A5020589585- Genetics, Aging, and Longevity in Model Organisms
- Wnt/β-catenin signaling in development and cancer
- Reproductive Biology and Fertility
- Developmental Biology and Gene Regulation
- Marine animal studies overview
- Congenital heart defects research
- Cancer-related gene regulation
- Microtubule and mitosis dynamics
- Hearing, Cochlea, Tinnitus, Genetics
- RNA Research and Splicing
- Circadian rhythm and melatonin
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- RNA regulation and disease
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Water Systems and Optimization
- Pharmaceutical industry and healthcare
- Global Public Health Policies and Epidemiology
- Ocular Infections and Treatments
- Global Maternal and Child Health
- 14-3-3 protein interactions
- Fungal and yeast genetics research
- Maternal and Neonatal Healthcare
- Signaling Pathways in Disease
University of Iowa
2014-2025
University of Tampa
2014
University of Wisconsin–Madison
2006-2009
Howard Hughes Medical Institute
2009
Texas A&M University
2001-2006
Philips (Finland)
2003
Northwestern University
1999
University of Illinois Chicago
1989
beta-Catenins are conserved regulators of metazoan development that function with TCF DNA-binding proteins to activate transcription. In Caenorhabditis elegans, SYS-1/beta-catenin and POP-1/TCF regulate several asymmetric divisions, including the somatic gonadal precursor cell (SGP). distal but not proximal SGP daughter, transcriptionally ceh-22 specify fate. Here, we investigate distribution its regulation. Using a rescuing transgene, VNS::SYS-1, which fuses VENUS fluorescent protein SYS-1,...
Asymmetric cell division (ACD) allows daughter cells of a polarized mother to acquire different developmental fates. In C. elegans, the Wnt/β-catenin Asymmetry (WβA) pathway regulates many embryonic and larval ACDs; here, Wnt gradient induces an asymmetric distribution signaling components within dividing cell. One terminal nuclear effector WβA is transcriptional activator SYS-1/β-catenin. SYS-1 sequentially negatively regulated during ACD; first by centrosomal regulation subsequent...
Induction of the otic placode, which gives rise to all tissues comprising inner ear, is a fundamental aspect vertebrate development. A number studies indicate that fibroblast growth factor (Fgf), especially Fgf3, necessary and sufficient for induction. However, an alternative model proposes Fgf must cooperate with Wnt8 induce differentiation. Using genetic approach in zebrafish, we tested roles Fgf8 Wnt8. We demonstrate localized misexpression either Fgf3 or ectopic placodes vesicles, even...
The valentino (val) mutation in zebrafish perturbs hindbrain patterning and, as a secondary consequence, also alters development of the inner ear. We have examined relationship between these defects and expression fgf3 fgf8 hindbrain. otic vesicle val/val mutants is smaller than normal, yet produces nearly twice normal number hair cells, some cells are produced ectopically anterior posterior maculae. Anterior markerspax5 nkx5.1 expressed expanded domains that include entire epithelium...
The APC tumor suppressor regulates diverse stem cell processes including gene regulation through Wnt-β-catenin signaling and chromosome stability microtubule interactions, but how the disparate functions of are controlled is not well understood. Acting as part a pathway that controls asymmetric division, Caenorhabditis elegans APC, APR-1, promotes nuclear export β-catenin WRM-1 by asymmetrically stabilizing microtubules. Wnt function also depends on second β-catenin, SYS-1, which binds to C....
Reveals that research using a new learning organisation benchmarking implementation model has found Australian organisations are increasingly committed to adopting, and implementing, principles. Discusses the debate over individual organisational learning. Outlines ten‐principle describes methodology used establish its validity. Shows how can determine extent of shortfall between importance accorded principles their implementation. It ability identify sectoral trends reveal weaknesses....
ABSTRACT The Wnt/β-catenin signaling pathway is utilized across metazoans. However, the mechanism of signal transduction, especially dissociation β-catenin destruction complex by Dishevelled proteins, remains controversial. Here, we describe function paralogs DSH-2 and MIG-5 in asymmetry (WβA) Caenorhabditis elegans, where WβA drives asymmetric cell divisions throughout development. We find that redundantly regulate fate hypodermal seam cells. Similarly, both are required for positive...
Abstract Asymmetric cell division is critical during development, as it influences processes such fate specification and migration. We have characterized FRK-1, a homolog of the mammalian Fer nonreceptor tyrosine kinase, found to be required for differentiation maintenance epithelial types, including stem cell-like seam cells hypodermis. A genomic knockout frk-1, allele ok760, results in severely uncoordinated larvae that arrest at L1 stage an excess number lateral hypodermal appear lost...
The Wnt/β-catenin pathway is a key cell signaling module utilized in fate specification. Recent studies suggest an interplay between components and centrosome function. This study explores the net effects of loss on Wnt posttranslational β-catenin regulation.
Abstract Asymmetric cell division (ACD) is a fundamental mechanism of fate specification and adult tissue homeostasis. In C. elegans , the Wnt/β-catenin asymmetry (WβA) pathway regulates ACDs throughout embryonic larval development. Under control Wnt ligand-induced polarity, transcription factor POP-1/TCF functions with coactivator SYS-1/β-catenin to activate gene expression in signaled or, absence coactivator, repress target genes unsignaled daughter cell. To date, investigation Groucho...