A5052272190- Fatty Acid Research and Health
- Cholesterol and Lipid Metabolism
- COVID-19 Clinical Research Studies
- Adipose Tissue and Metabolism
- Infection Control and Ventilation
- Long-Term Effects of COVID-19
- Microbial bioremediation and biosurfactants
- Metal complexes synthesis and properties
- Peroxisome Proliferator-Activated Receptors
- Virus-based gene therapy research
- Respiratory Support and Mechanisms
- Animal Virus Infections Studies
- Hormonal and reproductive studies
- Viral gastroenteritis research and epidemiology
- Liver physiology and pathology
- Neurogenesis and neuroplasticity mechanisms
- Glaucoma and retinal disorders
- SARS-CoV-2 detection and testing
- Diet, Metabolism, and Disease
- Biopolymer Synthesis and Applications
- Advanced Chemical Sensor Technologies
- Epigenetics and DNA Methylation
- Metabolomics and Mass Spectrometry Studies
- Dental Research and COVID-19
- Corneal surgery and disorders
Kinjo Gakuin University
2013-2023
Aizawa Hospital
2020-2022
Takara (Japan)
2020
Okayama University
2019
University of British Columbia
2006-2012
Osaka University
2006-2007
Tokyo Institute of Technology
2004-2005
Nagoya City University
2003
Kobe City Medical Center General Hospital
1997-2001
Rhodococcus sp. RHA1 (RHA1) is a potent polychlorinated biphenyl-degrading soil actinomycete that catabolizes wide range of compounds and represents genus considerable industrial interest. has one the largest bacterial genomes sequenced to date, comprising 9,702,737 bp (67% G+C) arranged in linear chromosome three plasmids. A targeted insertion methodology was developed determine telomeric sequences. RHA1's 9,145 predicted protein-encoding genes are exceptionally rich oxygenases (203)...
Many therapeutic interventions using neurotrophic factors or pharmacological agents have focused on secondary degeneration after spinal cord injury (SCI) to reduce damaged areas and promote axonal regeneration functional recovery. Hepatocyte growth factor (HGF), which was identified as a potent mitogen for mature hepatocytes mediator of inflammatory responses tissue injury, has recently been highlighted angiogenic in the central nervous system (CNS). In present study, we revealed that extent...
Occlusive cerebrovascular disease leads to brain ischemia that causes neurological deficits. Here we introduce a new strategy combining mesenchymal stromal cells (MSCs) and ex vivo hepatocyte growth factor (HGF) gene transferring with multimutated herpes simplex virus type-1 vector in rat transient middle cerebral artery occlusion (MCAO) model. Gene-transferred MSCs were intracerebrally transplanted into the rats' ischemic brains at 2h (superacute) or 24 h (acute) after MCAO. Behavioral...
Patients with nonalcoholic fatty liver disease are increasing worldwide, and preventive measures an urgent need primary concern today.This study aimed to develop clarify the usefulness of SHRSP5/Dmcr rat, derived from a stroke-prone spontaneously hypertensive as novel animal model for time-course analysis steatohepatitis severe fibrosis progression often observed in disease.Ten-week-old male rats were divided into six groups: half fed high-fat high-cholesterol-containing diet (HFC diet),...
ABSTRACT Rhodococcus sp. RHA1 grows on a broad range of aromatic compounds and vigorously degrades polychlorinated biphenyls (PCBs). Previous work identified genes encoding multiple isozymes for most the seven steps biphenyl (BPH) pathway, provided evidence coexpression some these isozymes, indicated involvement enzymes in degradation BPH, ethylbenzene (ETB), PCBs. To investigate expression better understand how they contribute to robust degradative capacity RHA1, we comprehensively analyzed...
Rhodococcus jostii RHA1, a catabolically diverse soil actinomycete, is highly resistant to long-term nutrient starvation. After 2 years of carbon starvation, 10% the bacterial culture remained viable. To study molecular basis such resistance, we monitored abundance about 1,600 cytosolic proteins during 2-week period source (benzoate) Hierarchical cluster analysis elucidated 17 major protein clusters and showed that most changes occurred transition stationary phase. We identified 196...
Pirarubicin (THP) has shown equal or superior cytotoxicity compared to doxorubicin. One of the main anticancer actions doxorubicin is believed be involved in ROS (reactive oxygen species) generation. Therefore, mechanisms THP may involve The aim this study was clarify THP-induced apoptosis through generation.We analyzed apoptotic events induced by HL-60 cells and HP100 cells, hydrogen peroxide (H2O2)-resistant derived from HL-60.The apparent could detected at above 0.1 μM after 24-h...
Obesity, hypertension, diabetes, and specific ethnicities (Black Hispanic) have been reported to be common comorbidities possible risk factors for the severity of both coronavirus disease 2019 (COVID-19) H1N1 influenza infections.1, 2 Thus, it is important understand why these four are COVID-19 infections, whether a mechanism exists. Respiratory failure most pathology that contributes infections. Patients with obesity show restrictive breathing pattern reduced lung volumes. In severe cases,...
Bacillus sp. JF8 is a thermophilic polychlorinated biphenyl (PCB) degrader, which utilizes and naphthalene. A thermostable, Mn-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase, BphC_JF8, has been characterized previously. Upstream of bphC are five ORFs exhibiting low homology with, different gene order from, previously bph genes. From the 5' to 3' direction genes are: putative regulatory (bphR), hydrolase (bphD), large small subunits ring-hydroxylating dioxygenase (bphA1A2), cis-diol...
Geobacillus sp. JF8 is a thermophilic biphenyl and naphthalene degrader. To identify the degradation genes, cis-naphthalene dihydrodiol dehydrogenase was purified from naphthalene-grown cells, its N-terminal amino acid sequence determined. Using DNA probe encoding region of dehydrogenase, 10-kb fragment isolated. Upstream nahB, gene for there were two open reading frames which designated as nahAc nahAd, respectively. The products nahAd predicted to be alpha beta subunit ring-hydroxylating...
Epidemiologic and ecologic studies suggest that dietary fat plays an important role in the development of obesity. Certain Wistar rat strains do not become obese when fed high-fat diets unlike others. In a preliminary study, we confirmed Slc:Wistar/ST rats did diets. The mechanisms governing response hepatic lipid-metabolizing enzymes to large quantities lipids consumed by obesity-resistant animals are unknown. aim present study is examine how metabolize various types why they obese. For...
The aim of this study was to investigate the effects administration oral arachidonic acid (AA) in rats with or without dextran sulphate sodium (DSS)-induced inflammatory bowel disease. Male Wistar were administered AA at 0, 5, 35 240 mg/kg daily by gavage for 8 weeks. Inflammatory disease induced replacing drinking water 3 % DSS solution during last 7 d dosing period. These animals passed loose stools, diarrhoea and red-stained faeces. Cyclo-oxygenase-2 concentration myeloperoxidase activity...
Abstract Identifying biomedical and socioeconomic predictors of the number deaths caused by COVID-19 can help development effective interventions. In this study, we used hypothesis-driven regression approach to test hypothesis that mask wearing rate, along with age obesity, largely predict cumulative across countries. Our models explained 69% variation in per million (March June 2020) among 22 countries, identifying face rate March as an important predictor. The predicted our elastic net...
Background/Aim: One mechanism of the anticancer action anthracyclines is believed to be oxidative DNA damage. Previously, we reported that doxorubicin induced damage in presence Cu(II). However, pirarubicin-induced has not been well clarified. Materials and Methods: by pirarubicin Cu(II) was analyzed using pBR322 plasmid DNA. O<sub>2</sub><sup>•−</sup> derived from detected cytochrome c reduction. Results: Pirarubicin Scavenger experiments suggest reactive species are generated...
This study aimed to investigate aclarubicin (ACR)-induced oxidative DNA damage and apoptosis.ACR-induced apoptosis was analyzed using HL-60 leukemia cells HP100 cells, hydrogen peroxide (H2O2)-resistant derived from cells. ACR-induced plasmid DNA.HL-60 were more sensitive ACR than In ladder formation caspase-3/7 activity induced by suppressed or delayed in comparison those occurred the presence of Cu(II), scavenger experiments showed that reactive species causing appeared be generated H2O2...
A 10 kb DNA fragment was isolated using a probe derived from the N-terminal amino acid sequence of extradiol dioxygenase purified naphthalene-grown Bacillus sp. JF8, thermophilic naphthalene and polychlorinated biphenyl degrader. The cloned had six open reading frames, designated nahHLOMmocBnahC based on homology, which products NahH_JF8 NahC_JF8 were dioxygenases. Although exhibit low homology to known dioxygenases, active-site residues metal ion ligands are conserved. presence Mn(II) in...
Background/Aim: The aim of the present study was to investigate whether idarubicin (IDR) induces oxidative DNA damage in presence copper (II). Materials and Methods: evaluated by pBR322 plasmid cleavage. formation stress markers [O<sub>2</sub><sup>•−</sup> 8-hydroxy-2'-deoxyguanosine (8-OHdG)] analysed. Results: IDR induced O<sub>2</sub><sup>•−</sup> 8-OHdG generation Conclusion: Since it has been reported that concentration serum cancer patients is higher than healthy groups, IDR-induced...