Abstract Background Chinese indigenous sheep are valuable resources with unique features and characteristics. They distributed across regions different climates in mainland China; however, few reports have analyzed the environmental adaptability of based on their genome. We examined variants signatures selection involved adaptation to extreme humidity, altitude, temperature conditions 173 genomes from 41 phenotypically geographically representative breeds characterize genetic basis...

Body weight is an important economic trait for sheep and it vital their successful production breeding. Therefore, identifying the genomic regions biological pathways that contribute to understanding variability in body traits significant selection purposes. In this study, genome-wide associations of birth, weaning, yearling, adult weights 460 fine-wool were determined using resequencing technology. The results showed 113 single nucleotide polymorphisms (SNPs) reached significance levels...

Abstract Background The quality and yield of wool determine the economic value fine-wool sheep. Therefore, discovering markers or genes relevant to traits is cornerstone for breeding In this study, we used Illumina HiSeq X Ten platform re-sequence 460 sheep belonging four different breeds, namely, Alpine Merino (AMS), Chinese (CMS), Aohan (AHS) Qinghai (QHS). Eight traits, including fiber diameter (FD), coefficient variance (FDCV), standard deviation (FDSD), staple length (SL), greasy fleece...

Dongxiang tribute sheep have a history of use in food dishes such as "Dongxiang Handgrip," which dates back hundreds years and is favorite halal northwestern China. However, little known about the mutton quality characteristics sheep. Here, we measured sensory characteristics, nutritional quality, flavor substances to comprehensively evaluate these The qualities tribute, Tibetan, Ujumqin, Hu were evaluated by membership function. Subsequently, volatile components samples from 30 detected via...

The covert mortality nodavirus (CMNV), an emerging pathogen that infects several species severely in aquaculture including marine shrimps, freshwater prawns and crabs, has been detected both wild Artemia commercial cysts. Utilization of from unknown sources can impose considerable biosecurity risks hatchery operations, therefore effective hatching methods to eliminate the pathogenic potential CMNV-positive (CMNV+) cysts are urgently needed. In this work, we assessed efficacy three treatment...

In an established hepatocyte lipid deposition heat stress model, the expression levels of METTL3 and FTO were significantly upregulated (p < 0.05), indicating that play important roles in process hepatocytes. Transcriptome metabolome analyses showed had significant effects on linoleic acid, linolenic glycerophospholipid, arachidonic acid metabolic pathways After knockdown, m6A methylation level decreased, but difference was not > FABP4 Accα increased, HSP60, HSP70, HSP110 decreased...

N6-methyladenosine (m6A) modification of RNA is a critical post-transcriptional modification, that dynamically contributes to testicular development and spermatogenesis. Nevertheless, the investigation into role m6A in sheep remains insufficient. Herein, we conducted comprehensive analysis transcriptome landscape testes F1 hybrid Southdown × Hu across M0 (0 months old, newborn), M3 (3 sexually immature), M6 (6 mature), Y1 (1 years adult). By profiling signatures transcriptome, observed...

Prolonged natural selection and artificial breeding have contributed to increased uniformity within the Tibetan sheep population, resulting in a reduction genetic diversity establishment of selective signatures genome. This process has led loss heterozygosity specific genomic regions formation Runs Homozygosity (ROH). Current research on ROH predominantly focuses inbreeding signals selection; however, there is paucity investigation into load pressures associated with ROH, both these beyond....

The covert mortality nodavirus (CMNV), an emerging pathogen that infects several species severely in aquaculture, including marine shrimps, freshwater prawns, and crabs, has been detected both wild Artemia commercial cysts. Utilization of from unknown sources can impose considerable biosecurity risks hatchery operations; therefore, effective hatching methods to eliminate the pathogenic potential CMNV-positive (CMNV+) cysts are urgently needed. In this work, we assessed efficacy three...

With the intensive development of sheep industry and increasing global temperatures, heat stress in has become an increasingly severe important issue recent years. The level N6-methyladenosine (m6A) RNA methylation changes response to plays roles responses. However, role m6A remains unclear. To explore this issue, we measured protein (HSP) expression, liver function indexes, on RNA, m6A-related enzyme tissue damage that had been subjected stress. At transcriptome level, our results showed...

Inner Mongolia and Liaoning cashmere goats in China are well-known for their quality yield. Thus, they great models identifying genomic regions associated with traits. Herein, 53 goats, Huanghuai were genotyped, 53,347 single-nucleotide polymorphisms (SNPs) produced using the Illumina Caprine 50K SNP chip. Additionally, we identified some positively selected SNPs by analyzing Fst XP-EHH. The top 5% of had selection signatures. After gene annotation, 222 173 candidate genes respectively....

Tibetan goat is an ancient breed, which inhabits the adverse conditions of plateaus in China. To investigate role selection shaping its genomes, we genotyped goats (Nagqu Prefecture, above 4500 m) and three lowland populations (Xinjiang goats, Taihang Huanghuai goats). The result PCA, neighbor-joining (N-J) tree model-based clustering showed that genetic structure between has significant difference. As demonstrated by di statistic, found some genes were related to high-altitude adaptation...

Abstract Background Copy number variation (CNV) is an important source of genetic that has a significant influence on phenotypic diversity, economically traits and the evolution livestock species. In this study, genome-wide CNV distribution characteristics 32 fine-wool sheep from three breeds were analyzed using resequencing. Results A total 1,747,604 CNVs detected in 7228 regions (CNVR) obtained after merging overlapping CNVs; these accounted for 2.17% reference genome. The average length...

MicroRNA (miRNA) is a kind of noncoding RNA whose function involved in various biological processes neuronal maturation and adipocyte cells, such as differentiation, proliferation, development, apoptosis, metabolism. Herein, miRNA-Seq was used to identify miRNAs the tail fat tissue Hu sheep (short-fat-tailed) Tibetan (short-thin-tailed). In this study, 155 differentially expression (DE miRNAs) were identified, including 78 up-regulated 77 down-regulated. Among these DE miRNAs, 17 reported...

Crossbreeding improves and enhances meat quality is widely used in sheep production; however, the molecular mechanisms underlying of various crossbred remain unknown. In this study, male Southdown, Suffolk Hu were with female sheep, transcriptomes metabolomes longissimus dorsi muscle F1 generation sequenced to explore how different sire breeds affect quality. The results showed that 631 differentially expressed genes 119 significantly altered metabolites contributed development...

Wool fineness affects the quality of wool, and some studies have identified about forty candidate genes that affect sheep wool fineness, but these often reveal only a certain proportion variation in thickness. We further explore additional associated with wool. Whole-genome resequencing eight breeds was performed to selection signals including four coarse fine/semi-fine breeds. Multiple methods (Fst θπ Ratio XP-EHH) were applied for traits. In total, 269 319 annotated fine (F vs. C) group (C...

Wool color is controlled by a variety of genes. Although the gene regulation some wool colors has been studied in relative depth, there may still be unknown genetic variants and control genes for or different breeds that need to identified recognized whole genome resequencing. Therefore, we used resequencing data compare analyze sheep populations population differentiation index nucleotide diversity ratios (Fst θπ ratio) as well extended haplotype purity between (XP-EHH) reveal selection...

Summary To obtain more knowledge on the origin and genetic diversity of swamp buffalo ( Bubalus bubalis ) in China, complete mitochondrial D‐loop sequences 119 samples representing seven native types were compared. Two DNA (mtDNA) lineages (lineages A B) determined for Chinese buffalo. Examination patterns suggest that lineage has undergone a population expansion event. Divergence B was estimated at 18 000 years ago. Combined analyses mtDNA from Chinese, Indian, Brazilian/Italian Southeast...

With the intensified and large-scale development of sheep husbandry global warming, heat stress has become an increasingly important issue. However, little is known about molecular mechanisms related to responses stress. In this study, transcriptomic analysis liver tissues in presence absence was conducted, with goal identifying genes pathways regulation when under such After a comparison reference genome, 440,226,436 clean reads were obtained from eight libraries. A p-value ≤ 0.05 fold...

Hybridization of livestock can be used to improve varieties, and different hybrid combinations produce unique breeding effects. In this study, male Southdown Suffolk sheep were selected hybridize with female Hu explore the effects parentage on muscle growth development offspring. Using data-independent acquisition technology, we identified 119, 187, 26 differentially abundant proteins (DAPs) between × (HH) versus (NH), HH (SH), NH SH crosses. Two DAPs, MYOZ2 MYOM3, common three groups mainly...

The aim of this study was to examine the correlation between platelet-derived growth factor-D (PDGF-D) gene and sheep tail type character explore potential underlying mechanism. A total 533 were included in study. Polymorphic sites examined by Pool-seq, individual genotype identification analysis data conducted using matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF-MS) method. JASPART website used predict transcription factor binding promoter region...

Hu sheep and Tibetan in China are characterized by fat tails thin tails, respectively. Several transcriptomes have been conducted different breeds to identify the differentially expressed genes (DEGs) underlying this trait. However, these studies identified DEGs breeds.Hence, RNA sequencing was performed on sheep. We obtained a total of 45.57 43.82 million reads, Two libraries mapped reads from 36.93 38.55 after alignment reference sequences. 2108 were identified, including 1247...