Nanotechnology is a multidisciplinary field evolved within past few decades and playing substantial role in environment, industry, agriculture pharmacology. Integration of nanotechnology biotechnology has led to the foundation nanomedicine. It provided novel breakthroughs cure various ailments disorders, drug delivery systems, detection diagnostics different diseases. However, use microbially synthesized nanoparticles health medicine still limited. This article highlights green approach...

Present study was aimed at screening and characterizing thermostable amylase-producing bacteria from water sediment samples of unexplored hot spring Tatta Pani Kotli Azad Kashmir. Four thermophilic isolates were characterized on morphological, biochemical, physiological basis authenticated by molecular analysis. By 16S rDNA sequencing, identified as Anoxybacillus mongoliensis (MBT001), flavithermus (MBT002), Bacillus (MBT004). Among all strains, MBT003 showed maximum homology with both...

Phytoremediation assisted with plant growth promoting bacteria (PGPB) is a green technology to remediate metal contaminated soils. Plants usually produce secondary metabolites tolerate toxicity. Present study was designed explore the phytoremediation potential of Vigna radiata var. NM-II in presence resistant PGPB and comparison produced under heavy stresses (Pb, Ni, Cr). Three selected for present include Bacillus pumilus MB246, Serratia nematodiphila MB307 Delftia Lacustris MB322. Pot...

Abstract Acidithiobacillus ferrooxidans , a chemolithoautotrophic bacterium, is well known for its mineral oxidizing properties. The current study combines experimental and whole genome sequencing approaches to investigate an iron oxidizing, extreme acidophilic A . isolate (IO-2C) from acid seep area near Carlos, TX, USA. Strain IO-2C was capable of i.e. sulphate ammonium yielding shwertmannite jarosite minerals. Further, the bacterium’s sequenced, assembled annotated general features,...

Biosurfactants are a diverse group of amphiphilic compounds obtained from microbes. In the present study, genomic analysis biosurfactant-producing Bacillus subtilis MB415 and MB418 oil-contaminated soil was performed. Initially, strains were screened for biosurfactant production by hemolytic assay, emulsification index, oil displacement. Further FTIR extracted biosurfactants revealed presence lipopeptides. The sequenced genomes 4.2 Mbps with 43% GC content. Among more than 4,500...

The exponential increase in the prevalence of multidrug resistant bacteria has resulted limiting surgical treatment options globally, potentially causing biofilm-related complications, implant failure, and severe consequences. This study aims to isolate characterize from post-surgical orthopaedic infections screening for multiple antibiotic resistance. A cross-sectional was conducted, involving isolation forty-four dominant pathogenic bacterial isolates 16 infected samples across Islamabad...

The extensive and unchecked application of chlorpyrifos against crop insects has caused contamination various ecosystems, such as soil, sediments, water, posing harm to plants, animals, useful arthropods, humans. present study aimed at evaluating the ability proto-type constructed wetland biodegrade its major metabolites especially 2-hydroxy-3, 5, 6-trichloropyridine/ol (TCP) using chlorpyrifos-degrading indigenous bacterial strains, namely, Acinetobacter baumanni Bacillus cibi with Canna...

<title>Abstract</title> Microorganisms are found to exist everywhere including extreme habitats where other forms of life rarely thrive. In this study, culture-independent technique was used analyze the microbial diversity water samples from Tata Pani hot spring in district Kotli Azad Kashmir, Pakistan. Physiochemical analysis also conducted study community composition and environment. Metagenomic sequencing five different yielded 875,184 16S rRNA sequence reads. The original output files...

Abstract Organic biomass is rich in cellulose and it the most abundant substrate for biofuel production. To address challenges posed by cellulosic biomass, cellulolytic microorganisms help improve feasibility of production from biomass. Present research was aimed to assess cellulase activity thermophilic isolates discovered Tata Pani hot spring, Kotli AJ&amp;K. Four bacteria were screened thermostable on basis clear zone formation with 1 % CMC. Selected strains Anoxybacillus mongoliensis...

High concentrations of metals strongly influence the composition soil microflora. Tanneries being one major sources metal pollution in Pakistan pose serious threat to bacterial communities. Present study aimed investigate diversity from tannery effluents affected soil. Total Sixteen isolates were purified and characterized morphologically biochemically. All selected facultative anaerobes able grow wide pH ranges 5-11. Only TS3, TS4, TS8, TS9, TS13 resistant Erythromycin. On basis phenotypic...

Microorganisms thrive nearly everywhere including extreme environments where few other forms of life can exist. Geochemistry sites plays a major role in shaping these microbial communities and microbes thriving such harsh conditions are untapped sources novel biomolecules. To understand the structure composition communities, culture-independent bacterial diversity was characterised for two Pakistan, Khewra salt range Murtazaabad hot spring. Barcoded amplicon sequencing technique used to...

Hot water springs are unique areas populated by mesophiles, thermotolerant, and hyperthermophiles. They source of diversity thermophiles, mainly belonging to archaea bacteria domains. The thermophiles gives an outline the huge biological potential that can be exploited for industrial applications.To this end, study was aimed isolate characterize unexplored thermophilic microorganisms from hot spring in Tatapani, Tehsil &amp; District Kotli AJK, Pakistan. Around 10 bacterial isolates were...

Hot water springs are unique areas populated by mesophiles, thermotolerant and hyperthermophiles. They the source of diversity thermophiles, mainly belonging to archaea bacteria domains. The thermophiles gives an outline huge biological potential that can be exploited for industrial applications.To this end, study was aimed isolate characterise unexplored thermophilic microorganisms from hot spring in Tatapani, Tehsil &amp; District Kotli AJK, Pakistan. Around 10 bacterial isolates were...

Peanut, the only cash crop of rainfed areas Pakistan, is facing immense challenges due to global warming. Climatic factors particularly temperature fluctuations and rain pattern shift significantly impact production yield peanut unavailability resilient varieties exacerbate this impact. To deal with cropping change losses, climate vagaries, a study was conducted develop early maturing hybrids using line into tester mating design. The F1 from parental lines were produced in year 2018 Line ×...

Study was aimed at screening and characterizing thermostable amylase-producing bacteria from water sediment samples of unexplored hot spring.. Four thermophilic isolates were characterized using 16S rDNA sequencing identified as Anoxybacillus mongoliensis (MBT001), flavithermus (MBT002), Bacillus (MBT004) MBT003 gave resemblance to both . Amylase activity measured biosynthesis observed 7 pH, 70 °C, 1.25% substrate concentration, 300 µL inocula size after 48 hours incubation. Optimum amylase...