Sperm motility and hence male fertility strictly depends on proper development of the sperm tail its tight anchorage to head. The main protein outer dense fibers, ODF1/HSPB10, belongs family small heat shock proteins that function as molecular chaperones. However, impact ODF1 formation fecundity is unknown. We therefore generated mutant mice in which Odf1 gene was disrupted. Heterozygous are fertile while reduced, but Odf1-deficient infertile due detachment Although headless tails somehow...

The embryonic mouse fibroblast cell line NIH3T3 is widely used in life science research, including the study of cycle control and primary cilia. Fibroblasts are most important type connective tissue, as they produce components extracellular matrix determine tissue architecture. However, very heterogeneous consist subtypes specific to their organ residence, among others. was derived from whole embryos that developed pre-birth therefore likely composed different subtypes. Furthermore,...

CHARGE syndrome is an autosomal dominant disorder caused in about two-third of cases by mutations the CHD7 gene. For other genetic diseases e.g. hereditary spastic paraplegia, it was shown that interacting partners are involved underlying cause disease. These data encouraged us to search for binding a yeast two-hybrid library screen and CHD8 identified as partner. The result confirmed direct analysis, co-immunoprecipitation studies bimolecular fluorescence complementation assay. To...

A main structure of the mammalian sperm tail is a known as outer dense fibers whose molecular composition well their function are still mostly unknown. We report here isolation and characterization new cDNAs (<i>odf2</i>) that identifies highly variable gene locus encoding fiber proteins. Transcription <i>odf2</i> restricted to testis more specifically round spermatids. starts in step 6 spermatids, which coincides with transcription major protein <i>odf1</i> (Burmester, S., Hoyer-Fender, S....

SUN domain proteins are integral of the inner nuclear membrane and functions in linkage lamina to cytoskeleton. Moreover, seem mediate tethering centrosome membrane, they involved telomere attachment envelope meiotic cells, germ cell development invertebrates. In contrast widely expressed mammals, SUN1 SUN2, which have been analysed great detail, there is virtually nothing known about testicular proteins. Since a hallmark male profound remodelling envelope, emphasized, for example, by...

Abstract Regular use of marijuana during adolescence enhances the risk long‐lasting neurobiological changes in adulthood. The present study was aimed at assessing effect long‐term administration synthetic cannabinoid WIN55212.2 young adult mice. Adolescent mice aged 5 weeks were subjected daily to pharmacological action for 3 and then left undisturbed their home cage a 5‐week period finally evaluated by behavioral testing. Mice that received drug showed memory impairment Morris water maze,...

Terminal differentiation of male germ cells into functional spermatozoa requires shaping and condensation the nucleus as well formation sperm specific structures. A transient microtubular structure, manchette, is mandatory for head development connecting piece tail. The or head-to-tail coupling apparatus (HTCA) mediates tight linkage tail causing decapitation infertility when faulty. Using mice experimental model, several proteins have already been identified affecting either complex...

Outer dense fibre protein 2 (ODF2) is a major of sperm tail outer fibres which are prominent tail-specific cytoskeletal structures. Moreover, ODF2 was also identified as widespread component the centrosomal scaffold and found to associate preferentially with appendages mother centriole [Nakagawa, Y., Yamane, Okanoue, T., Tsukita, S. (2001) Mol. Biol. Cell 12, 1687-1697]. Secondary structure predictions indicated an overall coiled-coil putative forming capacity. To investigate its potential...

The small heat shock protein ODF1/HSPB10 is essential for male fertility in mice. Targeted deletion of Odf1 resulted acephalic sperm homozygous mice mixed background (C57BL/6J//129/Sv), whereas heterozygous animals are fully fertile. To further elucidate the function ODF1, we generated incipient congenic with targeted by successive backcrossing on 129/Sv background. We observed that fecundity Odf1(+/-) was severely reduced over backcross generations. However, neither aberrant parameters nor...

Complementary DNA clones for the boar preproacrosin have been isolated from a randomly primed testis cDNA library in λgt10 and an oligo(dT)‐primed λt11. The nucleotide sequence of 1418‐bp insert includes 46‐bp 5′‐untranslated region, open reading frame 1248 bp corresponding to 416 amino acids (45.59 kDa) 121‐bp 3′‐untranslated region. deduced acid active‐site residues histidine, asparagine serine catalytic triad proteinase super‐family is colinear with that determined by sequencing acrosin...

The outer dense fibers (ODF) of the mammalian sperm tail comprise a unique, specialized, and very prominent structure, consisting nine surrounding axoneme. ODF may play an important but as yet undefined role in morphology, integrity function. Study is hampered by insufficient knowledge their protein composition genetic regulation synthesis. We report here on characterization cDNAs encoding Odf2 proteins mouse sperm. isolated two cDNA clones with variable 5′ regions. Variability sequence...

Creb3l4 belongs to the CREB/ATF family of transcription factors that are involved in mediating response intracellular signaling. This study shows is expressed at low levels all organs and different stages embryogenesis but present very high testis, particularly postmeiotic male germ cells. In contrast CREB3L4 human prostate, which specific expression was detected, transcripts mouse prostate could be detected only by RT-PCR. To identify physiological function Creb3l4, murine gene inactivated...

The formation of the sex vesicle, or XY body, during male meiosis and pairing chromosomes are thought to be essential for successful spermatogenesis. Despite its cytological discovery a century ago, mechanism body formation, particularly heterochromatinization chromosomes, has remained unclear. HP1 class chromobox genes encode proteins involved in packaging chromosomal DNA into repressive heterochromatin domains, as seen, example, position-effect variegation. Study distribution murine...

Mutations in either the Drosophila melanogaster pelota or pelo gene Saccharomyces cerevisiae homologous gene, DOM34, cause defects of spermatogenesis and oogenesis Drosophila, delay growth failure sporulation yeast. These phenotypes suggest that is required for normal progression mitotic meiotic cell cycle. To determine role mouse development cycle, we have established a targeted disruption PELO: Heterozygous animals are variable fertile. Genotyping progeny heterozygous intercrosses shows...

Abstract Outer dense fibers (ODFs) are located on the outside of axoneme in midpiece and principal piece mammalian sperm tail may help to maintain passive elastic structures recoil tail. We have identified describe here a human gene that is homologous Mst(3)CGP family Drosophila melanogaster encodes an ODF protein 241 amino acids. The transcribed region has size ˜lkb contains two exons 416 bp 406 bp, respectively, not including 3′ untranslated region. expressed testis but spleen, kidney, or...

Abstract Background Pelota (PELO) is an evolutionary conserved protein, which has been reported to be involved in the regulation of cell proliferation and stem self-renewal. Recent studies revealed essential role PELO No-Go mRNA decay, by with translational stall are endonucleotically cleaved degraded. Further, PELO-deficient mice die early during gastrulation due defects and/or differentiation. Results We show here that associated actin microfilaments mammalian cells. Overexpression human...

Abstract Development of spermatozoa requires remodelling and formation particular structures. In elongating spermatids, the transient microtubular manchette contributes to head–tail coupling apparatus (HTCA) sperm tail. The HTCA derives from centrosome in that proximal centriole inserts into nuclear indentation distal gives rise flagellum. Although impairments tail cause male infertility their molecular constituents are only partially known. WD40-protein CFAP52 is implicated motile cilia,...