A5022448090- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Ion Channels and Receptors
- Alzheimer's disease research and treatments
- Mitochondrial Function and Pathology
- Photoreceptor and optogenetics research
- Cholinesterase and Neurodegenerative Diseases
- Neurogenesis and neuroplasticity mechanisms
- Trace Elements in Health
- Adipose Tissue and Metabolism
- Pain Mechanisms and Treatments
- Lipid Membrane Structure and Behavior
- Neurobiology and Insect Physiology Research
- Biochemical effects in animals
Perm State University
2017-2024
Weizmann Institute of Science
2021-2024
Laboratory of Molecular Genetics
2017
N. I. Lobachevsky State University of Nizhny Novgorod
2017
University of Bristol
2017
Dendritic spines are critical components of neuronal synapses as they receive and transform synaptic inputs into a succession calcium-regulated biochemical events. The spine apparatus (SA), an extension smooth endoplasmic reticulum, regulates slow fast calcium dynamics in spines. Calcium release events deplete SA ion reservoir rapidly, yet the next cycle signaling requires its replenishment. How achieve this replenishment without triggering remains unclear. Using computational modeling, STED...
There are growing indications for the involvement of calcium stores in plastic properties neurons and particularly dendritic spines central neurons. The store-operated entry (SOCE) channels assumed to be activated by sensor stromal interaction molecule (STIM)which leads activation its associated Orai channel. two STIM species, differential role SOCE is not entirely clear. In present study, we were able distinguish between transfected STIM1, which more mobile primarily young neurons, STIM2...
Presenilin 1 (PS1) is a transmembrane proteolytic subunit of γ-secretase that cleaves amyloid precursor proteins. Mutations in PS1 (mPS1) are associated with early-onset familial Alzheimer’s disease (AD). The link between mutated PS1, mitochondrial calcium regulation, and AD has been studied extensively different test systems. Despite the wide-ranging role mPS1 AD, there paucity information on neuronal cell death, hallmark AD. In present study, we employed selective uncoupler carbonyl...
While neuronal mitochondria have been studied extensively in their role health and disease, the rules that govern calcium regulation remain somewhat vague. In present study using cultured rat hippocampal neurons transfected with mtRCaMP mitochondrial sensor, we investigated effects of cytosolic surges on dynamics ([Ca2+]m). Cytosolic ([Ca2+]c) was measured high affinity sensor Fluo-2. We recorded two types events: local global ones. Local events were limited to a small, 2-5 µm section...
A major route for influx of calcium ions into neurons uses the STIM-Orai1 voltage-independent channel. Once cytosolic ([Ca2+]i) elevates, it activates mitochondrial and endoplasmic stores, to affect downstream molecular pathways. In present study we employed a novel drug, Carbonyl Cyanide Chlorophenylhydrazone (CCCP), uncoupler, explore role mitochondria in cultured neuronal morphology. CCCP caused sustained elevation [Ca2+]i and, quite surprisingly, massive increase density dendritic...
A major route for the influx of calcium ions into neurons uses STIM-Orai1 voltage-independent channel. Once cytosolic ([Ca2+]i) elevates, it activates mitochondrial and endoplasmic stores to affect downstream molecular pathways. In present study, we employed a novel drug, carbonyl cyanide chlorophenylhydrazone (CCCP), uncoupler, explore role mitochondria in cultured neuronal morphology. CCCP caused sustained elevation [Ca2+]i and, quite surprisingly, massive increase density dendritic...
Glutamatergic signaling is one of the main types excitatory synaptic transmission in brain.It plays a key role normal brain function and cognitive performance.Glutamatergic failure associated with disorders; therefore this system considered an essential target therapeutic interventions.Glutamatergic mediated by set ionotropic metabotropic glutamate receptors including kainate receptors.These (both metabotropic) are involved process modulating excitation/inhibition balance.The modulatory...
Abstract Synaptopodin (SP) is localized within the spine apparatus, an enigmatic structure located in neck of spines central excitatory neurons. It serves as a link between head, where synapse located, and endoplasmic reticulum (ER) parent dendrite (Vlachos et al. 2009, Korkotian Segal, 2011, Zhang 2013). SP also axon initial segment, association with cisternal organelle, another related to reticulum. Extensive research using knockout (SPKO) mice suggests that has pivotal role structural...
Abstract Dendritic spines are critical components of the neuronal synapse as they receive and transform synaptic input into a succession biochemical events regulated by calcium signaling. The spine apparatus (SA), an extension smooth endoplasmic reticulum (ER), regulates slow fast dynamics in spines. Calcium release from SA result rapid depletion ion reservoir, yet next cycle signaling requires replenishment stores. How dendritic achieve this without triggering remains unclear. Using...