A5039029363- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Ion Channels and Receptors
- Lipid Membrane Structure and Behavior
- Neurobiology and Insect Physiology Research
- Electrochemical Analysis and Applications
- Nicotinic Acetylcholine Receptors Study
- Metal complexes synthesis and properties
- Photoreceptor and optogenetics research
- Magnetism in coordination complexes
- Iron oxide chemistry and applications
- Plant Stress Responses and Tolerance
- stochastic dynamics and bifurcation
- Ion Transport and Channel Regulation
- X-ray Diffraction in Crystallography
- Mass Spectrometry Techniques and Applications
- Minerals Flotation and Separation Techniques
- Magnetic Properties and Synthesis of Ferrites
- Biochemical Analysis and Sensing Techniques
- Crystal structures of chemical compounds
- Crystallization and Solubility Studies
- Spectroscopy and Quantum Chemical Studies
- Connexins and lens biology
University of Valparaíso
2016-2025
Valparaiso University
2010-2025
Washington University in St. Louis
2022
University of Vermont
2022
University of Chile
2000-2016
Fraunhofer Chile Research Foundation
2016
Universidad Andrés Bello
2016
Centro Científico Tecnológico de Valparaíso
2014-2015
University of Bari Aldo Moro
2013
Centro de Estudios Científicos
1999-2008
Maxi-K channels consist of a pore-forming alpha subunit and regulatory beta subunit, which confers the channel with higher Ca(2+) sensitivity. Estradiol bound to activated (hSlo) only when both subunits were present. This activation was independent generation intracellular signals could be triggered by estradiol conjugated membrane-impenetrable carrier protein. study documents direct interaction hormone voltage-gated provides molecular mechanism for modulation vascular smooth muscle estrogens.
The gating kinetics of a Ca2+-activated K+ channel from adult rat muscle plasma membrane are studied in artificial planar bilayers. Analysis single-channel fluctuations distinguishes two Ca2+- and voltage-dependent processes: (a) short-lived closure (less than 1 ms) events appearing bursting pattern; (b) opening closing ranging one to several hundred milliseconds duration. latter process is independently the first denoted as primary mode. At constant voltage, mean open time mode linear...
The cold and menthol receptor, TRPM8, also designated CMR1, is a member of the transient receptor potential (TRP) family excitatory ion channels. TRPM8 channel activated by temperatures, voltage, menthol. In this study, we characterize cold- voltage-induced activation in an attempt to identify temperature- voltage-dependent components involved activation. Under equilibrium conditions, decreasing temperature has two effects. ( i ) It shifts normalized conductance vs. voltage curves toward...
Addition of membrane vesicles prepared from transverse tubule (T-tubule) membranes rabbit skeletal muscle to the aqueous phase a planar lipid bilayer induces stepwise increase in conductance. This conductance is both voltage and Ca2+ dependent. At 1 mM Ca2+, steady-state maximal at voltages higher than +20 mV decreases for more negative voltages. (Voltages refer side which are added, cis) Decreasing concentration reversibly shifts conductance-voltage curve toward right along axis....
Temperature transduction in mammals is possible because of the presence a set temperature-dependent transient receptor potential (TRP) channels dorsal root ganglia neurons and skin cells. Six thermo-TRP channels, all characterized by their unusually high temperature sensitivity ( Q 10 > 10), have been cloned: TRPV1–4 are heat activated, whereas TRPM8 TRPA1 activated cold. Because lack structural information, molecular basis for regulation remains unknown. In this study, we assessed role...
The interaction of Ca2+ and Ba2+ with a Ca2+-activated K+ channel from rabbit skeletal muscle membranes is studied in planar lipid bilayers. At [Ca2+] greater than or equal to 100 microM the cis side (the which vesicles are added) at positive voltages, kinetics consisted bursts activity interrupted by long periods quiescence. We found that reciprocal mean burst time increases linearly [Ca2+], whereas for quiescent (closed) independent [Ca2+]. number reduced increasing [K+]. Micromolar...
Phosphatidylinositol 4,5-bisphosphate (PIP2) plays a central role in the activation of several transient receptor potential (TRP) channels. The PIP2 on temperature gating thermoTRP channels has not been explored detail, and process is largely unexplained. In this work, we have exchanged different segments C-terminal region between cold-sensitive (TRPM8) heat-sensitive (TRPV1) channels, trying to understand segment activation. A chimera which proximal part TRPV1 replaces an equivalent section...
Prolonged depolarization induces a slow inactivation process in some K+ channels. We have studied ionic and gating currents during long depolarizations the mutant Shaker H4-Δ(6–46) channel nonconducting (Shaker H4-Δ(6–46)-W434F). These channels lack amino terminus that confers fast (N-type) (Hoshi, T., W.N. Zagotta, R.W. Aldrich. 1991. Neuron. 7:547–556). Channels were expressed oocytes measured with cut-open-oocyte patch-clamp techniques. In both clones, curves describing voltage dependence...
Hypertension is the most prevalent risk factor for cardiovascular diseases, present in almost 30% of adults. A key element control vascular tone large-conductance, Ca(2+)-dependent K(+) (BK) channel. The BK channel smooth muscle formed by an ion-conducting alpha subunit and a regulatory beta(1) subunit, which couples local increases intracellular Ca(2+) to augmented activity relaxation. Our large population-based genetic epidemiological study has identified new single-nucleotide substitution...
Denervation of skeletal muscles induces atrophy, preceded by changes in sarcolemma permeability causes not yet completely understood. Here, we show that denervation-induced Evans blue dye uptake vivo fast, but slow, myofibers was acutely inhibited connexin (Cx) hemichannel/pannexin1 (Panx1) channel and purinergic ionotropic P2X7 receptor (P2X7R) blockers. Denervated showed up-regulation Panx1 de novo expression Cx39, Cx43, Cx45 hemichannels as well P2X7Rs transient potential subfamily V,...