Natural animal populations are rarely screened for ploidy‐level variation at a scale that allows detection of potentially important aberrations common ploidy patterns. This type screening can be especially the many mixed sexual/asexual systems in which sexuals presumed to dioecious diploids and asexuals assumed triploid all‐female. For example, elevation level above triploidy source genetic raises possibility gene flow among levels asexual lineages. We used cytometry mtDNA sequencing...

The APC tumor suppressor regulates diverse stem cell processes including gene regulation through Wnt-β-catenin signaling and chromosome stability microtubule interactions, but how the disparate functions of are controlled is not well understood. Acting as part a pathway that controls asymmetric division, Caenorhabditis elegans APC, APR-1, promotes nuclear export β-catenin WRM-1 by asymmetrically stabilizing microtubules. Wnt function also depends on second β-catenin, SYS-1, which binds to C....

Failures of neural tube closure are common and serious birth defects, yet we have a poor understanding the interaction genetics cell biology during closure. Additionally, mutations that cause defects (NTDs) tend to affect anterior or posterior regions but rarely both, indicating regional specificity NTD genetics. To better understand behaviors closure, analyzed dynamic localization actin N-cadherin via high-resolution tissue-level time-lapse microscopy Xenopus investigate regionality gene...

ABSTRACT The Wnt/β-catenin signaling pathway is utilized across metazoans. However, the mechanism of signal transduction, especially dissociation β-catenin destruction complex by Dishevelled proteins, remains controversial. Here, we describe function paralogs DSH-2 and MIG-5 in asymmetry (WβA) Caenorhabditis elegans, where WβA drives asymmetric cell divisions throughout development. We find that redundantly regulate fate hypodermal seam cells. Similarly, both are required for positive...

Abstract Asymmetric cell division is critical during development, as it influences processes such fate specification and migration. We have characterized FRK-1, a homolog of the mammalian Fer nonreceptor tyrosine kinase, found to be required for differentiation maintenance epithelial types, including stem cell-like seam cells hypodermis. A genomic knockout frk-1, allele ok760, results in severely uncoordinated larvae that arrest at L1 stage an excess number lateral hypodermal appear lost...

Apical constriction results in apical surface reduction epithelial cells and is a widely used mechanism for morphogenesis. Both medioapical junctional actomyosin remodeling are involved constriction, but the deployment of medial versus their genetic regulation vertebrate embryonic development have not been fully described. In this study, we investigate dynamics by RhoGEF protein Plekhg5 Xenopus bottle cells. Using live imaging quantitative image analysis, show that assume different shapes,...

Abstract Apical constriction results in apical surface reduction epithelial cells and is a widely used mechanism for morphogenesis during embryo development. Both medioapical junctional actomyosin remodeling are involved constriction, but the deployment of medial versus specific developmental processes has not been fully described. Additionally, genetic regulation dynamics poorly understood vertebrate systems. In this study, we investigate their by RhoGEF protein Plekhg5 Xenopus bottle...

Abstract Failures of neural tube closure are common and serious birth defects, yet we have a poor understanding the interaction genetics cell biology during closure. Additionally, mutations that cause defects (NTDs) tend to affect anterior or posterior regions but rarely both, indicating regional specificity NTD genetics. To better understand behaviors closure, analyzed dynamic localization actin N-cadherin via high-resolution tissue-level time-lapse microscopy Xenopus investigate...

Abstract During neural tube closure, ectoderm cells constrict their apical surfaces to bend and fold the tissue into a that will become central nervous system. These are physically interconnected via N-cadherin, mutation of critical genes within relatively small numbers can result in closure defects due non-cell autonomous cell behavior defects. Despite this finding, we have poor understanding how neuroepithelial interact during constriction. In our previous paper, introduced an imaging...