A5011762151- Microbial bioremediation and biosurfactants
- Microbial Metabolic Engineering and Bioproduction
- Enzyme-mediated dye degradation
- Metal-Catalyzed Oxygenation Mechanisms
- Wastewater Treatment and Nitrogen Removal
- Biofuel production and bioconversion
- Biochemical and biochemical processes
- Microbial metabolism and enzyme function
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Toxic Organic Pollutants Impact
- Amino Acid Enzymes and Metabolism
- Chromium effects and bioremediation
- Genomics and Phylogenetic Studies
- Lignin and Wood Chemistry
- Pharmacogenetics and Drug Metabolism
- Microbial Community Ecology and Physiology
- Enzyme Catalysis and Immobilization
- Microbial Natural Products and Biosynthesis
- Membrane Separation Technologies
- Plant biochemistry and biosynthesis
- Pesticide and Herbicide Environmental Studies
- Chemical Reactions and Isotopes
- Analytical Chemistry and Chromatography
- Microbial Fuel Cells and Bioremediation
Nagaoka University of Technology
2014-2024
Chubu University
2017-2021
Nagaoka University
1997-2017
National Institute for Environmental Studies
2017
Keio University
2017
Panasonic (Japan)
2013
Unitika (Japan)
2013
Forestry and Forest Products Research Institute
2009
Tokyo University of Agriculture and Technology
2009
SPring-8
2002-2008
Rhodococcus sp. RHA1 (RHA1) is a potent polychlorinated biphenyl-degrading soil actinomycete that catabolizes wide range of compounds and represents genus considerable industrial interest. has one the largest bacterial genomes sequenced to date, comprising 9,702,737 bp (67% G+C) arranged in linear chromosome three plasmids. A targeted insertion methodology was developed determine telomeric sequences. RHA1's 9,145 predicted protein-encoding genes are exceptionally rich oxygenases (203)...
We have characterized a biphenyl degrader, Rhodococcus sp. strain RHA1. Biphenyl-grown cells of RHA1 efficiently transformed 45 components in the 62 major peaks polychlorinated (PCB) mixture Kanechlors 200, 300, 400, and 500 within 3 days, which includes mono- to octachlorobiphenyls. Among intermediate metabolites PCB transformation, di- trichlorobenzoic acids were identified. The gradual decrease these chlorobenzoic during incubation indicated that would also be degraded by this strain....
Two genes involved in the degradation of biphenyl were isolated from a gene library polychlorinated biphenyl-degrading soil bacterium, Pseudomonas sp. strain KKS102, by using broad-host-range cosmid vector, pKS13. When 3.2-kilobase (kb) PstI fragment 29-kb DNA insert was subcloned into pUC18 at site downstream lacZ promoter, Escherichia coli cells carrying this recombinant plasmid expressed 2,3-dihydroxybiphenyl dioxygenase activity. Nucleotide sequencing 3.2-kb revealed that there two open...
Rhodococcus sp. strain RHA1 is a gram-positive polychlorinated biphenyl (PCB) degrader which can degrade 10 ppm of PCB48 (equivalent to Aroclor1248), including tri-, tetra-, and pentachlorobiphenyls, in few days. We isolated the 7.6-kb EcoRI-BamHI fragment carrying catabolic genes determined their nucleotide sequence. On basis deduced amino acid sequence homology, we identified six bph genes, bphA1A2A3A4, bphB, bphC, that are responsible for initial three steps degradation. The order...
Pseudomonas paucimobilis UT26 is capable of growing on gamma-hexachlorocyclohexane (gamma-HCH). A genomic library P. was constructed in putida by using the broad-host-range cosmid vector pKS13. After 2,300 clones were screened gas chromatography, 3 showing gamma-HCH degradation detected. 5-kb fragment from one subcloned into pUC118, and subsequent deletion chromatography-mass spectrometry analyses revealed that a ca. 500 bp responsible for conversion to 1,2,4-trichlorobenzene via...
ABSTRACT Cleavage of the β-aryl ether linkage is most important process in lignin degradation. Here we characterize three tandemly located glutathione S -transferase (GST) genes, ligF , ligE and ligG from low-molecular-weight lignin-degrading Sphingomonas paucimobilis SYK-6, describe actual roles these genes cleavage. Based on identification reaction product by electrospray ionization-mass spectrometry, a model compound ether, α-(2-methoxyphenoxy)-β-hydroxypropiovanillone (MPHPV), was...
Vanillate and syringate are converted into protocatechuate (PCA) 3-O-methylgallate (3MGA), respectively, by O-demethylases in Sphingomonas paucimobilis SYK-6. PCA is further degraded via the 4,5-cleavage pathway, while 3MGA through multiple pathways which 4,5-dioxygenase (LigAB), 3,4-dioxygenase (DesZ), an unidentified O-demethylase gallate dioxygenase participants. For this study, we isolated a 4.7-kb SmaI fragment that conferred on Escherichia coli activity required for conversion of...
ABSTRACT The tfd genes of Ralstonia eutropha JMP134 are the only well-characterized set responsible for 2,4-dichlorophenoxyacetic acid (2,4-D) degradation among 2,4-D-degrading bacteria. A new family 2,4-D genes, cadRABKC , was cloned and characterized from Bradyrhizobium sp. strain HW13, a that isolated buried Hawaiian soil has never experienced anthropogenic chemicals. cadR gene inferred to encode an AraC/XylS type transcriptional regulator its deduced amino sequence. cadABC were predicted...
ABSTRACT We isolated Comamonas sp. strain E6, which utilizes terephthalate (TPA) as the sole carbon and energy source via protocatechuate (PCA) 4,5-cleavage pathway. Two almost identical TPA degradation gene clusters, tphR I C A2 A3 B A1 II , were from this strain. Based on amino acid sequence similarity, genes tphC tphA2 tphA3 tphB tphA1 predicted to code, respectively, for an IclR-type transcriptional regulator, a periplasmic binding receptor, large subunit of oxygenase component...
We isolated Comamonas sp. strain E6, which utilizes terephthalate (TPA) as the sole carbon and energy source via protocatechuate (PCA) 4,5-cleavage pathway. Two almost identical TPA degradation gene clusters, tphRICIA2IA3IBIA1I tphRIICIIA2IIA3IIBIIA1II, were from this strain. Based on amino acid sequence similarity, genes tphR, tphC, tphA2, tphA3, tphB, tphA1 predicted to code, respectively, for an IclR-type transcriptional regulator, a periplasmic binding receptor, large subunit of...
ABSTRACT Degradation of arylglycerol-β-aryl ether is the most important process in bacterial lignin catabolism. Sphingobium sp. strain SYK-6 degrades guaiacylglycerol-β-guaiacyl (GGE) to α-(2-methoxyphenoxy)-β-hydroxypropiovanillone (MPHPV), and then linkage MPHPV cleaved generate α-glutathionyl-β-hydroxypropiovanillone (GS-HPV) guaiacol. We have characterized three enantioselective glutathione S -transferase genes, including two genes that are involved cleavage enantiomers one gene...
Paenibacillus sp. (formerly Bacillus macerans) strain JJ-1b is able to grow on 4-hydroxybenzoate (4HB) as a sole source of carbon and energy known degrade 4HB via the protocatechuate (PCA) 2,3-cleavage pathway. However, none genes involved in this pathway have been identified. In study, we identified characterized for catabolic PCA pathway, which consisted praR praABEGFDCHI. Based enzyme activities cell extracts Escherichia coli carrying praI, praA, praH, praB, praC, praD, these were found...
ABSTRACT It has been suggested that a novel type of aromatic acid transporter, which is similar to the tripartite tricarboxylate transporter (TTT), involved in terephthalate (TPA) uptake by Comamonas sp. strain E6. This suggestion was based on presence putative TPA-binding protein gene, tphC , TPA catabolic operon. The gene essential for growth and genes encoding TTT-like substrate-binding proteins. Here we identified two sets E6 genes, tctBA tpiBA encode cytoplasmic transmembrane Disruption...
ABSTRACT Sphingomonas paucimobilis SYK-6 degrades syringate to 3- O -methylgallate (3MGA), which is finally converted pyruvate and oxaloacetate via multiple pathways in protocatechuate 4,5-dioxygenase, 3MGA dioxygenase, gallate dioxygenase are involved. Here we isolated the -demethylase gene ( desA ), complemented growth deficiency on of a Tn 5 mutant derivative strain. The located 929 bp downstream ferA , encoding feruloyl-coenzyme A synthetase, consists 1,386-bp open reading frame...
ABSTRACT Sphingomonas paucimobilis SYK-6 is able to grow on a wide variety of dimeric lignin compounds with guaiacyl moieties, which are converted into protocatechuate by the actions degradation enzymes in this strain. Protocatechuate key metabolite and it thought that degrades pyruvate oxaloacetate via 4,5-cleavage pathway. In 10.5-kb Eco RI fragment carrying 4,5-dioxygenase gene ( ligAB ) (Y. Noda, S. Nishikawa, K. Shiozuka, H. Kadokura, Nakajima, Yoda, Y. Katayama, N. Morohoshi, T....
A genomic library of Acetobacter aceti DNA was constructed by using a broad-host-range cosmid vector. Complementation spontaneous alcohol dehydrogenase-deficient mutant resulted in the isolation plasmid designated pAA701. Subcloning and deletion analysis pAA701 limited region that complemented deficiency dehydrogenase activity mutant. The nucleotide sequence this determined showed contained full structural gene for 72-kilodalton subunit enzyme complex. predicted amino acid homology with...
ABSTRACT Sphingomonas paucimobilis SYK-6 degrades ferulic acid to vanillin, and it is further metabolized through the protocatechuate 4,5-cleavage pathway. We obtained a Tn 5 mutant of SYK-6, FA2, which was able grow on vanillic but not acid. A cosmid complemented growth deficiency FA2 isolated. The 5.2-kb Bam HI- Eco RI fragment in this conferred transformation activity vanillin Escherichia coli host cells. sequencing analysis revealed genes ferB ferA fragment; these consist 852- 2,127-bp...
In Pseudomonas paucimobilis UT26, gamma-hexachlorocyclohexane (gamma-HCH) is converted to 2,5-dichloro-2,5-cyclohexadiene-1,4-diol (2,5-DDOL), which then metabolized 2,5-dichlorohydroquinone. Here, we isolated from the genomic library of UT26 two genes expressed 2,5-DDOL dehydrogenase activity when they were transformed into P. putida and Escherichia coli. Both gene products had an apparent molecular size 28 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The first gene,...
Proteomics and targeted gene disruption were used to investigate the catabolism of benzene, styrene, biphenyl, ethylbenzene in Rhodococcus jostii RHA1, a well-studied soil bacterium whose potent polychlorinated biphenyl (PCB)-transforming properties are partly due presence related Bph Etb pathways. Of 151 identified proteins, 22 Bph/Etb proteins among most abundant biphenyl-, ethylbenzene-, benzene-, styrene-grown cells. Cells grown on ethylbenzene, or benzene contained both enzymes at least...