma Richard A. Moore Jacquie E. Schein Andrew J. Mungall Jinsong Liu 8 Chad V. Pecot 10 Anil Sood 6, 7, Steven J.M. Jones 12, 13 Marco Marra Preethi H. Gunaratne 11 Department of Biochemistry...
Abstract Purpose: Cancer cells are highly dependent on folate metabolism, making them susceptible to drugs that inhibit receptor activities. Targeting overexpressed alpha (FRα) in cancer offers a therapeutic opportunity. We investigated the functional mechanisms of MORAB-003 (farletuzumab), humanized mAb against FRα, ovarian models. Experimental Design: first examined FRα expression an array human cell lines and then assessed vivo effect tumor growth progression several orthotopic mouse...
Abstract Introduction: EphB4 is frequently overexpressed in ovarian and other solid tumors involved interactions between tumor cells microenvironment, contributing to metastasis. its membrane-bound ligand ephrin B2 (EFNB2) mediates bi-directional signaling. Currently, no small-molecule-based, peptides with dual functional binding are available. Therefore, we developed an agonist that capable of activating the forward signaling interfering reverse EFNB2 Methods: A high-affinity peptide...
Antibodies targeting folate receptor 1 (FOLR1) induce autophagic cell death in addition to antibody-dependent cytotoxicity, but the biological relevance of anti-FOLR1 antibody-induced autophagy for clinical applications remains unclear. Here, we investigated role triggered by IMGN853 (mirvetuximab soravtansine), a FOLR1-targeted antibody-drug conjugate, and explored potential combinations with chemotherapeutic drugs used ovarian cancer treatment. We discovered that FOLR1 was predominantly...
<p>Time-lapse videos of GFP and RFP fluorescence in ptLC3-pEGFP-RFP–expressing OVCAR-8 cells during treatment with IMGN853 (Movie 2)</p>
<p>IMGN853 induces beclin-1–dependent autophagic cell death in FOLR1<sup>+</sup> HGSC lines. <b>A,</b> Percentages of vacuoles OVCAR-8 and OVCA432 cells FOLR1<sup>low</sup>A2780 treated with control (formulation buffer), IMGN853 alone, HCQ or IMGN853+HCQ for 48 hours analyzed by acridine orange staining followed FACS. <i>n</i> = 6; error bars represent SEMs. <b>B,</b> SYTOX<sup>+</sup> dead among...
<p>Combination therapy with IMGN853 and the anti–VEGF-A antibody B20 in OVCAR-8 model. <b>A,</b> Schematic representation of mouse models established using ip2-luc cells (4 × 10<sup>6</sup>/mouse). <b>B,</b> Twenty-one days after cell injection as shown by bioluminescence signals representative mice from IVIS imaging, were randomized to receive buffer (control), alone (5.2 mg/kg, once weekly), (5 twice weekly, intraperitoneally), or IMGN853+B20....
<p>Time-lapse videos of GFP and RFP fluorescence in ptLC3-pEGFP-RFP–expressing OVCAR-8 cells during treatment with the control formulation buffer (Movie 1)</p>
<p>Effects of IMGN853 on the survival and inhibition tumor growth mice bearing ovarian high-grade serous carcinoma (HGSC) PDX tumors. <b>A,</b> Main clinical pathologic characteristics tissues from patients with HGSC. Date Implantation (DATE) <b>B,</b> Schematic representation experiments. mouse models were established by surgical implantation HGSC tumors via intraovarian injection. Treatment either formulation buffer (control) or (5.2 mg/kg, once weekly) was...
<p>Combination therapy with IMGN853 and topotecan in the OVCAR-8 model. <b>A,</b> Results of MTT assays showing combinatorial effects different concentrations on viability cells after 72 hours treatment [shown as log IMGN853(M)]. <i>n</i> = 3; data are mean ± SD. <b>B,</b> Synergy quantification drug combination using Chou–Talalay analysis was reflected by CI plot for topotecan. <b>C,</b> Schematic representation mouse models ovarian...
<p>IMGN853 treatment increases autophagic flux and cell death in FOLR1<sup>+</sup> ovarian high-grade serous carcinoma (HGSC) models. <b>A,</b> Representative confocal IF images showing expression patterns of the autophagy markers LC3B (green) beclin-1 control- (formulation buffer, top row) IMGN853-treated PDX tumors (bottom row). <b>B,</b> Fluorescence intensity each image was quantified using Imaris v. 9.1.2. The sum fluorescence for marker is...
<p>Combination therapy with IMGN853 and the PARP inhibitor olaparib in OVCAR-8 model. <b>A,</b> MTT assay results showing combinatorial effects of different concentrations IMGN853+olaparib on viability cells after 72 hours treatment [shown as log IMGN853(M)]. <i>n</i> = 3; data are mean ± SD. <b>B,</b> The CI plot for olaparib. <b>C,</b> Schematic representation mouse models established using ip2-luc (4 ×...
<p>Ovarian high-grade serous carcinomas (HGSCs) have heterogeneous FOLR1 expression. <b>A</b> and <b>B,</b> UMAP visualization of major cell types in five freshly resected tumors patients with HGSC [three primary (T) two omental metastases (OMT)] by sample type (<b>A</b>) (<b>B</b>). <b>C</b> <b>D,</b> expression epithelial cells after reclustering the level <b>E,</b> Representative IHC images tissue...
<p>Supplementary Figures S1 to S8</p>