To investigate whether apoptosis occurs in the synovium of rheumatoid arthritis (RA), and intermediate molecules operating this process.DNA fragmentation was detected by situ nick end labelling (ISNEL) patients with RA (n = 11) control femoral neck fracture 5). The expression proteins p53, p21WAFI/CIPI, c-myc, proliferating cell nuclear antigen (PCNA), Bcl-2 also examined immunohistochemistry.ISNEL positive synovial cells specific morphology were extremely limited areas only two tissue...

Retinoic acids, a group of natural and synthetic vitamin A derivatives, have potent antiproliferative anti-inflammatory properties. Recently, retinoic acids were reported to inhibit Th1 cytokine production. We investigated the effects acid on lupus nephritis in model NZB/NZW F(1) (NZB/W F(1)) mice. Three-month-old NZB/W mice separated into two groups: one treated with all-trans-retinoic (ATRA; 0.5 mg i.p., three times weekly for 7 mo) saline as control. Compared controls, ATRA-treated...

Abstract Autoimmune disease in Fas-deficient MRL-Faslpr mice is dependent on infiltrating autoreactive leukocytes and autoantibodies, IFN-γ plays an important role the pathogenesis. As IL-18 capable of inducing production T cells, we hypothesized that signaling through IL-18R involved To investigate impact this autoimmune disease, generated strain deficient IL-18Rα. Compared with wild-type strain, IL-18Rα-deficient survived longer showed a significant reduction renal pathology, including...

Corticosteroids are highly effective anti-inflammatory or immunosuppressive drugs used commonly to treat human systemic lupus erythematosus (SLE). All-trans-retinoic acid (ATRA), which belongs a class of retinoids that exert immunomodulatory and functions, can also suppress the development nephritis in an animal model. However, both agents inflict serious adverse effects. Here, we have asked whether ATRA serve as steroid-sparing drug treatment nephritis. To examine efficacy combining...

We report the case of a patient who developed antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) after receiving coronavirus disease 2019 (COVID-19) vaccine BNT162b (Pfizer–BioNTech). A 37-year-old Japanese woman had been taking propylthiouracil for Graves’ disease. She erythema on her forearm 12th day first dose vaccine, fever 13th day, and redness swelling left auricle 25th day. Her serum myeloperoxidase-ANCA proteinase 3-ANCA levels, which were negative before...

<i>Background:</i> Retinoic acids, a group of natural and synthetic vitamin A derivatives, have potent anti-proliferative, anti-inflammatory anti-fibrotic properties. We investigated the therapeutic effect all-<i>trans</i>-retinoic acid (ATRA) on unilateral ureteral obstruction (UUO) model mice. <i>Methods:</i> First, to evaluate prophylactic effect, we administered 0.5 mg ATRA for 3 days before UUO (UUO ATRA). Then, effects, after (Day3 compared...

Osteoporosis is one of the serious adverse effects associated with glucocorticoid therapy. Although bisphosphonates have been used for glucocorticoid-induced osteoporosis (GIO), some patients shown an inadequate response. In such cases, denosumab or teriparatide are used. However, there no consensus on which these two drugs superior. We prospectively compared denosumab's and teriparatide's bone mineral density (BMD) in GIO prior bisphosphonate treatment. After receiving oral ≥2 years, low...

AbstractThe T‐helper 1/T‐helper 2 (Th1/Th2) cell balance was examined in 6‐month‐old New Zealand black/white F1 (B/WF1) mice treated with an immunosuppressive agent, FK506. The survival rate of 10 mg/kg/day FK506 7/8, while that those 2.5 5/8, and 4/8 after treatment for 8 weeks placebo. Proteinuria, which already positive all before the treatment, seven eight remained mildly (≤1+), six placebo showed severe proteinuria (≥2+). Pathological changes kidneys were less than or Expression mRNA...

Lupus cystitis is a rare manifestation in systemic lupus erythematosus (SLE); it usually occurs association with gastrointestinal manifestations. We report two cases of without bladder irritation symptoms. Both developed severe abdominal pain, nausea, and diarrhea showed no The diagnosis was made by ultrasonography biopsy. patients were treated intravenous methylprednisolone pulse therapy followed oral prednisolone. Their symptoms ameliorated, hydroureteronephrosis improved. Thus, when...

Renal interstitial fibrosis is a common lesion in the process of various progressive renal diseases. Interleukin (IL)-18 proinflammatory cytokine that plays an important role induction Th1 responses and associated with fibrosis, but mechanism remains unclear. Here we used IL-18 receptor alpha knockout (IL-18Rα KO) mice to investigate IL-18Rα signaling pathway murine model unilateral ureteral obstruction. Rα KO showed decreased increased infiltration CD4+ T cells Foxp3+ regulatory (Tregs)...

Objectives In our previous 24-month study, we observed that teriparatide had some advantages over denosumab for bone mineral density (BMD) in glucocorticoid-induced osteoporosis (GIO) patients with prior bisphosphonate treatment. We conducted this extension study to investigate whether the advantage of obtained first 2 years would be maintained after switch denosumab. Materials and Methods switched who completed daily treatment (switch group, n=18) compared their BMD every 6 months up 48...

Relapsing polychondritis (RP) is a rare autoimmune inflammatory disease characterized by recurrent inflammation and destruction of cartilage. Although auricular chondritis characteristic finding in RP, it can be difficult to diagnose the absence symptoms. A 64-year-old Japanese male was referred our hospital with fever respiratory distress. Contrast-enhanced computed tomography (CT) revealed bronchial wall thickening we suspected RP; however, he had no symptoms did not meet diagnostic McAdam...

It has been reported that apoptotic cells are increased in the peripheral blood from patients with systemic lupus erythematosus (SLE), where dysfunctions of T helper 1 (Th1) known. In order to study whether apoptosis Th1 is associated pathogenesis SLE, early various T-cell subsets were detected using fluorescence-labeled annexin V (AnV). AnV binding was most frequently observed CD4+CCR5+ cells, and rate (%) this subset higher SLE than normal controls (14.7 +/- 2.6), although active (43.6...

CD80 and CD86, expressed on the antigen-presenting cells (APCs) provide costimulatory signals for T lymphocytes. Recently, defective expression of has been reported in systemic lupus erythematosus (SLE) although its mechanism is unclear. Here, B7 antigens induced by interferon-g, interleukin-4 or granulocyte-macrophage stimulating-factor (GM-CSF) along differentiation process APCs was investigated. In contrast to CD14‡ GM-CSF reduced SLE. receptor (GMCSFR) down-regulated phorbol 12-myristate...