A5035936638- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Enzyme function and inhibition
- Phenothiazines and Benzothiazines Synthesis and Activities
- Cholinesterase and Neurodegenerative Diseases
- Microbial bioremediation and biosurfactants
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Microbial Metabolic Engineering and Bioproduction
- Organometallic Compounds Synthesis and Characterization
- Metal complexes synthesis and properties
- Chromium effects and bioremediation
- Cystic Fibrosis Research Advances
- Trace Elements in Health
- Advanced Chemical Sensor Technologies
- Crystallography and molecular interactions
- Biofuel production and bioconversion
- bioluminescence and chemiluminescence research
- Microbial Community Ecology and Physiology
- Vibrio bacteria research studies
- Slime Mold and Myxomycetes Research
- Oral microbiology and periodontitis research
- Calcium Carbonate Crystallization and Inhibition
- Metalloenzymes and iron-sulfur proteins
Oklahoma State University
2014-2024
University of British Columbia
2003-2012
Oklahoma State University Oklahoma City
2011
ABSTRACT Genomic and proteomic approaches were used to investigate phthalate benzoate catabolism in Rhodococcus sp. strain RHA1, a polychlorinated biphenyl-degrading actinomycete. Sequence analyses identified genes involved the of ( ben ) pad ), uptake pat two branches β-ketoadipate pathway catRABC pcaJIHGBLFR ). The regulatory structural are separated by encoding cytochrome P450. contained on catabolic island that is duplicated plasmids pRHL1 pRHL2 includes predicted terephthalate tpa...
Aims: The aim of this work was to study the biodegradation benzyldimethylalkylammonium chloride (BAC) by Aeromonas hydrophila sp. K, an organism isolated from polluted soil and capable utilizing BAC as sole source carbon energy. Methods Results: High performance liquid chromatography gas chromatography–mass spectrometry (GC‐MS) analysis used degradation pathway. It shown that during biodegradation, formation benzyldimethylamine, benzylmethylamine, benzylamine, benzaldehyde benzoic acid...
In gram-negative bacteria, a pathway for aerobic degradation of phenylacetic acid (PAA) that proceeds via phenylacetyl-coenzyme A (CoA) and hydrolytic ring fission plays central role in the range aromatic compounds. contrast, PAA its are not well characterized gram-positive bacteria. cluster including 13 paa genes encoding enzymes orthologous to those bacteria was identified on chromosome Rhodococcus sp. strain RHA1. These were transcribed during growth PAA, with 11 apparently an operon...
Pseudomonas aeruginosa is an opportunistic pathogen that forms biofilms on mucous plugs in the lungs of cystic fibrosis (CF) patients, resulting chronic infections. Pulmonary P. isolates often display a mucoid (alginate-producing) phenotype, whereas non-mucoid strains are generally associated with acute We characterized cytosolic proteomes biofilm-associated and planktonic CF pulmonary isolate, FRD1, strain, PAO1. Since Ca2+ metabolism altered fluids, we also analysed effect proteome...
Proteomics and targeted gene disruption were used to investigate the catabolism of benzene, styrene, biphenyl, ethylbenzene in Rhodococcus jostii RHA1, a well-studied soil bacterium whose potent polychlorinated biphenyl (PCB)-transforming properties are partly due presence related Bph Etb pathways. Of 151 identified proteins, 22 Bph/Etb proteins among most abundant biphenyl-, ethylbenzene-, benzene-, styrene-grown cells. Cells grown on ethylbenzene, or benzene contained both enzymes at least...
ABSTRACT Pseudomonas aeruginosa is an opportunistic human pathogen that causes severe, life-threatening infections in patients with cystic fibrosis (CF), endocarditis, wounds, or artificial implants. During CF pulmonary infections, P. often encounters environments where the levels of calcium (Ca 2+ ) are elevated. Previously, we showed responds to externally added Ca through enhanced biofilm formation, increased production several secreted virulence factors, and by developing a transient...
Biofilms are differentiated microbial communities held together by an extracellular matrix. μCT X-ray revealed structured mineralized areas within biofilms of lung pathogens belonging to two distant phyla - the proteobacteria Pseudomonas aeruginosa and actinobacteria Mycobacterium abscessus. Furthermore, calcium chelation inhibited assembly complex bacterial structures for both organisms with little no effect on cell growth. The molecular mechanisms promoting calcite scaffold formation were...
Pseudomonas aeruginosa is a facultative human pathogen, and major cause of nosocomial infections severe chronic in endocarditis cystic fibrosis (CF) patients. Calcium (Ca2+) accumulates pulmonary fluids CF patients, plays role the hyperinflamatory response to bacterial infection. Earlier we showed that P. responds increased Ca2+ levels, primarily through production secreted virulence factors. Here describe putative Ca2+-binding protein, with an EF-hand domain, PA4107 (EfhP), this response....
Lately, there has been a special interest in understanding the role of halophilic and halotolerant organisms for their ability to degrade hydrocarbons. The focus this study was investigate genes enzymes involved initial steps benzene degradation pathway halophiles. extremely bacteria Arhodomonas sp. strain Seminole Rozel, which toluene as sole carbon source at high salinity (0.5 4 M NaCl), were isolated from enrichments developed contaminated hypersaline environments. To obtain insights into...
Rhodococcus jostii RHA1, a catabolically diverse soil actinomycete, is highly resistant to long-term nutrient starvation. After 2 years of carbon starvation, 10% the bacterial culture remained viable. To study molecular basis such resistance, we monitored abundance about 1,600 cytosolic proteins during 2-week period source (benzoate) Hierarchical cluster analysis elucidated 17 major protein clusters and showed that most changes occurred transition stationary phase. We identified 196...
Bacterial β-class carbonic anhydrases (CAs) are zinc metalloenzymes catalysing reversible hydration of CO2. They maintain the intracellular balance CO2/bicarbonate required for biosynthetic reactions and represent a new group antimicrobial drug targets. Genome sequence analysis Pseudomonas aeruginosa PAO1, an opportunistic human pathogen causing life threatening infections, identified three genes, PAO102, PA2053 PA4676, encoding putative β-CAs that share 28-45 % amino acid identity belong to...
Antimicrobial resistance (AMR) poses a significant worldwide public health crisis that continues to threaten our ability successfully treat bacterial infections. With the decline in effectiveness of conventional antimicrobial therapies and lack new antibiotic pipelines, there is renewed interest exploring potential metal-based compounds. Antimony-based compounds with long history use medicine have re-emerged as agents. We previously synthesized series novel organoantimony(V) complexed...
Ciprofloxacin (CPX) is one of the most employed antibiotics in clinics to date. However, rise drug-resistant bacteria dramatically impairing its efficacy, especially against life-threatening pathogens, such as
Pseudomonas aeruginosa is a Gram-negative facultative anaerobe belonging to the Pseudomonadaceae family. It multidrug-resistant opportunistic human pathogen, common cause of life-threatening nosocomial infections, and key bacterial agent in cystic fibrosis endocarditis. The bacterium exhibits intrinsic resistance most antibacterial agents, including aminoglycosides quinolones. Hence, identification new drug targets for P. ongoing. PsCA3 β-class carbonic anhydrase (β-CA) that catalyzes...
The DedA superfamily is a highly conserved family of membrane proteins. Deletion
Acetonitrile hydratase (ANHase) of Rhodococcus jostii RHA1 is a cobalt-containing enzyme with no significant sequence identity characterized nitrile hydratases. The ANHase structural genes anhA and anhB are separated by anhE, predicted to encode an 11.1-kDa polypeptide. An anhE deletion mutant did not grow on acetonitrile but grew acetamide, the reaction product. Growth was restored providing in trans. AnhA could be used assemble vitro, provided growth medium supplemented 50 microM CoCl(2)....
Biofilms are surface-associated microbial communities known for their increased resistance to antimicrobials and host factors. This introduces a critical clinical challenge, particularly in cases associated with implants increasing the predisposition bacterial infections. Preventing such infections requires development of novel or compounds that enhance bactericidal effect currently available antibiotics. We have synthesized characterized twelve silver(I) cyanoximates designated as Ag(ACO),...