A5066058761- Immune Cell Function and Interaction
- Gut microbiota and health
- Malaria Research and Control
- Complement system in diseases
- Inflammation biomarkers and pathways
- Mycobacterium research and diagnosis
- Curcumin's Biomedical Applications
- Immune cells in cancer
- Clostridium difficile and Clostridium perfringens research
- Neuroinflammation and Neurodegeneration Mechanisms
- Psoriasis: Treatment and Pathogenesis
- Drug-Induced Hepatotoxicity and Protection
- Retinoids in leukemia and cellular processes
- Reproductive System and Pregnancy
- Dietary Effects on Health
- Phytochemistry and Bioactivity Studies
- Antioxidant Activity and Oxidative Stress
- Pancreatitis Pathology and Treatment
- Pharmacological Effects of Natural Compounds
- Phagocytosis and Immune Regulation
- Cancer-related molecular mechanisms research
- Computational Drug Discovery Methods
- Calpain Protease Function and Regulation
- Probiotics and Fermented Foods
- Cytomegalovirus and herpesvirus research
Southwestern University
2024
The University of Texas Southwestern Medical Center
2019-2023
Indian Institute of Science Bangalore
2012-2017
Pondicherry University
2010
The intestinal microbiota regulates mammalian lipid absorption, metabolism, and storage. We report that the reprograms metabolism in mice by repressing expression of long noncoding RNA (lncRNA) Snhg9 (small nucleolar host gene 9) small epithelial cells. suppressed activity peroxisome proliferator–activated receptor γ (PPARγ)—a central regulator metabolism—by dissociating PPARγ inhibitor sirtuin 1 from cell cycle apoptosis protein 2 (CCAR2). Forced epithelium conventional impaired reduced...
Abstract Curcumin has many pharmacological activities despite its poor bioavailability and in vivo stability. Here, we show that a nanoformulated curcumin (PLGA-curcumin) better therapeutic index than native preventing the onset of neurological symptoms delaying death mice experimental cerebral malaria. Oral PLGA-curcumin was at least as effective 15-fold lower concentration breakdown blood-brain barrier inhibition brain mRNAs for inflammatory cytokines, chemokine receptor CXCR3 ligand...
Peristaltic movement of the intestine propels food down length gastrointestinal tract to promote nutrient absorption. Interactions between intestinal macrophages and enteric nervous system regulate motility, yet we have an incomplete understanding molecular mediators this crosstalk. Here, identify complement component 1q (C1q) as a macrophage product that regulates gut motility. Macrophages were predominant source C1q in mouse most extraintestinal tissues. Although mediates...
Earlier studies in this laboratory have shown the potential of artemisinin-curcumin combination therapy experimental malaria. In a parasite recrudescence model mice infected with Plasmodium berghei (ANKA), single dose alpha,beta-arteether (ART) three oral doses curcumin prevented recrudescence, providing almost 95% protection. The parasites were completely cleared blood ART-alone (AE) or ART+curcumin (AC) treatments short-term, although clearance was faster latter case involving increased...
Vitamin A is a dietary component that essential for the development of intestinal immunity. absorbed and converted to its bioactive derivatives retinol retinoic acid by epithelium, yet little known about how epithelial cells regulate vitamin A-dependent Here we show cell expression transcription factor receptor β (RARβ) Epithelial RARβ activated serum amyloid (SAA) proteins binding directly Saa promoters. In accordance with role SAAs in regulating Th17 effector function, promoted IL-17...
Bead-beating within a DNA extraction protocol is critical for complete microbial cell lysis and accurate assessment of the abundance composition microbiome. While impact bead-beating on recovery OTUs at phylum class level have been studied, its influence species-level microbiome not clear. Recent advances in sequencing technology has allowed resolution using full length 16S rRNA gene instead smaller amplicons that only capture few hypervariable regions gene. We sequenced v3-v4 region as well...
Abstract Malaria afflicts around 200 million people annually, with a mortality number close to 600,000. The rate in Human Cerebral (HCM) is unacceptably high (15–20%), despite the availability of artemisinin-based therapy. An effective adjunct therapy urgently needed. Experimental (ECM) mice manifests many neurological features HCM. Migration T cells and parasite-infected RBCs (pRBCs) into brain are both necessary precipitate disease. We have been able simultaneously target these parameters...
The relationship between chronic alcohol consumption and various hepatic lesions are grouped under the term alcoholic liver disease. This is an extremely common disease with a high mortality. Alcoholics, along alcohol, consume fat diet susceptible to permanent damage. current treatment modalities inadequate need for effective without side-effects increasing. present work tested protective role of Phyllanthus niruri aqueous leaf extract on heated sunflower oil-induced hyperlipidemia. Male...
Even though gammaherpesvirus and parasitic infections are endemic in parts of the world, there is a lack understanding about outcome coinfection. In humans, coinfections usually occur sequentially, with fluctuating order timing different hosts. However, experimental studies mice generally do not address variables coinfections. We sought to examine variable coinfection system gammaherpesvirus-helminth Our previous work demonstrated that infection intestinal parasite, Heligmosomoides polygyrus...
Abstract DNA extraction methods play an important role in the acquisition of accurate and reproducible 16S sequencing data microbiome studies. In this study, we assessed impact bead-beating intensity during on recovery mouse human stool. We observed a higher yield, better integrity, Shannon’s entropy Simpson’s index samples beaten for 4 9 minutes as compared to unbeaten samples. showed that bead beating has statistically-significant (p<0.05) many clinically relevant microbes live gut,...
ABSTRACT Peristaltic movement of the intestine propels food down length gastrointestinal tract to promote nutrient absorption. Interactions between intestinal macrophages and enteric nervous system regulate motility, yet we have an incomplete understanding molecular mediators this crosstalk. Here identify complement component 1q (C1q) as a macrophage product that regulates gut motility. Macrophages were predominant source C1q in mouse most extraintestinal tissues. Although mediates...
Abstract Small intestinal epithelial cells (sIECs) form a critical barrier essential for nutrient absorption and host defense. sIECs undergo continuous renewal to maintain their integrity. Phagocytes, such as macrophages, facilitate the clearance of apoptotic that enter subepithelial tissues. However, little is known about how sIEC-phagocyte crosstalk maintains Our study reveals synchrony between sIEC death accumulation specific subset macrophages engaged in engulfing dying cells. Notably,...
Abstract Vitamin A is a dietary component that essential for the development of intestinal immunity. absorbed and converted to its bioactive derivatives retinol retinoic acid by epithelium, yet little known about how epithelial cells regulate vitamin A-dependent Here we show cell expression transcription factor receptor β (RARβ) Epithelial RARβ activated serum amyloid (SAA) proteins binding directly Saa promoters. In accordance with role SAAs in regulating Th17 effector function, promoted...
Abstract In addition to a range of homeostatic functions, resident macrophages are essential for immune surveillance in tissues. Therefore, anything that alters the phenotype or function these cells potentially impacts their response infectious challenges. Parasite infections cause proliferation large peritoneal (LPMs), which cavity. However, functional consequences LPM expansion on control secondary challenge is unknown. Using coinfection model with intestinal parasite Heligmosomoides...
Abstract Intestinal Tim4+ CD4+ macrophages are a distinctive macrophage subset that express Tim4, receptor for phosphatidylserine on dying apoptotic cells, Unlike other subsets, they do not depend blood monocytes their turnover, instead self-maintained in the small intestine. The signal(s) responsible self-maintenance and function of is known. We have discovered maintenance gut resident population depends dietary vitamin A its derivative retinoic acid (RA). Retinoic receptors, which direct...