A5058402963- Biofuel production and bioconversion
- Fungal and yeast genetics research
- Carbon and Quantum Dots Applications
- Microbial Metabolic Engineering and Bioproduction
- Advanced biosensing and bioanalysis techniques
- Enzyme Production and Characterization
- Nanoplatforms for cancer theranostics
- Nanocluster Synthesis and Applications
- Fungal Biology and Applications
- Cancer Research and Treatments
- Photodynamic Therapy Research Studies
- Calcium Carbonate Crystallization and Inhibition
- Luminescence and Fluorescent Materials
- Advanced Nanomaterials in Catalysis
- Graphene and Nanomaterials Applications
- Electrochemical sensors and biosensors
- Antimicrobial Peptides and Activities
- Lipid metabolism and biosynthesis
- Antifungal resistance and susceptibility
- Quantum Dots Synthesis And Properties
- Protein Hydrolysis and Bioactive Peptides
- Plant nutrient uptake and metabolism
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme-mediated dye degradation
- Enzyme Catalysis and Immobilization
State Key Laboratory of Digital Medical Engineering
2016-2024
Southeast University
2015-2024
Tianjin University of Science and Technology
2023
Bridge University
2020
Southeast University
2020
Beijing Institute of Technology
2014-2017
The University of Texas MD Anderson Cancer Center
2017
University of Michigan
2011-2015
Tianjin University
2009-2013
Michigan United
2013
Background and Aims The development progression of hepatocellular carcinoma (HCC) is dependent on its local microenvironment. Tumor‐associated macrophages (TAMs) are deemed a key factor for the tumor microenvironment attribute to contribute aggressiveness. However, detailed mechanism underlying pro‐metastatic effect TAMs HCC remains undefined. Approach Results present study proved that were enriched in HCC. characterized by an M2‐polarized phenotype accelerated migratory potential cells...
Isobutanol is a promising next-generation biofuel with demonstrated high yield microbial production, but the toxicity of this molecule reduces fermentation volumetric productivity and final titer. Organic solvent tolerance complex, multigenic phenotype that has been recalcitrant to rational engineering approaches. We apply experimental evolution followed by genome resequencing gene expression study elucidate genetic bases adaptation exogenous isobutanol stress.The adaptations acquired in our...
The ureolytic bacterium Sporosarcina pasteurii is well-known for its capability of microbially induced calcite precipitation (MICP), representing a great potential in constructional engineering and material applications. However, the molecular mechanism biomineralization remains unresolved, as few studies were carried out.
Carbon dots (or carbon quantum dots) are small (less than 10 nm) and luminescent nanoparticles with some form of surface passivation. As an emerging class nanomaterials, have found wide applications in medicine, bioimaging, sensing, electronic devices, catalysis. In this review, we focus on the recent advancements for sensing killing microorganisms, including bacteria, fungi, viruses. Synthesis, functionalization, a toxicity profile these presented. We also discuss underlying mechanisms...
Biofilms are deleterious in many biomedical and industrial applications prevention of their formation has been a pressing challenge. Here, carbon dots, CDs-LP that were easily synthesized from the biomass Lactobacillus plantarum by one-step hydrothermal carbonization, demonstrated to prevent biofilm E. coli. did not thwart growth coli, indicating anti-biofilm effect was due bactericidal effect. Moreover, affect animal cell AT II, showing low cytotoxicity, good safety excellent...
The decarboxylation of antimicrobial aromatic acids such as phenylacrylic acid (cinnamic acid) and ferulic by yeast requires two enzymes described decarboxylase (PAD1) (FDC). These are interest for various biotechnological applications, the production chemical feedstocks from lignin under mild conditions. However, specific role each protein in catalyzing reaction remains unknown. To examine this, we have overexpressed purified both PAD1 FDC E. coli. We demonstrate that is a flavin...
The discrimination between dead and live cells is crucial for cell viability evaluation. Carbon dots (CDs), with advantages like simple cost-effective synthesis, excellent biocompatibility, high photostability, have shown potential realizing selective live/dead staining. However, most of the developed CDs capacity usually low photoluminescence quantum yields (PLQYs) excitation wavelength-dependent fluorescence emission (which can cause overlap other fluorescent probes make dual-color...
Carbon dots (CDs), a type of small, carbon element‐based nanomaterials, have found numerous applications in many fields due to their outstanding properties like fascinating photoluminescence characteristic, great biocompatibility, easy and economical synthesis, facile functionalization. Over the last decade, huge progress achievements been made terms CDs cellular detection. However, comprehensive review focusing on this topic is still lacking. Herein, recent CDs‐mediated intracellular...
Abstract Nucleolus, which participates in many crucial cellular activities, is an ideal target for evaluating the state of a cell or organism. Here, bright red‐emissive carbon dots (termed CPCDs) with excitation‐independent/polarity‐dependent fluorescence emission are synthesized by one‐step hydrothermal reaction between congo red and p ‐phenylenediamine. The CPCDs can achieve wash‐free, real‐time, long‐term, high‐quality nucleolus imaging live cells, as well vivo two common model...
The molecular mechanism involved in tolerance and adaptation of ethanologenic Saccharomyces cerevisiae to inhibitors (such as furfural, acetic acid, phenol) represented lignocellulosic hydrolysate is still unclear. Here, (18)O-labeling-aided shotgun comparative proteome analysis was applied study the global protein expression profiles S. under conditions treatment furfural compared with furfural-free fermentation profiles. Proteins glucose and/or tricarboxylic acid cycle were upregulated...
The induction of cellulase production by insoluble carbon source cellulose was a common and efficient strategy, but has some drawbacks, such as difficult fermentation operation, substantial loss, long time, high energy-consumption, resulting in cost industry. These drawbacks can be overcome if soluble sources are utilized the inducers for production. However, until now efficiency most sources, especially lactose glucose, is still inferior to despite extensive efforts have been made either...
Imaging biofilm-encased microorganisms is challenging due to them being shielded by a sticky and strong extracellular polymeric substance matrix that blocks the penetration of dyes, but especially important for biofilm study. Here, carbon dots, namely CDs-605, were used successfully imaging microorganisms. We demonstrated such CDs-605 could be easily synthesized from L. plantarum one-step hydrothermal carbonization. The negatively charged composed C, H, O, N, P, S contained various...
The conversion of cellulose by cellulase to fermentable sugars for biomass-based products such as cellulosic biofuels, biobased fine chemicals and medicines is an environment-friendly sustainable process, making wastes profitable bringing economic benefits. Trichoderma reesei the well-known major workhorse production in industry, but low β-glucosidase activity T. leads inefficiency biomass degradation limits its industrial application. Thus, there are ongoing interests research develop...
Abstract Multidrug‐resistant bacteria (MRB) and their biofilms, both of which develop high levels drug tolerance, cause severe threats to global health. This study demonstrates that biocompatible fluorescent silicon‐containing nanodots can be a multifunctional platform for simultaneously imaging eliminating MRB biofilms. Ultrasmall epoxy group (oxirane)‐functionalized organosilica (OSiNDs) with photoluminescence quantum yield ≈31% are synthesized via simple one‐step hydrothermal treatment an...
The development of new microbicidal compounds has become a top priority due to the emergence and spread drug-resistant pathogenic microbes. In this study, blue-emitting positively charged carbon dots (CDs), called Du-CDs, were fabricated for first time utilizing natural product extract endophyte Diaporthe unshiuensis YSP3 as raw material through one-step solvothermal method, which possessed varied functional groups including amino, carboxyl, hydroxyl, sulfite groups. Interestingly, Du-CDs...
Although β-glucosidases play an important role in fungal cellulase induction and production, our current understanding does not provide a global perspective on β-glucosidase function. This work comprehensively studies all the regarding their effect production cellular distribution secretion.
Long-chain acyl-CoA reductases (ACRs) catalyze a key step in the biosynthesis of hydrocarbon waxes. As such they are attractive as components engineered metabolic pathways for 'drop in' biofuels. Most ACR enzymes integral membrane proteins, but cytosolic was recently discovered cyanobacteria. The from Synechococcus elongatus overexpressed Escherichia coli, purified and characterized. enzyme specific NADPH catalyzed reduction fatty esters to corresponding aldehydes, rather than alcohols....
Fluorescent dye staining combined with fluorescence microscopy or flow cytometry is becoming a routine way to monitor microorganism viability that necessary for food safety, antibiotic development, and human health. However, the conventional live/dead assay dyes suffer from high cost, inconvenient steps, cytotoxicity, which urgently needed overcome. Herein, cheap carbon dots, CDs-EPS605, were reported successfully assess microbial in convenient neglectable cytotoxicity. The fluorescent...
Filamentous fungi secret hydrolytic enzymes like cellulase and hemicellulase outside the cells, serving as important scavengers of plant biomass in nature workhorses enzyme industry. Unlike extensive study on mechanism production fungi, research spatiotemporal distribution secretion is lacking, retarding deeper understanding molecular behind fungal production. Recombinant Trichoderma reesei strains RBGL, RCBH, RCMC were successfully constructed from T. RUT-C30, expressing red fluorescent...