A5031960134- Genetic and phenotypic traits in livestock
- Meat and Animal Product Quality
- Cancer-related molecular mechanisms research
- Adipose Tissue and Metabolism
- Immune Cell Function and Interaction
- Ruminant Nutrition and Digestive Physiology
- Cytomegalovirus and herpesvirus research
- Viral-associated cancers and disorders
- Herpesvirus Infections and Treatments
- interferon and immune responses
- Metabolism, Diabetes, and Cancer
- Inflammasome and immune disorders
- Viral gastroenteritis research and epidemiology
- Gut microbiota and health
- Infant Nutrition and Health
- IL-33, ST2, and ILC Pathways
- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- Immune cells in cancer
- Peroxisome Proliferator-Activated Receptors
- Animal Nutrition and Physiology
- Antimicrobial Peptides and Activities
- Lipid metabolism and biosynthesis
- Reproductive System and Pregnancy
The University of Texas Southwestern Medical Center
2020-2023
Roman L. Hruska U.S. Meat Animal Research Center
2016-2018
Hastings College
2016
To better understand which genes play a role in cattle feed intake and gain, we evaluated differential expression of related to gain the liver crossbred beef steers. Based on past transcriptomics studies liver, hypothesized that metabolism regulation inflammatory response would be differentially expressed. This study used 16 animals with diverse phenotypes compare transcript abundance after 78 day ad libitum study.A total 729 were These analyzed for over-representation among biological...
Reactive oxygen species (ROS) are by-products of cellular respiration that can promote oxidative stress and damage proteins lipids. One canonical role ROS is to defend the cell against invading bacterial viral pathogens. Curiously, some viruses, including herpesviruses, thrive despite induction ROS, suggesting beneficial for virus. However, underlying mechanisms remain unclear. Here, we found impaired interferon response during murine herpesvirus infection inhibition occurred downstream...
A diverse group of antimicrobial proteins (AMPs) helps protect the mammalian intestine from varied microbial challenges. We show that small proline-rich protein 2A (SPRR2A) is an intestinal antibacterial phylogenetically unrelated to previously discovered AMPs. In this study, SPRR2A was expressed in Paneth cells and goblet selectively killed Gram-positive bacteria by disrupting their membranes. shaped microbiota composition, restricted bacterial association with surface, protected against...
Feed intake and body weight gain are economically important inputs outputs of beef production systems. The purpose this study was to discover differentially expressed genes that will be robust for feed across a large segment the cattle industry. Transcriptomic studies often suffer from issues with reproducibility cross-validation. One way improve is by integrating multiple datasets via meta-analysis. RNA sequencing (RNA-Seq) performed on longissimus dorsi muscle 80 steers (5 cohorts, each 16...
The objective of this study was to determine the association differentially expressed genes (DEG) in jejunum steers with average DMI and high or low ADG. Feed intake growth were measured a cohort 144 commercial Angus consuming finishing diet containing (on DM basis) 67.8% dry-rolled corn, 20% wet distillers grains solubles, 8% alfalfa hay, 4.2% vitamin/mineral supplement. From cohort, subset within ±0.32 SD mean for greatest (high) least (low) ADG chosen slaughter mucosa collection (n = 8...
Ruminal genes differentially expressed in crossbred beef steers from USMARC with variation gain and feed intake were identified a previous study. Several of the expression patterns differing between animals high gain-low low gain-high evaluated separate, unrelated population Angus × Hereford University Wyoming that was classified to differ residual (RFI). Of 17 tested, two by RFI class animals. These included NAD(P)H dehydrogenase, quinone 1 (NQO1; P = 0.0009) regulator G-protein signaling 5...
Even though gammaherpesvirus and parasitic infections are endemic in parts of the world, there is a lack understanding about outcome coinfection. In humans, coinfections usually occur sequentially, with fluctuating order timing different hosts. However, experimental studies mice generally do not address variables coinfections. We sought to examine variable coinfection system gammaherpesvirus-helminth Our previous work demonstrated that infection intestinal parasite, Heligmosomoides polygyrus...
Herpesviruses establish lifelong quiescent infections in specific cells the body and reactivate to produce infectious virus only when precise signals induce them do so. The that herpesvirus reactivation are often studied one particular cell type infected with virus.
A previous study infusing butyrate into the abomasum of sheep produced increased oxygen, glucose, glutamate, and glutamine uptake by portal-drained viscera. These changes were thought to be partially due increases in glycolysis cell proliferation. The purpose this was evaluate duodenum transcriptome control butyrate-treated lambs determine whether genes involved these pathways altered. Polled Dorset ( = 9) received a pulse dose either (10 mg/kg BW) or an equal volume buffered saline solution...
The purpose of this study was to identify genes differentially expressed in the muscle beef cattle associated with gain and intake regardless breed origin season or year study. Crossbred animals 19 different breeds variation BW feed were selected from fall spring seasons over 3 yr reduce influence gene expression that either specific related environment cohort. Ribonucleic acid sequencing performed on longissimus dorsi 80 steers (5 cohorts, each 16 animals). Each cohort displayed a clear...
The purpose of this study was to identify genes associated with gain and intake in the liver beef cattle. uses 25% body's energy, while constituting less than 2% total body weight. It is likely that plays a key role due its high energy utilization metabolism. Yearling, crossbred-steers were put on 78-d feed trial. Average daily average calculated at end 16 steers greatest deviation from bivariate mean selected for slaughter: 4 gain-high (HG-HI); gain-low (HG-LI), low (LG-LI); (LG-HI). Liver...
High-throughput sequencing is often used for studies of the transcriptome, particularly comparisons between experimental conditions. Due to costs, a limited number biological replicates are typically considered in such experiments, leading low detection power differential expression. Moreover, validation transcriptomic data likely suffer from poor reproducibility study due large amount variation sources, as breed and season. The major aim this was identify genes differentially expressed...
Journal Article Effect of abomasal butyrate infusion on gene expression in the duodenum lambs Get access A. P. Foote, Foote *USDA, ARS, U.S. Meat Animal Research Center, Clay NE 68933-0166 2Corresponding author: Andrew.Foote@ars.usda.gov Search for other works by this author on: Oxford Academic PubMed Google Scholar C. M. Zarek, Zarek L.A. Kuehn, Kuehn H. Cunningham, Cunningham †Department Science, University Wyoming, Laramie 82071 K. Cammack, Cammack Freetly, Freetly Lindholm-Perry Volume...
Abstract Herpesviruses are a component of our virome, and infect virtually everyone. They establish chronic latent infection that is punctuated with periods viral reactivation. Reactivation thought to drive herpesvirus pathologies, including lymphomas tumors. However, the signals promote reactivation poorly understood. We previously showed parasite co-infection could induce through induction cytokines IL-4 IL-13, activation Stat6. Stat6 directly binds promoter induces virus reactivation,...
Abstract A vast majority of people are infected with a herpesvirus. These viruses double stranded DNA that avoid eradication by the immune system becoming latent. Certain signals, such as repression, can lead to reactivation virus. Reactivation gamma herpesviruses is associated pathologies, lymphomas and tumors. Past studies have shown infection helminths, extracellular parasitic worms, during herpesvirus latency cause reactivation. Helminths known secrete immunomodulatory factors suppress...