A5043796523- Influenza Virus Research Studies
- Respiratory viral infections research
- interferon and immune responses
- Immunotherapy and Immune Responses
- Immune cells in cancer
- vaccines and immunoinformatics approaches
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Immune Response and Inflammation
- Cancer, Stress, Anesthesia, and Immune Response
- Tryptophan and brain disorders
- Virus-based gene therapy research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Toxoplasma gondii Research Studies
- Virology and Viral Diseases
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Immunodeficiency and Autoimmune Disorders
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer-related gene regulation
- Animal Disease Management and Epidemiology
- Autophagy in Disease and Therapy
- Spectroscopy Techniques in Biomedical and Chemical Research
- Salmonella and Campylobacter epidemiology
- Platelet Disorders and Treatments
The Ohio State University
2020-2024
Georgetown University
2022-2024
University of Maryland, College Park
2023
Georgetown University Medical Center
2022
Augusta University Health
2022
College of Wooster
2021
Centre de Recherche des Cordeliers
2013-2020
Sorbonne Université
2013-2020
Institut National de Recherche en Santé Publique
2020
Université Paris Cité
2013-2019
Parenteral administration of killed/inactivated swine influenza A virus (SwIAV) vaccine in weaned piglets provides variable levels immunity due to the presence preexisting specific maternal derived antibodies (MDA). To overcome effect MDA on SwIAV piglets, we developed an intranasal deliverable killed antigen (KAg) encapsulated chitosan nanoparticles called chitosan-based NPs encapsulating KAg (CS NPs-KAg) vaccine. Further, target candidate dendritic cells and macrophages which express...
Abstract Autophagy plays an important role in the regulation of autoimmune and autoinflammatory responses immune cells. Defective autophagy process is associated with various inflammatory diseases. Moreover, many these diseases, therapeutic use normal immunoglobulin G or intravenous (IVIG), a pooled IgG preparation, well documented. Therefore, we explored if IVIG immunotherapy exerts benefits via induction Here show that induces peripheral blood mononuclear cells (PBMCs). Further dissection...
Abstract Background Unlike the injectable vaccines, intranasal lipid nanoparticle (NP)-based adjuvanted vaccine is promising to protect against local infection and viral transmission. Infection of ferrets with SARS-CoV-2 results in typical respiratory disease pathology akin humans, suggesting that ferret model may be ideal for studies. Results We developed subunit containing both Spike receptor binding domain (S-RBD) Nucleocapsid (N) proteins (NP-COVID-Proteins) or their mRNA (NP-COVID-mRNA)...
Toxoplasma gondii pathogenesis includes the invasion of host cells by extracellular parasites, replication intracellular tachyzoites, and differentiation to a latent bradyzoite stage. We present analysis seven novel T. insertional mutants that do not undergo normal bradyzoites. Microarray quantification variation in genome-wide RNA levels for each parasite line times after induction allowed us describe states process, analyze mutant lines context these states, identify genes may have roles...
Intranasal vaccination elicits secretory IgA (SIgA) antibodies in the airways, which is required for cross-protection against influenza. To enhance breadth of immunity induced by a killed swine influenza virus antigen (KAg) or conserved T cell and B peptides, we adsorbed antigens together with TLR3 agonist poly(I:C) electrostatically onto cationic alpha-D-glucan nanoparticles (Nano-11) resulting Nano-11-KAg-poly(I:C) Nano-11-peptides-poly(I:C) vaccines. In vitro, increased TNF-α IL-1ß...
Abstract The immunosuppressive tumor microenvironment (TME) does not allow generation and expansion of antitumor effector cells. One the potent factors present in TME is indoleamine-pyrrole 2,3-dioxygenase (IDO) enzyme, produced mainly by cancer cells suppressive immune myeloid origin. In fact, IDO+ myeloid-derived suppressor (MDSC) dendritic (DC) tend to be more than their IDO− counterparts. Hence, therapeutic approaches that would target TME, while sparing antigen-presenting functions...
Influenza A viruses (IAV-S) belonging to the H1 subtype are endemic in swine worldwide. Antigenic drift and antigenic shift lead a substantial diversity circulating IAV-S strains. As result, most commonly used vaccines based on whole inactivated (WIVs) provide low protection against divergent strains due mismatch between vaccine virus strain one. Here, consensus coding sequence of full-length HA from was generated silico after alignment sequences isolates obtained public databases delivered...
Salmonella enterica serovar Enteritidis (S. Enteritidis, SE) infection in broilers causes a huge economic loss and public health risk. We previously demonstrated that orally delivered chitosan based (CS) subunit nanoparticle (NP) vaccine containing immunogenic outer membrane proteins (OMP) flagellin (FLA) of SE [CS-NP(OMP+FLA)] induces immune response broilers. The objective this study was to evaluate the dose- age-dependent efficacy CS-NP(OMP+FLA) Three-day old birds were vaccinated not...
Swine influenza A viruses (SwIAVs) pose an economic and pandemic threat, development of novel effective vaccines is critical significance. We evaluated the performance split swine virus (SwIAV) H1N2 antigens with a plant-derived nanoparticle adjuvant alone (Nano-11) [Nano11-SwIAV] or in combination synthetic stimulator interferon genes (STING) agonist ADU-S100 (NanoS100-SwIAV). Specific pathogen free (SPF) pigs were vaccinated twice via intramuscular (IM) intradermal (ID) routes challenged...
Controlling Salmonella enterica serovar Enteritidis (SE) infection in broilers is a huge challenge. In this study, our objective was to improve the efficacy of chitosan nanoparticle (CS)-based subunit vaccine for SE, containing immunogenic outer membrane proteins (OMP) and flagellin (FLA), called CS(OMP+FLA) vaccine, by surface conjugating it with mannose target dendritic cells, comparing immune responses commercial live broilers. The CS(OMP+FLA)-based vaccines were administered orally at...
The impact of obesity on the human microbiota, immune maturation, and influenza virus infection has not been yet established in natural host animal models influenza. In this study, gnotobiotic (Gn) pigs were colonized with fecal microbiota (HFM) obese (oHFM) or healthy lean (hHFM) children infected at different periods (2-, 3-, 5-weeks post-transplantation) using a zoonotic strain. oHFM characterized by lower levels Firmicutes (Lactococcus, Lactobacillus, Turicibacter, Streptococcus)...
We designed the killed swine influenza A virus (SwIAV) H1N2 antigen (KAg) with polyriboinosinic:polyribocytidylic acid [(Poly(I:C)] adsorbed corn-derived Nano-11 particle based nanovaccine called Nano-11-KAg+Poly(I:C), and evaluated its immune correlates in maternally derived antibody (MDA)-positive pigs against a heterologous H1N1 SwIAV infection. Immunologically, tracheobronchial lymph nodes (TBLN) detected enhanced H1N2-specific cytotoxic T-lymphocytes (CTLs) Nano-11-KAg+Poly(I:C)...
This study focuses on the development and characterization of an intranasal vaccine platform using adjuvanted nanoparticulate delivery swine influenza A virus (SwIAV). The employed whole inactivated H1N2 SwIAV as antigen STING-agonist ADU-S100 adjuvant, with both surface adsorbed or encapsulated in mannose-chitosan nanoparticles (mChit-NPs). Optimization mChit-NPs included evaluating size, zeta potential, cytotoxicity, a 1:9 mass ratio to NP demonstrating high loading efficacy non-cytotoxic...
Human intravenous immunoglobulin ( IVI g) is constituted by pooled (IgG) from several thousand healthy donors. g consists of antibodies to external antigens and naturally occurring autoantibodies NA bs) with germline or close configurations. The bs are independent deliberate immunization cognate antigens. It well established that play an important role in maintaining tissue immune homeostasis. therefore logical attribute the therapeutic beneficial effects bs. Apart immunodeficiency...
The protozoan parasite Toxoplasma gondii is an important human and veterinary pathogen. Asexual replication of T. in humans intermediate hosts characterized by two forms: rapidly growing “tachyzoites” latent “bradyzoite” tissue cysts. Tachyzoites are responsible for acute illness congenital neurological birth defects, while the more slowly dividing bradyzoite form can remain within tissues many years, representing a threat to immunocompromised patients. We have developed genetic screen...
The development of cross-protective vaccines against the zoonotic swine influenza A virus (swIAV), a potential pandemic-causing agent, continues to be an urgent global health concern. Commercially available provide suboptimal cross-protection circulating subtypes swIAV, which can lead worldwide economic losses and poor zoonosis deterrence. limited efficacy current swIAV demands innovative strategies for next-generation vaccines. Considering that intramuscular injection is standard route...
The cytosolic protein degradation pathway, involving ATP-dependent proteases and ATP-independent peptidases, is important for modulating several cellular responses. involvement of pathogen-encoded well established during infection. However, the roles peptidases in this process are not studied. functional role Peptidase N (PepN), an enzyme belonging to M1 family, systemic infection mice by Salmonella enterica serovar Typhimurium (Salmonella typhimurium) was investigated. In a model infection,...
Current research efforts require a broad range of immune reagents, but those available for pigs are limited. The goal this study was to generate priority reagents and pipeline them marketing. Our were aimed at the expression soluble swine cytokines production panels monoclonal antibodies (mAbs) these proteins. Swine interleukin-17A (IL-17A) Interferon-gamma (IFNγ) recombinant proteins produced using yeast used antibody (mAb) resulting in mAbs. We screened each mAb cross-species reactivity...
Intravenous immunoglobulin (IVIG) is one of the widely used immunotherapeutic molecules in therapy autoimmune and inflammatory diseases. Previous reports demonstrate that anti-inflammatory actions IVIG implicates suppression macrophage activation release their mediators. However, macrophages are highly plastic depending on microenvironmental signals, can be polarized into pro-inflammatory classic (M1) or alternative (M2) type. This plasticity raised additional questions role towards...