A5017044301- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Studies on Chitinases and Chitosanases
- Enzyme Production and Characterization
- Ginseng Biological Effects and Applications
- CRISPR and Genetic Engineering
- Legume Nitrogen Fixing Symbiosis
- GABA and Rice Research
- Medicinal Plants and Neuroprotection
- Plant-Microbe Interactions and Immunity
- Peanut Plant Research Studies
- Bacteriophages and microbial interactions
- Chemical and Physical Properties in Aqueous Solutions
- Toxin Mechanisms and Immunotoxins
- Coconut Research and Applications
- Biofuel production and bioconversion
- Protein Hydrolysis and Bioactive Peptides
- Microbial bioremediation and biosurfactants
- Pharmacological Effects of Natural Compounds
- Plant biochemistry and biosynthesis
- Genetically Modified Organisms Research
- Microbial Metabolism and Applications
- Escherichia coli research studies
- Phytochemical compounds biological activities
- Microbial Metabolic Engineering and Bioproduction
Hue University
2015-2024
Baria Vungtau University
2022
Czech Academy of Sciences, Institute of Biotechnology
2015
Jeonbuk National University
2003-2010
Korea Basic Science Institute
2010
University of Utah
2009
Vietnam National University Ho Chi Minh City
2004
Trichoderma species were known as biological control agents against phytopathogenic fungi because they produce a variety of chitinases. Chitinases are hydrolytic enzymes that break down glycosidic bonds in chitin, major component the cell walls fungi. The present study shows extracellular chitinase activity reached maximum value approximately 22 U/mL after 96 h T. asperellum PQ34 strain culture. optimal temperature and pH enzyme 40°C 7, respectively, whereas thermal stability range from 25°C...
Four Trichoderma strains (CH2, SH16, PQ34, and TN42) were isolated from soil samples collected Quang Tri Thua Thien Hue provinces in Vietnam. The exhibited high chitinolytic secretion. Strain PQ34 formed the largest zone of chitinase-mediated clearance (> 4 cm diameter) agar containing 1% (w/v) colloidal chitin. Analysis internal transcribed spacer regions these indicated that they asperellum. molecular weights chitinases approximately 42 kDa. Chitinase genes (chi42) T. asperellum TN42, CH2,...
In this study, we report the expression level of CaSQS, CabAS and CaCYS, genes involved in phytosterol triterpene metabolic pathway centella (Centella asiatica (L.) Urban), cells elicited with salicylic acid (50-200 µM). Reverse transcription-polymerase chain reaction (RT-PCR) Northern blot analysis indicated CabAS, CaCYS expressed both wild-type cultured (with without elicitation). cells, expressions showed strong dependence on concentration elicitation day. The highest gene was found 100...
Geobacillus sp. JF8 is a thermophilic biphenyl and naphthalene degrader. To identify the degradation genes, cis-naphthalene dihydrodiol dehydrogenase was purified from naphthalene-grown cells, its N-terminal amino acid sequence determined. Using DNA probe encoding region of dehydrogenase, 10-kb fragment isolated. Upstream nahB, gene for there were two open reading frames which designated as nahAc nahAd, respectively. The products nahAd predicted to be alpha beta subunit ring-hydroxylating...
Eurycomanone is found in the Eurycoma longifolia Jack (Simaroubaceae) tree, exhibits significant antimalarial activity, improves spermatogenesis, suppresses expression of lung cancer cell tumour markers and regulates signalling pathways involved proliferation, death inflammation.Establishment suspension culture E. to determine eurycomanone accumulation during cultures.Callus was cultured MS medium supplemented with 0.8% agar, 30/L sucrose, 1.25 mg/L NAA 1 KIN for biomass production. Cell...
To investigate the effects of some culture conditions on production asiaticoside from centella (Centella asiatica L. Urban) cells cultured in 5-L bioreactor. The centell cell suspension was conducted bioreactor to growth and accumulation under various conditions. Asiaticoside content determined by HPLC analysis. results showed that peaked after 24 d at an agitation speed 150 r/min aeration rate 2.5 L/min. biomass reached a maximum value 302.45 g fresh weight (31.45 dry weight) index 3.03...
Abstract Chitinases are enzymes that catalyze the degradation of chitin, a major component cell walls pathogenic fungi and cuticles insects, gaining increasing attention for control fungal pathogens insect pests. Production recombinant chitinase in suitable host can result more pure product with less processing time significantly larger yield than produced by native microorganisms. The present study aimed to express synthetic chi42 gene (syncodChi42), which was optimized from Trichoderma...
Truong Thi Phuong Lan · Nguyen Duc Huy Ngoc Luong Hoang Tan Quang Trinh Huu Le Anh Thu Xuan Loc. J Plant Biotechnol 2019;46:172-9. https://doi.org/10.5010/JPB.2019.46.3.172
The present study is focused on the production and purification of nattokinase, a fibrinolytic protease, from B. subtilis TH9 (NatTH9) based an aqueous two-phase system (ATPS) technique. results showed that optimal ATPS for NatTH9 recovery was 20% (w/v) polyethylene glycol 6000 15% potassium phosphate at pH 8. partitioning coefficient, yield, activity were 6.25, 76.7%, 547.02 U/mg, respectively. purified demonstrated ability to degrade fibrin dissolve clot. Fibrin zymography three clear...
Accumulation of madecassoside -a major component centelloside -in centella (Centella asiatica (L