A5018158757- Single-cell and spatial transcriptomics
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Advanced Biosensing Techniques and Applications
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Gene expression and cancer classification
- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- Brain Metastases and Treatment
- Peptidase Inhibition and Analysis
- Protein Degradation and Inhibitors
- Ferroptosis and cancer prognosis
- Immune cells in cancer
- Ovarian cancer diagnosis and treatment
- Extracellular vesicles in disease
- Bioinformatics and Genomic Networks
- interferon and immune responses
- Phagocytosis and Immune Regulation
- Histone Deacetylase Inhibitors Research
- Cancer Research and Treatments
- Protease and Inhibitor Mechanisms
- Computational Drug Discovery Methods
- Ubiquitin and proteasome pathways
Houston Methodist
2016-2025
Cornell University
2014-2024
Weill Cornell Medicine
2018-2024
Methodist Hospital
2014-2024
Methodist Hospital
2013-2023
Bioengineering Center
2017
University of Utah
2014
Huntsman Cancer Institute
2014
Dana-Farber Cancer Institute
2014
Peking University
2014
Thrombocytosis is present in more than 30% of patients with solid malignancies and correlates worsened patient survival. Tumor cell interaction various cellular components the tumor microenvironment including platelets crucial for growth metastasis. Although it known that can infiltrate into tissue, secrete pro-angiogenic pro-tumorigenic factors thereby increase growth, precise molecular interactions between metastatic cancer cells are not well understood. Here we demonstrate induce...
Advanced high-grade serous ovarian cancer (HGSC) is an aggressive disease that accounts for 70% of all deaths. Nevertheless, 15% patients diagnosed with advanced HGSC survive more than 10 years. The elucidation predictive markers these long-term survivors (LTS) could help identify therapeutic targets the disease, and thus improve patient survival rates. To investigate stromal heterogeneity tumor microenvironment (TME) in cancer, we used spatial transcriptomics to generate spatially resolved...
Human pluripotent stem cells (hPSCs), including human embryonic and induced cells, are unique cell sources for disease modeling, drug discovery screens, therapy applications. The first step in producing neural lineages from hPSCs is the generation of (NSCs). Current methods NSC derivation involve time-consuming, labor-intensive steps an embryoid body or coculture with stromal lines that result low-efficiency NSCs. In this study, we report a highly efficient serum-free induction medium can...
The bromodomain and extra-terminal (BET) protein family members, including BRD4, bind to acetylated lysines on histones regulate the expression of important oncogenes, for example, c-MYC BCL2. Here, we demonstrate sensitizing effects histone hyperacetylation-inducing pan-histone deacetylase (HDAC) inhibitor panobinostat human acute myelogenous leukemia (AML) blast progenitor cells (BPC) BET antagonist JQ1. Treatment with JQ1, but not its inactive enantiomer (R-JQ1), was highly lethal against...
Abstract Motivation: Currently there are no curative anticancer drugs, and drug resistance is often acquired after treatment. One of the reasons that cancers complex diseases, regulated by multiple signaling pathways cross talks among pathways. It expected combinations can reduce improve patients’ outcomes. In clinical practice, ideal feasible existing Food Drug Administration-approved drugs or bioactive compounds already used on patients have entered trials passed safety tests. These could...
Emerging studies have begun to demonstrate that reprogrammed stromal cells play pivotal roles in tumor growth, metastasis, and resistance therapy. However, the contribution of non-small-cell lung cancer (NSCLC) has remained underexplored. We used an orthotopic model Kras-driven NSCLC systematically dissect specific hematopoietic cancer. RNA deep-sequencing analysis individually sorted myeloid lineage epithelial revealed cell-type-specific differentially regulated genes, indicative activated...
The molecular mechanism by which cancer-associated fibroblasts (CAFs) confer chemoresistance in ovarian cancer is poorly understood. purpose of the present study was to evaluate roles CAFs modulating tumor vasculature, chemoresistance, and disease progression. Here, we found that upregulated lipoma-preferred partner (LPP) gene microvascular endothelial cells (MECs) LPP expression levels intratumoral MECs correlated with survival patients cancer. Mechanistically, increased focal adhesion...
Purpose: Recent studies indicated that dysregulation of noncoding RNAs (ncRNA) such as miRNAs is involved in pathogenesis various human cancers. However, the molecular mechanisms underlying miR-34a are not fully understood triple-negative breast cancer (TNBC).Experimental Design: We performed vitro functional assays on TNBC cell lines to investigate role FOXM1/eEF2K signaling axis. tumor xenograft models were used for vivo therapeutic delivery miR-34a.Results: In this study, we investigated...
Triple-negative breast cancer (TNBC) exhibits more traits possessed by stem cells (CSC) than other subtypes and is likely to develop brain metastases. TNBC patients usually have shorter survival time after diagnosis of metastasis, suggesting an innate ability tumor in adapting the brain. In this study, we establish novel animal models investigate early adaptation metastases introducing both patient-derived cell line-derived CSC-enriched metastasis tumorsphere into mice. We discovered...
Cancer patients often show heterogeneous drug responses such that only a small subset of is sensitive to given anticancer drug. With the availability large-scale genomic profiling via next-generation sequencing, it now economically feasible profile whole transcriptome and genome individual in order identify their unique genetic mutations differentially expressed genes, which are believed be responsible for responses. Although subtyping analysis has identified patient subgroups sharing common...
Recent studies demonstrate the role of tumor microenvironment in progression. However, strategies used to overcome malignant phenotypes cancer cells modulated by have not been thoroughly explored. In this study, we evaluated therapeutic efficacy a newly developed mAb targeting microfibril-associated protein 5 (MFAP5), which is secreted predominately cancer-associated fibroblast (CAF), ovarian and pancreatic models.Experimental Design: MAbs were using human MFAP5 recombinant as an antigen...
// Xiaoping Zhu 1, * , Jing Zhong 2, Zhen Zhao 3 Jianting Sheng 1 Jiang Wang 4 Jiyong Liu 5 Kemi Cui Jenny Chang 6 Hong Stephen Wong Department of Systems Medicine and Bioengineering, Houston Methodist Research Institute, Weill Cornell Medical College, Houston, TX, USA 2 Radiology, The Teaching Hospital Fujian University, Provincial Cancer Hospital, Fuzhou, China Zhongda Nanjing, Orthopedics, Tongji Wuhan, Pharmacy, Changhai Shanghai, Center, These authors have contributed equally to this...
Here, we show that HEMATOLOGICAL AND NEUROLOGICAL EXPRESSED 1-LIKE (HN1L) is a targetable breast cancer stem cell (BCSC) gene altered in 25% of whole and significantly correlated with shorter overall or relapse-free survival triple-negative (TNBC) patients. HN1L silencing reduced the population BCSCs, inhibited tumor initiation, resensitized chemoresistant tumors to docetaxel, hindered progression multiple TNBC line-derived xenografts. Additionally, signatures associated disease-free We...
Abstract Advanced ovarian cancer usually spreads to the omentum. However, omental cell-derived molecular determinants modulating its progression have not been thoroughly characterized. Here, we show that circulating ITLN1 has prognostic significance in patients with advanced cancer. Further studies demonstrate suppresses lactotransferrin’s effect on cell invasion potential and proliferation by decreasing MMP1 expression inducing a metabolic shift metastatic cells. Additionally,...
Spatial statistics are crucial for analyzing clustering patterns in various spaces, such as the distribution of trees a forest or stars sky. Advances spatial biology, single-cell transcriptomics, enable researchers to map gene expression within tissues, offering unprecedented insights into cellular functions and disease pathology. Common methods deriving relationships include density-based (quadrat analysis, kernel density estimators) distance-based (nearest-neighbor distance [NND], Ripley's...
Bulk tumor tissue samples are used for generating gene expression profiles in most research studies, making it difficult to decipher the stroma–cancer crosstalk networks. In present study, we describe use of microdissected transcriptome identification cancer–stroma networks with prognostic value, which presents a unique opportunity developing new treatment strategies ovarian cancer. Transcriptome from cancer–associated fibroblasts (CAFs) and cancer cells patients high-grade serous (n = 70)...
KRAS is one of the most frequently mutated proto-oncogenes in pancreatic ductal adenocarcinoma (PDAC) and aberrantly activated triple-negative breast cancer (TNBC). A profound role microRNAs (miRNAs) pathogenesis human being uncovered, including therapy. Using silico prediction algorithms, we identified miR-873 as a potential regulator KRAS, investigated its PDAC TNBC. We found that reduced expression associated with shorter patient survival both cancers. significantly repressed TNBC cell...
Stromal and immune cells in the tumor microenvironment (TME) have been shown to directly affect high-grade serous ovarian cancer (HGSC) malignant phenotypes, however, how these interact influence HGSC patients’ survival remains largely unknown. To investigate cell-cell communication such a complex TME, we developed SpatioImageOmics (SIO) pipeline that combines imaging mass cytometry (IMC), location-specific transcriptomics, deep learning identify distribution of various stromal, as well...
Spatially resolved transcriptomics has revolutionized genome-scale transcriptomic profiling by providing high-resolution characterization of transcriptional patterns. Here, we present our spatial analysis framework, MUSTANG (MUlti-sample Spatial Transcriptomics data ANalysis with cross-sample similarity Guidance), which is capable performing multi-sample spot cellular deconvolution allowing both expression-based information sharing as well correlation in gene expression patterns within...
Epithelial-to-mesenchymal transition (EMT) contributes significantly to chemotherapy resistance and remains a critical challenge in treating advanced breast cancer. The complexity of EMT, involving redundant pro-EMT signaling pathways its paradox reversal process, mesenchymal-to-epithelial (MET), has hindered the development effective treatments. In this study, we utilized Tri-PyMT EMT lineage-tracing model mice single-cell RNA sequencing (scRNA-seq) comprehensively analyze status tumor...
Personalized genomics instability, e.g., somatic mutations, is believed to contribute the heterogeneous drug responses in patient cohorts. However, it difficult discover personalized driver mutations that are predictive of sensitivity owing diverse and complex individual patients. To circumvent this problem, a novel computational method presented potential relevant cancer subtypes identify mutation modules by coupling differentially expressed genes (DEGs) based subtyping analysis with...