A5080343219- Muscle Physiology and Disorders
- Spinal Cord Injury Research
- Peptidase Inhibition and Analysis
- Muscle activation and electromyography studies
- Adipose Tissue and Metabolism
- Ubiquitin and proteasome pathways
- Exercise and Physiological Responses
- Cerebral Palsy and Movement Disorders
- Genetic Neurodegenerative Diseases
- Bone Metabolism and Diseases
- Neurogenetic and Muscular Disorders Research
- Protease and Inhibitor Mechanisms
- MicroRNA in disease regulation
- Hormonal and reproductive studies
- Cardiomyopathy and Myosin Studies
- Signaling Pathways in Disease
- Mitochondrial Function and Pathology
- Stroke Rehabilitation and Recovery
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- RNA Research and Splicing
- Neuropeptides and Animal Physiology
- Circular RNAs in diseases
- Kruppel-like factors research
- Endoplasmic Reticulum Stress and Disease
- Neuroscience of respiration and sleep
James J. Peters VA Medical Center
2015-2024
Icahn School of Medicine at Mount Sinai
2015-2024
Pain and Rehabilitation Medicine
2023
Bronx Veterans Medical Research Foundation
2020-2021
Birmingham VA Medical Center
2021
The Bronx Defenders
2009-2021
Rehabilitation Research and Development Service
2004-2020
Center for Excellence in Education
2016
United States Department of Veterans Affairs
1989-2011
Philanthropy New York
2000
<h3>Objectives</h3> To explore mechanisms through which altered peroxisome proliferator–activated receptor γ coactivator 1α (PGC-1α) expression may influence Alzheimer disease (AD) amyloid neuropathology and to test the hypothesis that promotion of PGC-1α in neurons might be developed as a novel therapeutic strategy AD. <h3>Design</h3> Case-control. <h3>Patients</h3> Human postmortem brain (hippocampal formation) samples from AD cases age-matched non-AD cases. <h3>Results</h3> Using...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEvidence for the presence of five distinct proteolytic components in pituitary multicatalytic proteinase complex. Properties two cleaving bonds on carboxyl side branched chain and small neutral amino acidsMarian Orlowski, Christopher Cardozo, Charlene MichaudCite this: Biochemistry 1993, 32, 6, 1563–1572Publication Date (Print):February 1, 1993Publication History Published online1 May 2002Published inissue 1 February...
Significance The apolipoprotein E4 (ApoE4) genotype is the strongest genetic risk factor for developing Alzheimer’s disease (AD). However, mechanisms that underlie this link between ApoE4 and AD are not well understood. Our data in paper first mechanistic studies to our knowledge genotype-specific changes brain phospholipid homeostasis ApoE4-increased susceptibility develop AD. indicate previously unidentified therapeutic options treatment of AD, targeting ApoE4’s pathogenic nature.
Denervation of skeletal muscles induces atrophy, preceded by changes in sarcolemma permeability causes not yet completely understood. Here, we show that denervation-induced Evans blue dye uptake vivo fast, but slow, myofibers was acutely inhibited connexin (Cx) hemichannel/pannexin1 (Panx1) channel and purinergic ionotropic P2X7 receptor (P2X7R) blockers. Denervated showed up-regulation Panx1 de novo expression Cx39, Cx43, Cx45 hemichannels as well P2X7Rs transient potential subfamily V,...
Abstract Denervation of skeletal muscles induces severe muscle atrophy, which is preceded by cellular alterations such as increased plasma membrane permeability, reduced resting potential and accelerated protein catabolism. The factors that induce these changes remain unknown. Conversely, functional recovery following denervation depends on successful reinnervation. Here, we show activation nicotinic acetylcholine receptors (nAChRs) quantal release (ACh) from motoneurons sufficient to...
Amino acid sequencing of subunits the multicatalytic proteinase complex (MPC) isolated from bovine spleen showed an almost complete replacement X, Y, and Z subunits, constitutively expressed in most tissues, by interferon-γ-inducible LMP7, LMP2, MECL1 subunits. A comparison with pituitary MPC found a decreased chymotrypsin-like activity, depressed peptidylglutamyl-peptide hydrolyzing highly active component properties similar to, but not identical with, that branched chain amino preferring...
Testosterone stimulates the expression of IGF-I in cells and tissues that include prostate, muscle satellite cells, uterus. Here, molecular mechanisms this effect testosterone were explored. increased mRNA levels HepG2 LNCaP stimulated activity reporter genes controlled by 1.6 kb upstream promoter human gene. An androgen-responsive region was located between -1320 -1420 bases first codon identified truncation studies. The found to contain two sequences resembling known androgen receptor...
Exosomes are vesicles released by many eukaryotic cells; their cargo includes proteins, mRNA and microRNA (miR) that can be transferred to recipient cells regulate cellular processes in an autocrine or paracrine manner. While of the myoblast lineage secrete exosomes, it is not known whether skeletal muscle fibers (myofibers) release exosomes. In this study, we found cultured myofibers nanovesicles have bilamellar membranes average size 60-130 nm, contain typical exosomal proteins miRNAs...
Evidence indicates that a component of the multicatalytic proteinase complex (MPC) preferentially cleaves bonds after branched chain amino acids (BrAAP) is major factor responsible for protein-degrading activity MPC. We report here synthesis substrate-related peptidyl aldehydes inhibit this toward both synthetic peptide substrates and proteins. The most potent inhibitors, Cbz-Gly-Pro-Phe-leucinal (Cbz-GPFL-CHO) inhibits competitively with Ki 1.5 microM. also small neutral acid preferring...
Glucocorticoids are a well-recognized and common cause of muscle atrophy that can be prevented by testosterone. However, the molecular mechanisms underlying such protection have not been described. Thus, global effects testosterone on dexamethasone-induced changes in gene expression were evaluated rat gastrocnemius using DNA microarrays. Gene was analyzed after 7-d administration dexamethasone, dexamethasone plus testosterone, or vehicle. Dexamethasone changed 876 probe sets at least 2-fold....
Mechanisms by which muscle regulates bone are poorly understood.Electrically stimulated contraction reversed elevations in resorption and increased Wnt signaling bone-derived cells after spinal cord transection.Muscle reduced of unloaded independently the CNS, through mechanical effects and, potentially, nonmechanical signals (e.g. myokines).The study provides new insights regarding muscle-bone interactions. Muscle work as a functional unit. Cellular molecular mechanisms underlying activity...
The glucose transporter 4 (Glut4) mediates insulin-dependent uptake. Glut4 expression levels are correlated with whole-body homeostasis. Insulin signaling is known to recruit the cell surface. Expression of subject tissue-specific hormonal and metabolic regulation. molecular mechanisms regulating skeletal muscle remain be elucidated. Myostatin (Mstn) reported involved in regulation energy metabolism. While elevated Mstn associated obesity type-2 diabetes both human mouse models, null mice...
The purpose of the work is to investigate effects low-dose testosterone replacement therapy (TRT) and evoked resistance training (RT) on body composition metabolic variables after spinal cord injury (SCI). Twenty-two individuals with chronic motor complete SCI (ages 18-50 years) were randomly assigned either TRT+RT (n = 11) or TRT for 16 weeks following a 4 -week delayed entry period. men underwent twice weekly progressive RT using electrical stimulation ankle weights. was administered via...
Hsp90 functions in association with several cochaperones for folding of protein kinases and transcription factors, although the relative contribution each to overall reaction is unknown. We assayed role nine different activation Ste11, a Saccharomyces cerevisiae mitogen-activated kinase kinase. Studies on signaling via this pathway was measured by alpha-factor-stimulated induction FIG1 or lacZ, repression HHF1. Several cochaperone mutants tested had reduced HHF1 repression, differing...