A5091585522- Pancreatic and Hepatic Oncology Research
- Cardiac Imaging and Diagnostics
- Receptor Mechanisms and Signaling
- Coronary Interventions and Diagnostics
- Lung Cancer Research Studies
- Anesthesia and Sedative Agents
- Cancer Genomics and Diagnostics
- Neuroendocrine Tumor Research Advances
- Cardiac, Anesthesia and Surgical Outcomes
- Neuroscience and Neuropharmacology Research
- Acute Kidney Injury Research
- Lung Cancer Treatments and Mutations
- Anesthesia and Pain Management
- Acute Myocardial Infarction Research
- Advanced X-ray and CT Imaging
- Anesthesia and Neurotoxicity Research
- Nanoplatforms for cancer theranostics
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Intensive Care Unit Cognitive Disorders
- Chronic Kidney Disease and Diabetes
- Peripheral Artery Disease Management
- Pain Mechanisms and Treatments
- Cardiac Arrest and Resuscitation
- Epigenetics and DNA Methylation
- Nitric Oxide and Endothelin Effects
University of Ottawa
2018-2025
Ottawa Hospital
2024-2025
University of Toronto
2024
Princess Margaret Cancer Centre
2024
Centre Hospitalier de l’Université de Montréal
2024
Ottawa Hospital Research Institute
2024
HealthPartners
2024
University Health Network
2024
London School of Hygiene & Tropical Medicine
2024
Novel (United States)
2020-2022
The delta(B) and delta(C) splice variants of Ca(2+)/calmodulin-dependent protein kinase II (CaMKII), which differ by the presence a nuclear localization sequence, are both expressed in cardiomyocytes. We used transgenic (TG) mice CaMKII expression cardiomyocytes to test hypothesis that CaMKIIdelta(C) isoform regulates cytosolic Ca(2+) handling isoform, localizes nucleus, gene transcription. Phosphorylation sites on ryanodine receptor (RyR) phospholamban (PLB) were increased TG. This was...
Women are at a higher risk for bleeding/vascular complications (VC) related to cardiovascular procedures. Although the overall incidence of percutaneous coronary intervention (PCI)-related bleeding/VC has declined, impact this decline, specifically in women, is unknown.We studied 13 653 female and 32 334 male consecutive cases, from 2002 2007, Northern New England PCI Registry. We sought (1) compare absolute rates women men over time, (2) define predictors undergoing PCI, (3) trend gender...
BACKGROUNDA patient-derived organoid (PDO) platform may serve as a promising tool for translational cancer research. In this study, we evaluated PDO's ability to predict clinical response gastrointestinal (GI) cancers.METHODSWe generated PDOs from primary and metastatic lesions of patients with GI cancers, including pancreatic ductal adenocarcinoma, colorectal cholangiocarcinoma. We compared PDO the observed donor same treatments.RESULTSWe report an approximately 80% concordance rate between...
In this work we have used fluorescence spectroscopic approaches to examine the binding of ByT subunit complex transducin photoreceptor, rhodopsin.To do this, covalently labeled flyT with environmentally sensitive fluorescent cysteine reagent 2-(4'-maleimidylani1ino)naphthalene-6-sulfonic acid (MIANS).By using MIANS moiety as a reporter group, were able monitor directly MIANS-ByT light-activated rhodopsin, which was reconstituted into phosphatidylcholine vesicles, through an enhancement...
We have made use of the enhancement intrinsic fluorescence alpha subunit transducin (alpha T), which accompanies guanine nucleotide exchange, to follow reconstituted interactions between pure rhodopsin and in phospholipid vesicles. When T.GDP complex is added lipid vesicles containing beta gamma T complex, a light- guanosine 5'-O-(3-thiotriphosphate) (GTP S)-dependent emission observed. GTP substituted for S, similar occurs; however, this transient precedes decay complete 2-5 min. The fact...
The ideal procedural sedation drug for emergency department (ED) use would be easily titrated, rapid in onset, brief duration, and provide analgesia without respiratory or hemodynamic compromise. Although many agents have been tried, no single fits this profile. authors evaluated the comparative effectiveness safety of propofol versus propofol/ketamine combination using bispectral index monitoring measuring depth sedation. A prospective, randomized case series patients undergoing fracture...
Contrast-induced acute kidney injury (CI-AKI) is associated with increased morbidity and mortality after percutaneous coronary interventions a patient safety objective of the National Quality Forum. However, no formal quality improvement program to prevent CI-AKI has been conducted. Therefore, we sought determine whether 6-year regional multicenter intervention could reduce interventions.We conducted prospective study (serum creatinine increase ≥0.3 mg/dL within 48 hours or ≥50% during...
Adenosquamous cancer of the pancreas (ASCP) is a subtype pancreatic that has worse prognosis and greater metastatic potential than more common ductal adenocarcinoma (PDAC) subtype. To distinguish genomic landscape ASCP identify actionable targets for this lethal cancer, we applied DNA content flow cytometry to series 15 tumor samples including five patient-derived xenografts (PDX). We interrogated purified sorted fractions from these with whole-genome copy-number variant (CNV), whole-exome...
Pancreatic neuroendocrine tumors (pNETs) are rare but increasingly prevalent malignancies with varied prognoses and a diverse range of treatment options, including surgery, somatostatin analogues (SSAs), chemotherapy, targeted therapy, peptide receptor radionuclide therapy (PRRT). This retrospective cohort study analyzed patterns among 189 pNET patients treated between January 2010 June 2021 at two Canadian cancer centres: the Verspeeten Family Cancer Centre (VFCC), which offers PRRT, Ottawa...
BRAF V600E mutations are identified in 10 % of metastatic colorectal cancer (mCRC) cases. In this project, we evaluated the clinicopathologic features and natural history patients with mutant (BRAF-mt) mCRC prior to era targeted therapy. This is a retrospective project evaluating diagnosed an mutation between January 1, 2015 December 31, 2021 seen at Ottawa Hospital Cancer Centre approval cetuximab encorafenib Canada. Demographic, clinical, characteristics were collected from medical...
In order to investigate the possible roles of intracellular domains rhodopsin in functional coupling photoreceptor transducin, different peptides that correspond parts known sequence were synthesized. Since we have found tht binding alpha subunit transducin (alpha T) increases susceptibility T phosphorylation by protein kinase C-beta 1, used this reaction as an initial screen for mimic actions rhodopsin. The results indicated a peptide from C-terminal tail (amino acids 325-338;...
In these studies we have investigated the role of beta gamma T subunit complex in promoting rhodopsin-stimulated guanine nucleotide exchange reaction (i.e. activation event) alpha subunit. The results demonstrate that although increases association with lipid vesicles lack photoreceptor, is not necessary for binding to containing rhodopsin, provided sufficient amounts rhodopsin are present. rhodopsin-promoted GDP/guanosine 5'-O-(3-thiotriphosphate) (GTP S) reaction, within rhodopsin-alpha...
Thyrotropin-releasing hormone (TRH) stimulates prolactin production by GH4C1 rat pituitary tumor cells, which possess high-affinity membrane receptors for the peptide. TRH caused up to a 50% increase in activity of low-Km GTPase membranes from cells. The stimulatory effect was maximal at GTP concentrations 1 microM or lower. an between 0.2 and 20 pmol hydrolyzed per mg protein min, depending on concentration, while binding 0.3 pmol/mg protein. did not stimulate GH12C1, GH-Y two lines lacking...
This study evaluates the variation in practice patterns associated with contrast-induced acute kidney injury (CI-AKI) and identifies clinical practices that have been a reduction CI-AKI. Background CI-AKI is recognised as complication of invasive cardiovascular procedures events, prolonged hospitalisation, end-stage renal disease, all-cause mortality. Reducing risk patient safety objective set by National Quality Forum.This prospectively collected quantitative qualitative data from 10...
Abstract In the early years of diagnostic cardiac catheterization, strict sterile precautions were required for cutdown procedures. Thirteen ago, when original guidelines written, brachial arteriotomy was still frequently utilized, femoral closure devices uncommon, “implantables,” such as intracoronary stents and PFO/ASD devices, in their infancy, percutaneous valve replacement not a consideration. 2005, catheterization laboratory is complex interventional suite with access routine device...
The functional interactions of the retinal G protein, transducin, with cyclic GMP phosphodiesterase (PDE) have been examined using different purified subunit components transducin and native trypsin-treated forms effector enzyme. limited trypsin treatment PDE removes low molecular weight γ (Mr approximately 14,000) enzyme, yielding a catalytic moiety comprised two larger subunits (α, Mr ≈ 85,000–90,000; β, 85,000–90,000), which is insensitive to addition either pure αT·GTP S species or β γT...
The COURAGE (Clinical Outcomes Utilizing Revascularization and Aggressive Drug Evaluation) trial was designed to compare optimal medical therapy alone versus percutaneous coronary intervention (PCI) for treatment of patients with stable artery disease showed equal efficacy or without PCI. impact results from the on clinical practice is unknown.We analyzed 26 388 consecutive Northern New England Cardiovascular Disease PCI Registry who underwent between January 2006 June 2009. We identified a...