A5067968674- Wound Healing and Treatments
- Meat and Animal Product Quality
- Aquaculture Nutrition and Growth
- Corneal Surgery and Treatments
- Heavy metals in environment
- Plant Micronutrient Interactions and Effects
- Plant Stress Responses and Tolerance
- Cancer Cells and Metastasis
- Seaweed-derived Bioactive Compounds
- Algal biology and biofuel production
- Hair Growth and Disorders
- Sustainable Supply Chain Management
- Coastal and Marine Management
- Polysaccharides Composition and Applications
- Skin and Cellular Biology Research
- Microbial Metabolites in Food Biotechnology
- Microplastics and Plastic Pollution
- Silk-based biomaterials and applications
- Botanical Research and Applications
- Cellular Mechanics and Interactions
- Food composition and properties
- Protein Hydrolysis and Bioactive Peptides
- Sensory Analysis and Statistical Methods
- Proteins in Food Systems
- Fish Biology and Ecology Studies
Instituto Politécnico de Leiria
2020-2024
MARE - Centro de Ciências do Mar e do Ambiente
2023-2024
Boston University
2017-2023
Aquatic Systems (United States)
2023
University of Lisbon
2015-2019
California Institute for Regenerative Medicine
2013
University of Pennsylvania
2013
Harvard University
2007
Direct conversion of fibroblasts to induced cardiomyocytes (iCMs) has great potential for regenerative medicine. Recent publications have reported significant progress, but the evaluation reprogramming relied upon non-functional measures such as flow cytometry cardiomyocyte markers or GFP expression driven by a cardiomyocyte-specific promoter. The issue is one practicality: most stringent - electrophysiology detect cell excitation and presence spontaneously contracting myocytes are not...
In this work we studied the effect of cadmium (Cd) (25 μM), in spinach plants (Spinacea oleracea) growing nutrient solution, for 1, 2 and 7 days. Spinach contaminated solution showed a decrease biomass, chlorophyll content an increase malondialdehyde (MDA) content, showing that photosynthetic apparatus was affected lipid peroxidation occurred. The main defence mechanisms against induced oxidative stress were activation catalase, glutathione reductase guaiacol peroxidase. Glutathione activity...
Abstract Storing non‐marketable fish aboard fishing vessels takes up valuable storage space and, consequently, it is usually discarded. Therefore, the strategy to minimize amount of discarded and boost economy should entail measures turn unexploited species into marketable fish. The use sensory descriptive analysis first step in characterization foods assess their baseline appeal final consumer. In this study, five was conducted, using a semi‐trained panel, with objective establishing...
Summary Algae and field crops can innovate improve pasta; however, incorporation of high‐fibre materials weaken the protein‐starch matrix. This study's aim was to verify how ratios xanthan gum (XG) linseed flour (LF) would influence physical, sensory attributes bioactivity pasta supplemented with algae. Fucus vesiculosus Chlorella vulgaris were incorporated into durum wheat along LF or XG. Samples evaluated for optimal cooking time (OCT), loss, colour variation, texture profile analysis...
To preserve limbal stem cell (LSC) function in vitro with xenobiotic-free culture conditions.Limbal epithelial cells were isolated from 139 donors using 15 variations of three dissociation solutions. All conditions compared to the baseline condition murine 3T3-J3 feeders xenobiotic (Xeno) keratinocyte growth medium at 20% O2. Five Xeno and Xeno-free media increasing concentrations calcium epidermal factor (EGF) evaluated 5%, 14%, Human MRC-5, dermal (fetal, neonatal, or adult), stromal...
Mouse models have been used to provide primary cells study physiology and pathogenesis of epithelia. However, highly efficient simple approaches propagate mouse epithelial remain challenging. Here, we show that pharmacological inhibition TGF-β signaling enables long-term expansion p63+ progenitor in low Ca2+ media without the need cell-purification steps or support by a feeder cell layer. We find is operative keratinocytes conventional cultures as determined nuclear Smad2/3 localization....
The most consumed fish species in Portugal do not correspond to the caught on Portuguese coast. From point of view ocean sustainability and economics, it is important study unexploited or underexploited that have potential be introduced into food market. This can reflected by their nutritional value, where mineral elements are often overlooked despite importance human health. Therefore, elemental concentration those species, which should also include seasonal variations essential such as Ca,...
We live in a world of limited biological resources and ecosystems, which are essential to feed people. Consequently, diversifying target species considering full exploitation for fishery sustainability. The present study focuses on the valorization three low commercial value fish (blue jack mackerel, Trachurus picturatus; black seabream, Spondyliosoma cantharus; piper gurnard, Trigla lyra) two unexploited (comber, Serranus cabrilla boarfish, Capros aper) through development marine-based food...
Abstract Target species diversification is essential for fisheries sustainability and fish market revitalization. Fish discards are a widely recognized problem resulting from worldwide, of major concern all sector players, administrations, to fishermen, scientists. However, non‐target seldom studied, information on nutritional profiles seasonal changes in properties generally lacking. This study assessed the composition two unexploited ( Serranus cabrilla , Capros aper ) three low commercial...
Abstract Modern consumers demand the replacement of synthetic colorants with natural alternatives. Microalgae can serve as an alternative source for these since they hold significant amounts pigments. This study aimed to evaluate potential using microalgae biomass and extracts pastry confectionary products. The application different levels Chlorella vulgaris (White, Honey, a mixture both) was evaluated in brioche‐type breads egg substitute confer typical yellow coloration product. A 1%...
Abstract The surface of the middle ear is composed tympanic membrane (TM) and mucosa (MEM). A number diseases conditions such as otitis media, cholesteatoma, perforation TM have been reported to cause dysfunction ear, ultimately leading high-frequency hearing loss. Despite its importance in repairing damaged tissues, stem/progenitor cells MEM epithelia remains largely uncharacterized due, part, lack an optimal methodology expand maintain long-term. Here, we show that suppression TGF-β...
What consumers choose when purchasing food is of most importance to promote sustainability. The consumption more sustainable foods should be stimulated, for example, by using ingredients and consumer education. Therefore, an innovative highly nutritious vegan burger with seaweed (VBS) was developed ingredients, such as pulses—grass pea (Lathyrus sativus L.) chickpea (Cicer arietinum L.)—and the Dulse (Palmaria palmata from aquaculture. VBS analysed its physico-chemical nutritional...
Cultured epidermal autografts have been used worldwide since 1981 for patients with extensive third-degree burn wounds and limited skin donor sites. Despite significant progress in techniques toward improving clinical outcome of grafts, the long vitro preparation time cultured has remained a major factor limiting its widespread use. Here, we show that pharmacological inhibition TGF-β signaling promotes expansion human keratinocytes (HEKs) high proliferative potential co-cultures both murine...
Overfishing is increasing over time, and according to FAO (Food Agriculture Organization), about one third of the world’s fish stocks are now overfished. Thus, diversifying target species essential for fisheries sustainability contributing improve resource-efficient processes. Non-target can be valuable resources development new food products. However, those scarcely studied, it high importance trace their seasonal sensory profile as a first step towards valorisation. Therefore, in this...
In this work the effect of addition different amounts cattle slurry (CS) to a Cd contaminated soil, was studied regarding its in spinach plants. Two levels contamination (2 and 10 mg/kg) three CS were evaluated (2.5, 5 g CS/100 soil). Spinach shown be tolerant species, able accumulate relatively high (up 367.7 mg/kg leaves), exceeding limits established by European regulations for leaf vegetables. The 2.5 soil containing 2 did not reduce uptake metal but allowed plants grow as much control....
In this study, different extraction methods and conditions were used for the of antioxidants from brown macroalgae Fucus spiralis. The methodologies ultrasound-assisted (ultrasonic bath ultrasonic probe), with a vortex, an Ultra-Turrax® homogenizer, high-pressure-assisted extraction. extracts analyzed their total phenolic content (TPC) antioxidant activity, evaluated through 2,2-difenil-1-picrilhidrazil (DPPH) free radical scavenging method ferric reducing power (FRAP) assay. Ultrasonic...
Pastry products constitute a significant segment of the food market. However, high amount fat used in their production poses challenge when competing for attention modern consumers, who are more conscious health problems associated with consumption high-fat products. With this mind, main objective study is reduction total and saturated contents two bakery products, brioche-type bread rice cake, by partial substitution source