A5044467468- Enzyme Production and Characterization
- Fungal Biology and Applications
- Environmental Toxicology and Ecotoxicology
- Pesticide Exposure and Toxicity
- Pesticide and Herbicide Environmental Studies
- Medicinal Plants and Neuroprotection
- Biochemical and Structural Characterization
- Advanced biosensing and bioanalysis techniques
- Sirtuins and Resveratrol in Medicine
- Medicinal Plants and Bioactive Compounds
- Microbial Metabolism and Applications
- Plant tissue culture and regeneration
- Biosensors and Analytical Detection
- Biological Control of Invasive Species
- Plant and animal studies
- Protease and Inhibitor Mechanisms
- Transgenic Plants and Applications
- Biochemical effects in animals
- Venomous Animal Envenomation and Studies
- Plant Parasitism and Resistance
- Botanical Research and Applications
- Cholinesterase and Neurodegenerative Diseases
- Bioactive natural compounds
- Aquatic Ecosystems and Biodiversity
- Hemostasis and retained surgical items
Tribhuvan University
2010-2023
University of Antwerp
2021
Virginia Commonwealth University
2018-2020
Chosun University
2005-2014
Gwangju University
2010
Kaempferol, a dietary flavonoid and phyto-oestrogen, is known to have anti-inflammatory properties. Microglial activation has been implicated in various neurodegenerative diseases. Anti-inflammatory effects of kaempferol the underlying mechanisms were investigated by using LPS-stimulated microglial BV2 cells.Cell viability was measured MTT neutral red assays. elisa, Western blot, immunocytochemistry electrophoretic mobility-shift assay used analyse NO, PGE(2) , TNF-α IL-1β production,...
This study describes the diversity and current status of Small Indigenous Fish Species (SIS) in rivers lakes Western Nepal. sampling was conducted between 2018 2022 across five major water bodies using cast nets traditional fishing gear, with assistance from local fishermen. A total 10,976 individual fish were recorded, representing 61 species 9 orders, 21 families, 43 genera, including six apart SIS category. The identified grouped into nine categories: Barbs Minnows, Catfishes, Eels,...
In this study we purified a fibrinolytic enzyme from the culture supernatant of Flammulina velutipes mycelia by ion exchange and gel filtration chromatographies, it was designated as F. protease (FVP-I). This purification protocol resulted in 18.52-fold at final yield 0.69%. The molecular mass estimated to be 37 kDa SDS–PAGE, fibrin-zymography size exclusion FPLC. effectively hydrolyzed fibrin, preferentially digesting α-chain over β-and γ–γ chain. Optimal activity found occur pH 6.0...
Lysozyme is a conserved antimicrobial enzyme and has been cited for its role in immune modulation. Increase lysozyme concentration body fluids also regarded as an early warning of some diseases such Alzheimer’s, sarcoidosis, Crohn’s disease, breast cancer. Therefore, method sensitive selective detection can benefit many different areas research. In this regard, several aptamers that are specific to have developed, but there still lack sensitive, specific, quantitative. work, we demonstrated...
Multiplex detection of biomolecules is important in bionanotechnology and clinical diagnostics. Multiplexing using engineered solutions such as microarrays, synthetic nanopores, DNA barcodes promising, but they require sophisticated design/engineering typically yield semiquantitative information. Single-molecule fluorescence resonance energy transfer (smFRET) an attractive tool this regard it enables both sensitive quantitative detection. However, multiplexing with smFRET remains a great...
The cytosine (C)-rich sequences that can fold into tetraplex structures known as i-motif are prevalent in genomic DNA. Recent studies of i-motif-forming have shown increasing evidence their roles gene regulation. However, most these been performed short single-stranded oligonucleotides, far from the intracellular environment. In cells, flanked by DNA duplexes and packed genome. Therefore, exploring conformational dynamics kinetics under such topologically constrained environments is highly...
In this study, we analyzed the antioxidant and antimelanogenic properties of a variety solvent extracts pre-bloom full-bloom chestnut flowers. Among extracts, methanol extract (preM) an ethanol (preE) showed highest amounts phenolics (467.92±0.45 456.24±5.88 mg gallic acid equivalent/g extract) flavonoids (60.96±1.86 41.59±8.57 quercetin extract). These exhibited DPPH radical reducing activities, as well greatest mushroom tyrosinase inhibition activity. addition, preE effectively protected...
Prism-based total internal reflection fluorescence (pTIRF) microscopy is one of the most widely used techniques for single molecule analysis a vast range samples including biomolecules, nanostructures, and cells, to name few. It allows excitation surface bound molecules/particles/quantum dots via evanescent field confined region space, which beneficial not only detection but also dynamics acquiring kinetics data. However, there neither commercial microscope available purchase nor detailed...
Abstract Objectives The present study investigated the neuroprotective effects of Rhus verniciflua Stokes (RVS) leaf extract on rotenone-induced apoptosis in human dopaminergic cells, SH-SY5Y. Methods Cells were pretreated with RVS for 1 h then treated vehicle or rotenone 24 h. Cell viability, cell cytotoxicity, morphology and nuclear examined by MTT assay, lactate dehydrogenase release phase contrast microscopy staining Hoechast 33342, respectively. Reactive oxygen species measured...