A5102732172- Systemic Lupus Erythematosus Research
- Gut microbiota and health
- Cytokine Signaling Pathways and Interactions
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Rabies epidemiology and control
- Poxvirus research and outbreaks
- Monoclonal and Polyclonal Antibodies Research
- Clostridium difficile and Clostridium perfringens research
- Mast cells and histamine
- Viral gastroenteritis research and epidemiology
- Immunodeficiency and Autoimmune Disorders
- RNA regulation and disease
- Prostate Cancer Treatment and Research
- Microbial infections and disease research
- RNA Research and Splicing
- SARS-CoV-2 and COVID-19 Research
- Dermatology and Skin Diseases
- Cancer Genomics and Diagnostics
- Streptococcal Infections and Treatments
- Cancer-related molecular mechanisms research
- Complement system in diseases
- Mycobacterium research and diagnosis
- Genetic Syndromes and Imprinting
- Cancer Immunotherapy and Biomarkers
The University of Texas Southwestern Medical Center
2016-2024
Southwestern Medical Center
2019-2022
Hospital Virgen de la Luz
2022
Hospital Universitario Virgen de las Nieves
2021
Hospital Clínico Universitario Virgen de la Victoria
2012
University of Miami
2001
Emerging evidence indicates that various cancers can gain resistance to targeted therapies by acquiring lineage plasticity. Although genomic and transcriptomic aberrations correlate with plasticity, the molecular mechanisms enabling acquisition of plasticity have not been fully elucidated. We reveal Janus kinase (JAK)-signal transducer activator transcription (STAT) signaling is a crucial executor in promoting plasticity-driven androgen receptor (AR)-targeted therapy prostate cancer....
Highlights•Depletion of Clostridia species is associated with graft-versus-host disease in pediatric stem cell transplantation patients•Exposure to antianaerobic antibiotics (eg, clindamycin) disease•Shotgun sequencing identified 4 depleted patients•Clindamycin depletes and exacerbates mice•Clostridia supplementation mitigates miceAbstractAdult (SCT) patients graft-versus-host-disease (GVHD) exhibit significant disruptions gut microbial communities. These changes are higher overall mortality...
Targeted sequencing of sixteen SLE risk loci among 1349 Caucasian cases and controls produced a comprehensive dataset the variations causing susceptibility to systemic lupus erythematosus (SLE). Two independent disease association signals in HLA-D region identified two regulatory regions containing 3562 polymorphisms that modified thirty-seven transcription factor binding sites. These extensive functional are new potent facet HLA polymorphism. Variations modifying consensus motifs IRF4 CTCF...
Myeloid lineage cells use TLRs to recognize and respond diverse microbial ligands. Although unique transcription factors dictate the outcome of specific TLR signaling, whether lineage-specific differences exist further modulate quality TLR-induced inflammation remains unclear. Comprehensive analysis global gene in human monocytes, monocyte-derived macrophages, dendritic stimulated with various ligands identifies multiple lineage-specific, TLR-responsive programs. Monocytes are...
The effect of the familial hypertrophic cardiomyopathy mutations, A13T, F18L, E22K, R58Q, and P95A, found in regulatory light chains human cardiac myosin has been investigated. results demonstrate that E22K located immediate extension helices flanking chain Ca2+ binding site, had dramatically altered properties. K Cavalue for was decreased by ∼17-fold compared with wild-type chain, R58Q mutant did not bind Ca2+. Interestingly, to restored upon phosphorylation, whereas could be...
Bead-beating within a DNA extraction protocol is critical for complete microbial cell lysis and accurate assessment of the abundance composition microbiome. While impact bead-beating on recovery OTUs at phylum class level have been studied, its influence species-level microbiome not clear. Recent advances in sequencing technology has allowed resolution using full length 16S rRNA gene instead smaller amplicons that only capture few hypervariable regions gene. We sequenced v3-v4 region as well...
The potential for microRNAs (miRNAs) to regulate gene expression remains incompletely understood. DROSHA initiates the biogenesis of miRNAs while variants Argonaute (AGO) and trinucleotide repeat containing six (TNRC6) family proteins form complexes with facilitate RNA recognition regulation. Here we investigate fate in absence these critical RNAi protein factors. Knockout
High viral transmission in the COVID-19 pandemic has enabled SARS-CoV-2 to acquire new mutations that may impact genome sequencing methods. The ARTIC.v3 primer pool amplifies short amplicons a multiplex-PCR reaction is one of most widely used methods for genome. We observed some genomic intervals are poorly captured with ARTIC primers. To improve coverage and variant detection across these intervals, we designed long amplicon primers evaluated performance (ARTIC) plus (MRL) approach....
Abstract Background Systemic lupus erythematosus (SLE) is a clinically heterogeneous autoimmune disease characterized by the development of anti-nuclear antibodies. Susceptibility to SLE multifactorial, with combination genetic and environmental risk factors contributing development. Like other polygenic diseases, significant proportion estimated heritability not accounted for common alleles analyzed SNP array-based GWASs. Death-associated protein 1 (DAP1) was implicated as candidate gene in...
Type 2 diabetes (T2D) is a rising global health problem mainly caused by obesity and sedentary lifestyle. In healthy individuals, white adipose tissue (WAT) has relevant homeostatic role in glucose metabolism, energy storage, endocrine signaling. Mast cells contribute to these functions promoting WAT angiogenesis adipogenesis. patients with T2D, inflammation dramatically impacts functioning, which results the recruitment of several leukocytes, including monocytes, that enhance this...
Although significant progress has been achieved in elucidating the genetic architecture of systemic lupus erythematosus (SLE), identifying genes underlying pathogenesis challenging. The NZM2410-derived susceptibility Sle3 locus is associated with T cell hyperactivity and activated myeloid cells. However, candidate these phenotypes have not identified. Here, we narrow to a smaller genomic segment (Sle3k) show that mice carrying Sle3k Sle1 loci developed nephritis. We identify Klf13 as primary...
The paradigm of mast cells in type 2 diabetes is changing. Although they were first considered deleterious inflammatory cells, now seem to be important players driving adipose tissue homeostasis. Here we have employed a flow cytometry-based approach for measuring the surface expression 4 proteins (CD45, CD117, CD203c, and FcϵRI) on omental (o-WAT) subcutaneous white (s-WAT) cohort 96 patients with morbid obesity. was split into three groups: non-T2D, pre-T2D, T2D. Noteworthy, T2D mild...
Abstract DNA extraction methods play an important role in the acquisition of accurate and reproducible 16S sequencing data microbiome studies. In this study, we assessed impact bead-beating intensity during on recovery mouse human stool. We observed a higher yield, better integrity, Shannon’s entropy Simpson’s index samples beaten for 4 9 minutes as compared to unbeaten samples. showed that bead beating has statistically-significant (p<0.05) many clinically relevant microbes live gut,...
Abstract Lipids synthesized on the skin are critical to antimicrobial barrier. Skin lipids also facilitate survival of lipophilic commensals in an otherwise dry and acidic ecological landscape. Thus, skin‐specific stearoyl‐coenzyme A desaturase 1 knockout mice ( Scd1 ΔK14 ) with sebocyte atrophy decreased synthesis monounsaturated fatty acids, triglycerides wax diesters have dry, inflamed skin. Here, we used 16S rRNA (V1‐V2 V1‐V9) internal transcribed spacer (ITS1) amplicon sequencing...
<title>Abstract</title> Background. Young children have frequent viral/bacterial infections and antibiotic exposures. Whether these events promote translocation of bacterial factors from oral cavity into the bloodstream remains poorly studied. Method. We performed 16S sequencing immune profiling blood in 210 healthy 1–2-year age range. Observed DNA signatures were analyzed context host cytokine antibody responses. Results. About 12% toddlers showed a signature related to commensals...
Abstract Emerging evidence indicates that various cancers can gain resistance to targeted therapies by acquiring lineage plasticity. Although genomic and transcriptomic aberrations correlate with plasticity-driven resistance, the molecular mechanisms of plasticity have not been fully elucidated. Through integrated single cell RNA-Seq (scRNA-Seq) analysis more than 80,000 cells, we reveal for first time Janus kinase (JAK)-signal transducer activator transcription (STAT) signaling is a crucial...
<h3>Background</h3> The antibody specificities of an infant progressively form in response to infections, environmental exposures, and vaccinations. While many adults develop antibodies self-antigens, it remains unknown if these are present infants toddlers. <h3>Methods</h3> Serum, peripheral blood, clinical data have been collected from 102 healthy children (1–2 year age). ANA titers were measured by QuantiaLite ELISA (Inova) reactivity 125 diverse autoantigens tested autoantibody array....
The aim of this study was to determine changes in the liver biochemical profile alpacas with presence F. hepatica eggs. We used serum and feces samples 35 male alpaca between 2-5 years old, 18 were positives 17 negatives coproparasitologic analysis for Differences are showed (p<0.05) values ALT, GGT direct bilirubin y animals. concluded that there is relationship eggs high levels profile, maybe due alterations hepatic parenchyma by chronic infection alpacas.
<h3>Background</h3> Systemic lupus erythematosus (SLE) is characterized by the presence of multiple autoantibodies as well clinical evidence multi-system immune-mediated pathology. Individuals who develop SLE typically demonstrate serological autoimmunity for several years prior to onset disease. Measuring anti-nuclear antibodies (ANA) a common test humoral autoimmunity. It sensitive (95+%) SLE, but not specific up 25% healthy controls (HC) will have measurable ANA. While very few ANA +HC...
Abstract The Toll-like receptors (TLRs) of the innate immune system play a key role in recognition pathogens. TLRs signal through recruitment specific adaptor molecules, leading to activation transcription factors, such as AP-1, NF-κB and interferon regulatory factors (IRFs). Although are mainly expressed cells, systematic comparison cells programs induced by individual is not yet available. Here, we used whole transcriptome sequencing evaluate global changes gene expression on monocytes,...