A5017276343- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Respiratory viral infections research
- Immune Cell Function and Interaction
- Viral gastroenteritis research and epidemiology
- Genomics and Phylogenetic Studies
- Influenza Virus Research Studies
- Heat Transfer and Optimization
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA modifications and cancer
- vaccines and immunoinformatics approaches
- Surface Modification and Superhydrophobicity
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- RNA Research and Splicing
- Ferroptosis and cancer prognosis
- Chromosomal and Genetic Variations
- RNA Interference and Gene Delivery
- Cancer Immunotherapy and Biomarkers
- Salmonella and Campylobacter epidemiology
- COVID-19 epidemiological studies
- Solar-Powered Water Purification Methods
- RNA regulation and disease
- Gene expression and cancer classification
Shandong Provincial QianFoShan Hospital
2024
Nanchang University
2024
Baylor College of Medicine
2024
Zhejiang University
2020-2024
The University of Texas Southwestern Medical Center
2022-2024
Shanghai East Hospital
2024
Shandong University
2024
Baylor Genetics
2024
Chang'an University
2024
Southwestern Medical Center
2023
Miniature inverted-repeat transposable elements (MITEs) are a special type of Class 2 nonautonomous element (TE) that abundant in the non-coding regions genes many plant and animal species.The accurate identification MITEs has been challenge for existing programs because they lack coding sequences and, as such, evolve very rapidly.Because their importance to gene genome evolution, we developed MITE-Hunter, program pipeline can identify well other small TEs from genomic DNA data sets.The...
Although rapid progress has been made in computational approaches for prioritizing cancer driver genes, research is far from achieving the ultimate goal of discovering a complete catalog genes truly associated with cancer. Driver gene lists predicted these tools lack consistency and are prone to false positives. Here, we developed an approach (DriverML) integrating Rao's score test supervised machine learning identify genes. The weight parameters statistics quantified functional impacts...
Glucokinase (GCK) serves as the pancreatic glucose sensor. Heterozygous inactivating GCK mutations cause hyperglycemia, whereas activating hypoglycemia. We studied V62M mutation identified in two families and co-segregating with hyperglycemia to understand how this resulted reduced function. Structural modeling locates close five naturally occurring allosteric activator site of enzyme. Recombinant glutathionyl S-transferase-V62M is paradoxically activated rather than inactivated due a...
Parenteral administration of killed/inactivated swine influenza A virus (SwIAV) vaccine in weaned piglets provides variable levels immunity due to the presence preexisting specific maternal derived antibodies (MDA). To overcome effect MDA on SwIAV piglets, we developed an intranasal deliverable killed antigen (KAg) encapsulated chitosan nanoparticles called chitosan-based NPs encapsulating KAg (CS NPs-KAg) vaccine. Further, target candidate dendritic cells and macrophages which express...
Background: Aberrant DNA methylation occurs commonly during carcinogenesis and is of clinical value in human cancers. However, knowledge the impact changes on lung progression remains limited. Methods: Genome-wide profiles were surveyed 18 pairs tumors adjacent normal tissues from non-small cell cancer (NSCLC) patients using Reduced Representation Bisulfite Sequencing (RRBS). An integrated epigenomic-transcriptomic landscape was depicted multi-omics data integration method. Results: We...
Abstract T cells are integral components of the adaptive immune system, and their responses mediated by unique T-cell receptors (TCR) that recognize specific antigens from a variety biological contexts. As result, analyzing repertoire offers better understanding diseases like cancer. Next-generation sequencing technologies have greatly enabled high-throughput analysis TCR repertoire. On basis our extensive experience in field past decade, we provide an overview sequencing, initial library...
Comprehending the mechanism behind human diseases with an established heritable component represents forefront of personalized medicine. Nevertheless, numerous medically important genes are inaccurately represented in short-read sequencing data analysis due to their complexity and repetitiveness or so-called 'dark regions' genome. The advent PacBio as a long-read platform has provided new insights, yet HiFi whole-genome (WGS) cost remains frequently prohibitive. We introduce targeted...
Wuhan to Wenzhou from January 10 23
The potential for microRNAs (miRNAs) to regulate gene expression remains incompletely understood. DROSHA initiates the biogenesis of miRNAs while variants Argonaute (AGO) and trinucleotide repeat containing six (TNRC6) family proteins form complexes with facilitate RNA recognition regulation. Here we investigate fate in absence these critical RNAi protein factors. Knockout
Argonaute protein is associated with post-transcriptional control of cytoplasmic gene expression through miRNA-induced silencing complexes (miRISC). Specific cellular and environmental conditions can trigger AGO to accumulate in the nucleus. Localization central understanding miRNA action, yet consequences being nucleus are undefined. We show nuclear enrichment AGO2 HCT116 cells grown two-dimensional culture high density, three-dimensional tumor spheroid culture, human colon tumors. The...
The development of antifungal drugs is an urgent task, but it has proven to be difficult due many similarities between fungal and animal cells. Here, we characterized the urease system in A. fumigatus , which depends on nickel for activity.
Intranasal vaccination elicits secretory IgA (SIgA) antibodies in the airways, which is required for cross-protection against influenza. To enhance breadth of immunity induced by a killed swine influenza virus antigen (KAg) or conserved T cell and B peptides, we adsorbed antigens together with TLR3 agonist poly(I:C) electrostatically onto cationic alpha-D-glucan nanoparticles (Nano-11) resulting Nano-11-KAg-poly(I:C) Nano-11-peptides-poly(I:C) vaccines. In vitro, increased TNF-α IL-1ß...
As essential primary producers, cyanobacteria play a major role in global carbon and nitrogen cycles. Though the influence of nanoplastics on metabolism is well-studied, little known about how affect their metabolism, especially under environmentally relevant concentrations. Here, we show that forms regulated growth inhibition, consumption, synthesis release microcystin (MC) Microcystis aeruginosa exposed to 10 μg/mL amino-modified polystyrene (PS-NH2) with particle size 50 nm concentrations...
The rapid development of next-generation sequencing technology provides an opportunity to study genome-wide DNA methylation at single-base resolution. However, depletion unmethylated cytosines brings challenges for aligning bisulfite-converted reads a large reference. Software tools have not yet been comprehensively evaluated, especially the widely used reduced representation bisulfite (RRBS) that involves enrichment CpG islands (CGIs).We specially developed simulator, RRBSsim, benchmarking...
Salmonella enterica serovar Enteritidis (S. Enteritidis, SE) infection in broilers causes a huge economic loss and public health risk. We previously demonstrated that orally delivered chitosan based (CS) subunit nanoparticle (NP) vaccine containing immunogenic outer membrane proteins (OMP) flagellin (FLA) of SE [CS-NP(OMP+FLA)] induces immune response broilers. The objective this study was to evaluate the dose- age-dependent efficacy CS-NP(OMP+FLA) Three-day old birds were vaccinated not...
Chromosome identification is critical for cytogenetic research and will accelerate studies on genetic variation breeding, especially those species with relatively little sequence information. So far, no reliable cytological landmarks have been developed to distinguish individual chromosomes in melon. In this study, using FISH (fluorescence situ hybridization) combined comparative genome information, we selected 21 cucumber fosmids anchored by SSR markers as chromosome-specific melon...
We have previously described purification and characterization of a nuclear protein, TREF, which interacts specifically with the transcriptional control element, TRA, human transferrin receptor (TR) gene. In this report we show that TREF can be separated into two functionally distinct DNA-binding activities. The first activity (TRAC) is highly specific for 8-bp element TRA related Escherichia coli cAMP binding site. This motif homologous to phorbol 12-tetradecanoate 13-acetate-...
Iron overload has been found in the lungs of patients with idiopathic pulmonary fibrosis (IPF) and is thought to be involved disease progression; however, underlying mechanism complex not yet fully understood. We sought assess vitro role iron progression lung epithelial cells, examine possible regulation IPF.Erastin was used establish a cell model accumulation mouse line 12 (MLE-12). A Cell Counting Kit-8 assay annexin V staining were applied measure viability apoptosis, quantitative...
We designed the killed swine influenza A virus (SwIAV) H1N2 antigen (KAg) with polyriboinosinic:polyribocytidylic acid [(Poly(I:C)] adsorbed corn-derived Nano-11 particle based nanovaccine called Nano-11-KAg+Poly(I:C), and evaluated its immune correlates in maternally derived antibody (MDA)-positive pigs against a heterologous H1N1 SwIAV infection. Immunologically, tracheobronchial lymph nodes (TBLN) detected enhanced H1N2-specific cytotoxic T-lymphocytes (CTLs) Nano-11-KAg+Poly(I:C)...
The prevention and control measures adopted by various countries have different effects in the face of COVID-19. performance some socialist capitalist were compared from three aspects, including proportion confirmed cases deaths to national population, economic recovery production living, social satisfaction international evaluation. overall listed was better than that countries. China's performance, forming a unique Chinese model epidemic control, other successful experience can be mainly...
Using CRISPR-Cas9 technology and a microhomology-mediated end-joining repair system, we substituted genes of the gliotoxin pathway in Aspergillus fumigatus with responsible for chetomin biosynthesis from Chaetomium cochliodes, leading to production three new epipolythiodioxopiperazines (ETPs). This work represents first successful endeavor produce ETPs non-native host. Additionally, simultaneous disruption five single transformation marks most extensive gene knockout event filamentous fungi date.