A5059095064- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Microbial infections and disease research
- Plant Virus Research Studies
- Nanoparticles: synthesis and applications
- Healthcare and Environmental Waste Management
- Monoclonal and Polyclonal Antibodies Research
- Antibiotic Resistance in Bacteria
- Plant Pathogenic Bacteria Studies
- Viral gastroenteritis research and epidemiology
- Cancer Research and Treatments
- Environmental remediation with nanomaterials
- RNA Interference and Gene Delivery
- DNA Repair Mechanisms
- Extraction and Separation Processes
- Nerve injury and regeneration
- Axon Guidance and Neuronal Signaling
- Advanced Nanomaterials in Catalysis
- Insect Resistance and Genetics
- Neurogenesis and neuroplasticity mechanisms
Texas A&M University
2013-2024
Texas A&M University System
2023
James Madison University
2013
Texas A&M University – Kingsville
2010-2012
Mitchell Institute
2012
Abstract Single-strand RNA (ssRNA) Fiersviridae phages cause host lysis with a product of single gene ( sgl for single-gene lysis; Sgl) that induces autolysis. Many different Sgls have been discovered, but the molecular targets only few identified. In this study, we used high-throughput genetic screen to uncover genome-wide suppressors diverse Sgls. addition validating known mechanisms, discovered Sgl PP7, an ssRNA phage Pseudomonas aeruginosa , MurJ, flippase responsible lipid II export,...
Although the F-specific ssRNA phage MS2 has long had paradigm status, little is known about penetration of genomic RNA (gRNA) into cell. The initially binds to F-pilus using its maturation protein (Mat), and then Mat-bound gRNA released from viral capsid somehow crosses bacterial envelope cytoplasm. To address mechanics this process, we fluorescently labeled track dynamics during infection. We discovered that infection triggers release F-pili host cells, higher multiplicity (MOI) correlates...
Acinetobacters pose a significant threat to human health, especially those with weakened immune systems. Type IV pili of acinetobacters play crucial roles in virulence and antibiotic resistance. Single-stranded RNA bacteriophages target the bacterial retractile pili, including type IV. Our study delves into interaction between Acinetobacter phage AP205 pili. Using cryo-electron microscopy, we solve structures virion an asymmetric dimer maturation proteins, native bearing distinct...
Abstract Leviviruses are bacteriophages with small single-stranded RNA genomes consisting of 3-4 genes, one which ( sgl ) encodes a protein that induces the host to undergo autolysis and liberate progeny virions. Recent meta-transcriptomic studies have uncovered thousands leviviral genomes, but most these lack an annotated , mainly due size, sequence similarity, embedded nature genes. Here, we identify genes in 244 functionally characterize them Escherichia coli . We show leviviruses readily...
ABSTRACT The L protein of the single-stranded RNA phage MS2 causes lysis Escherichia coli without inducing bacteriolytic activity or inhibiting net peptidoglycan (PG) synthesis. To find host genes required for L-mediated lysis, spontaneous Ill ( i nsensitivity to l ysis) mutants were selected as survivors expression and shown have a missense change highly conserved proline (P330Q) in C-terminal domain DnaJ. In dnaJ P330Q mutant host, is completely blocked at 30°C affecting intracellular...
ABSTRACT The study of bacteriophages infecting the model organism Bacillus subtilis has provided an abundance general knowledge and a platform for advances in biotechnology. Here, we announce annotated genome CampHawk, B. phage. CampHawk was found to be SPO1-like phage with similar gene content arrangement.
Salmonellosis, caused by Salmonella, is a leading cause of food poisoning worldwide. With the continuing rise bacterial antibiotic resistance, efforts are focused on seeking new approaches for treatment infections, namely, bacteriophage therapy. Here, we report complete genome S. Typhimurium siphophage Stitch.
Small single-stranded nucleic acid phages effect lysis by expressing a single protein, the amurin, lacking muralytic enzymatic activity. Three amurins have been shown to act like ‘protein antibiotics’ inhibiting cell-wall biosynthesis. However, L protein of canonical ssRNA phage MS2, 75 aa polypeptide, causes an unknown mechanism without affecting net peptidoglycan synthesis. To identify residues important for lytic function, randomly mutagenized alleles were generated, cloned into inducible...
Acinetobacter baumannii is an emerging multidrug-resistant nosocomial pathogen. Bacteriophages may be useful as alternative method of treatment against this and other bacteria. Here, we present the complete genome sequence A. phage Presley, N4-like podophage.
ABSTRACT Using environmentally friendly synthesis techniques, monodispersive silver nanoparticles (AgNPs) were engineered. These particles exhibited bactericidal activity against Escherichia coli under both light and dark conditions. Unlike sodium hypochlorite, which demonstrated almost immediate disinfection, AgNPs required 30 min. In contrast to however, the minimum dose of decreased as incubation time increased less than 1 part per million. The mechanism whereby inactivate microbe is...
Bacillus subtilis is a ubiquitous Gram-positive model organism. Here, we describe the complete genome of B. subtilus myophage Grass. Aside from genes encoding core proteins pertinent to life cycle phage, Grass has several interesting features, including an FtsK/SpoIIIE protein.
Salmonella enterica serovar Enteritidis is a food-borne pathogen that causes salmonellosis in the United States. Bacteriophages are emerging as viable biocontrol agents against this pathogen. Here, we present complete annotated genome sequence of T4-like myophage Marshall, which has potential phage therapy agent.
Bacillus thuringiensis is a Gram-positive, sporulating soil microbe with valuable pesticide-producing properties. The study of bacteriophages B. could provide new biotechnological tools for the use this bacterium. Here, we present complete annotated genome Spock, myophage thuringiensis, and describe its features.
The complete annotated genome sequence of Bacillus megaterium bacteriophage Slash is described here. Several key features related to morphogenesis, replication/recombination, DNA metabolism, and lysis are described. also encodes a homolog SleB, germination-specific cell wall amidase.
BigBertha is a myophage of Bacillus thuringiensis, widely used biocontrol agent that active against many insect pests plants. Here, we present the complete annotated genome BigBertha. The shares 85.9% sequence identity with cereus phage B4.
Acinetobacter baumannii is an emerging pathogen that was isolated from wounded soldiers in military treatment facilities Iraq but has since become a problem civilian hospitals. Here, we announce and describe the complete genome of KMV-like A. podophage Petty.
Bacillus megaterium is a Gram-positive, spore-forming saprophytic inhabitant of diverse environments. It reservoir for industrial chemical production and emerging as model organism studying sporulation protein localization. Here, we introduce the complete genome Page, novel podophage infecting B. megaterium.
ABSTRACT Siphophage Staley was isolated because of its ability to grow on Bacillus megaterium . Here we report the complete genome and annotation phage describe core features. Among interesting genes is one encoding an SleB germination protein.
Salmonella enterica serovar Typhimurium is a pathogenic bacterium that has been major concern for food and public safety. Phages infecting S. may prove to be useful therapeutics against this harmful bacterium. Here, we announce the complete genome of T4-like myophage Maynard describe its features.
ABSTRACT Bacillus megaterium podophage Pony was isolated from a soil sample collected in College Station, TX. Here, we report the sequencing and annotation of 39,844-bp genome phage describe major features identified.