A5079964473- Genetic and Kidney Cyst Diseases
- Cellular transport and secretion
- Advanced Fluorescence Microscopy Techniques
- Biotin and Related Studies
- Genetic Syndromes and Imprinting
- Pancreatic function and diabetes
- Advanced Electron Microscopy Techniques and Applications
- Physiological and biochemical adaptations
- Microtubule and mitosis dynamics
- Micro and Nano Robotics
- Photoacoustic and Ultrasonic Imaging
- Photosynthetic Processes and Mechanisms
- Insect Pest Control Strategies
- Protist diversity and phylogeny
- Retinal Development and Disorders
- Ubiquitin and proteasome pathways
- Insect-Plant Interactions and Control
- Aquatic Ecosystems and Biodiversity
- Cellular Mechanics and Interactions
- Extracellular vesicles in disease
- Insect Resistance and Genetics
University of California, San Francisco
2020-2023
University of Zurich
2017-2018
Chemical defense is one of the most important traits, which endow insects ability to conquer a diverse set ecological environments. secretions are used for against anything from vertebrate or invertebrate predators prokaryotic eukaryotic parasites food competitors. Tenebrionid beetles especially prolific in this category, producing several varieties substituted benzoquinone compounds. In order get better understanding genetic and molecular basis defensive secretions, we performed RNA...
The G protein–coupled receptor melanocortin-4 (MC4R) and its associated protein melanocortin receptor–associated 2 (MRAP2) are essential for the regulation of food intake body weight in humans. MC4R localizes functions at neuronal primary cilium, a microtubule-based organelle that senses relays extracellular signals. Here, we demonstrate MRAP2 is critical weight-regulating function neurons ciliary localization MC4R. More generally, our study also reveals GPCR to cilia can require specific...
ABSTRACT Cilia sense and transduce sensory stimuli, homeostatic cues developmental signals by orchestrating signaling reactions. Extracellular vesicles (EVs) that bud from the ciliary membrane have well-studied roles in disposal of excess material, most dramatically exemplified shedding micrometer-sized blocks photoreceptors. Shedding EVs cilia also affords cells with a powerful means to shorten cilia. Finally, cilium-derived may enable cell–cell communication variety organisms, ranging...
Ciliopathies are human disorders caused by dysfunction of primary cilia, ubiquitous organelles involved in transduction environmental signals such as light sensation photoreceptors. Concentration signal detection proteins opsins the ciliary membrane is achieved RabGTPase-regulated polarized vesicle trafficking and a selective barrier at base, transition zone (TZ). Dysfunction TZ protein CC2D2A causes Joubert/Meckel syndromes humans loss localization animal models, including retinal The link...
Ciliopathies are human disorders caused by dysfunction of primary cilia, ubiquitous microtubule-based organelles involved in signal transduction. Cilia anchored inside the cell through basal bodies (BBs), modified centrioles also acting as microtubule-organization centers. Photoreceptors (PRs) sensory neurons, whose cilium forms a highly specialized compartment called outer segment (OS) responsible for sensing incoming light. Thus, ciliopathies often present with retinal degeneration....
We present a method to investigate the subcellular protein localization in larval zebrafish retina by combining super-resolution light microscopy and scanning electron microscopy. The sub-diffraction limit resolution capabilities of microscopes allow improving accuracy correlated data. Briefly, 110 nanometer thick cryo-sections are transferred silicon wafer and, after immunofluorescence staining, imaged Subsequently, sections preserved methylcellulose platinum shadowed prior imaging...
We present a method to investigate the subcellular protein localization in larval zebrafish retina by combining super-resolution light microscopy and scanning electron microscopy. The sub-diffraction limit resolution capabilities of microscopes allow improving accuracy correlated data. Briefly, 110 nanometer thick cryo-sections are transferred silicon wafer and, after immunofluorescence staining, imaged Subsequently, sections preserved methylcellulose platinum shadowed prior imaging...
ABSTRACT The G protein-coupled receptor MC4R (Melanocortin-4 Receptor) and its associated protein MRAP2 (Melanocortin Receptor-Associated Protein 2) are both essential for the regulation of food intake body weight in humans mice. localizes functions at neuronal primary cilium, a microtubule-based organelle that senses relays extracellular signals. Here, we demonstrate is critical ciliary localization weight-regulating function MC4R. Our data reveal GPCR to cilia can require specific...
Abstract Genetic studies in humans and mice have demonstrated that the Melanocortin 4 Receptor (MC4R) is essential for adequate regulation of food intake body weight. MC4R expressed a small population hypothalamic neurons very little known about its molecular cellular dynamics vivo. We recently localizes to functions at primary cilia select control energy homeostasis. The cilium solitary hair-like organelle serves as an antenna sensing extracellular environment. Defective lead series...
PCP signaling polarizes epithelial cells within the plane of an epithelium. Core components adopt asymmetric subcellular localizations to both polarize and coordinate polarity between cells. Achieving asymmetry requires additional effectors, including some mediating post-translational modifications core components. Identification such proteins is challenging due pleiotropy. We used mass spectrometry-based proximity labeling proteomics identify regulators in Drosophila wing. identified...