A5025067741- Neuroscience and Neuropharmacology Research
- Genetics, Aging, and Longevity in Model Organisms
- Photoreceptor and optogenetics research
- Invertebrate Immune Response Mechanisms
- Autophagy in Disease and Therapy
- Lipid Membrane Structure and Behavior
- Neurobiology and Insect Physiology Research
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Mitochondrial Function and Pathology
- Ion channel regulation and function
- Toxoplasma gondii Research Studies
- Functional Brain Connectivity Studies
- Lysosomal Storage Disorders Research
- ATP Synthase and ATPases Research
- Circadian rhythm and melatonin
- RNA Research and Splicing
- Protein Tyrosine Phosphatases
- Amyotrophic Lateral Sclerosis Research
- Immune Response and Inflammation
- Insect symbiosis and bacterial influences
- S100 Proteins and Annexins
- Neuroendocrine regulation and behavior
- Genetics and Neurodevelopmental Disorders
Collège de France
2019-2025
Inserm
2019-2024
Université Paris Sciences et Lettres
2020-2024
Centre National de la Recherche Scientifique
2020-2024
King's College London
2018-2024
Centre Interdisciplinaire de Recherche en Biologie
2019-2024
Institut Curie
2023-2024
Sorbonne Université
2024
Uppsala University
2015
Ludwig Cancer Research
2015
Local translation is a conserved mechanism conferring cells the ability to quickly respond local stimuli. In brain, it has been recently reported in astrocytes, whose fine processes contact blood vessels and synapses. Yet specificity regulation of astrocyte remain unknown. We study hippocampal perisynaptic astrocytic (PAPs) show that they contain machinery for translation. Using refined immunoprecipitation technique, we characterize entire pool ribosome-bound mRNAs PAPs compare with one...
ABSTRACT Neurodevelopmental disorders, including X-linked intellectual disability, autism spectrum disorder or schizophrenia, can result from the mutation of oligophrenin-1 ( Ophn1 ), encoding a Rho-GTPase-activating protein. regulates synaptic development and function, in part via cytoskeleton reorganization, is expressed both neurons astrocytes. Despite crucial role astrocytes synapse altered neurodevelopmental their expression, specific impact astroglial deficiency on transmission...
Fast calcium transients (<10 ms) remain difficult to analyse in cellular microdomains, yet they can modulate key events such as trafficking, local ATP production by endoplasmic reticulum-mitochondria complex (ER-mitochondria complex), or spontaneous activity astrocytes. In dendritic spines receiving synaptic inputs, we show here that the presence of a spine apparatus (SA), which is an extension smooth ER, calcium-induced release (CICR) triggered at base fastest ions arriving Ryanodyne...
The fruitfly Drosophila melanogaster has been extensively used as a genetic model for the maintenance of nervous system's functions. Glial cells are utmost importance in regulating neuronal functions adult organism and progression neurological pathologies. Through microRNA-based screen glia, we uncovered essential role major glia developmental determinant, repo, fly. Here, report that Repo expression is continuously required to transcriptionally regulate highly conserved function...
Dendritic spines are critical components of neuronal synapses as they receive and transform synaptic inputs into a succession calcium-regulated biochemical events. The spine apparatus (SA), an extension smooth endoplasmic reticulum, regulates slow fast calcium dynamics in spines. Calcium release events deplete SA ion reservoir rapidly, yet the next cycle signaling requires its replenishment. How achieve this replenishment without triggering remains unclear. Using computational modeling, STED...
Despite their different origins, Drosophila glia and hemocytes are related cell populations that provide an immune function. patrol the body cavity act as macrophages outside nervous system, whereas originate from neuroepithelium scavenger population of system. hence functional orthologs vertebrate microglia, even though latter cells origin subsequently move into brain during development. Interestingly, within (glia) (hemocytes) system require same transcription factor glial deficient/glial...
Mitochondrial dysfunction activates the mitochondrial retrograde signaling pathway, resulting in large scale changes gene expression. neurons is poorly understood and whether contributes to cellular or protective unknown. We show that inhibition of Ras-ERK-ETS partially reverses transcriptional response alleviate neuronal dysfunction. have developed a novel genetic screen identify genes modify Drosophila. Knock-down one identified this screen, pathway transcription factor Aop, alleviates...
Epilepsy is a common neurological condition that arises from dysfunctional neuronal circuit control due to either acquired or innate disorders. Autophagy an essential housekeeping mechanism, which causes severe proteotoxic stress when impaired. impairment has been associated epileptogenesis through variety of molecular mechanisms. Vici Syndrome (VS) the paradigmatic congenital autophagy disorder in humans recessive variants ectopic P-granules tethering factor 5 (EPG5) gene crucial for...
Abstract Astrocytes are an indispensable part of signal processing within the mammalian brain. Thus, mode action a neuropeptide such as oxytocin (OXT) can only be fully understood considering this integral CNS. Here, we show that OXT regulates astrocytic gene expression, intracellular signaling and specific proteins both in vitro vivo. This translates into rapid regulation astroglial structural functional properties including cytoskeletal plasticity, coverage synapses gap-junction coupling....
Abstract Epilepsy is a common neurological condition that arises from dysfunctional neuronal circuit control due to either acquired or innate disorders. Autophagy an essential housekeeping mechanism, which causes severe proteotoxic stress when impaired. impairment has been associated epileptogenesis through variety of molecular mechanisms. Vici Syndrome (VS) the paradigmatic congenital autophagy disorder in humans recessive variants ectopic P-granules tethering factor 5 ( EPG5 ) gene crucial...
Genomics allows identification of genes and mutations associated with amyotrophic lateral sclerosis (ALS). Mutations in annexin A11 (ANXA11) are responsible for ~1% all familial ALS fronto-temporal dementia cases. The present study used the fruit fly,
Abstract Local translation is a conserved molecular mechanism conferring cells the ability to quickly respond local stimuli. It not only permits with complex morphology bypass somatic protein synthesis and transport, but also contributes locally establishment of functional polarity. In brain, has been extensively studied in neurons recently reported astrocytes, whose fine processes contact both blood vessels synapses. Yet specificity regulation astrocyte remain unknown. Here, we hippocampal...
Abstract Astrocytes have long been considered to be a largely homogeneous cell population. Recent studies however suggest that astrocytes are highly adapted the local neuronal circuitry. Glucose utilization in retinorecipient superior colliculus (SC) is one of highest brain. Since metabolic support neurons major function astrocytes, they could particular relevance this region and display specific features. However, little known about their interactions with multisensory brain area. We thus...
Abstract Activation of JAK/STAT signaling by IFN-γ requires partitioning IFN-γR into specific lipid nanodomains at the plasma membrane. Using as a proxy, we investigated role actin dynamics in formation and organization nanodomains, process that remains poorly understood. We identified formin Dia2/DIAPH3 RhoA -dependent regulator IFN-γ-induced signaling. Based on lipidomics probes enabling membrane imaging super resolution microscopy, demonstrate Dia2 is required for proper assembly...
<ns5:p>Invertebrate glia performs most of the key functions controlled by mammalian in nervous system and provides an ideal model for genetic studies glial functions. To study influence adult cells ageing we have performed a screen <ns5:italic>Drosophila</ns5:italic> using collection transgenic lines providing conditional expression micro-RNAs (miRNAs). Here, describe methodological algorithm to identify rank genes that are candidate be targeted miRNAs shorten lifespan when expressed glia....
<ns4:p>Invertebrate glia performs most of the key functions controlled by mammalian in nervous system and provides an ideal model for genetic studies glial functions. To study influence adult cells ageing we have performed a screen <ns4:italic>Drosophila</ns4:italic> using collection transgenic lines providing conditional expression micro-RNAs (miRNAs). Here, describe methodological algorithm to identify rank genes that are candidate be targeted miRNAs shorten lifespan when expressed glia....
Abstract Dendritic spines are critical components of the neuronal synapse as they receive and transform synaptic input into a succession biochemical events regulated by calcium signaling. The spine apparatus (SA), an extension smooth endoplasmic reticulum (ER), regulates slow fast dynamics in spines. Calcium release from SA result rapid depletion ion reservoir, yet next cycle signaling requires replenishment stores. How dendritic achieve this without triggering remains unclear. Using...