A5031335375- Seaweed-derived Bioactive Compounds
- Marine Sponges and Natural Products
- Algal biology and biofuel production
- Protein Hydrolysis and Bioactive Peptides
- Microbial Metabolites in Food Biotechnology
- Microbial Natural Products and Biosynthesis
- Bioactive Compounds and Antitumor Agents
- Food Industry and Aquatic Biology
- Nanocomposite Films for Food Packaging
- Viral Infectious Diseases and Gene Expression in Insects
- Biosimilars and Bioanalytical Methods
- Phagocytosis and Immune Regulation
- Aquatic life and conservation
- Concrete and Cement Materials Research
- Fluid Dynamics and Mixing
- Marine Bivalve and Aquaculture Studies
- Echinoderm biology and ecology
- Food and Agricultural Sciences
- Synthesis and biological activity
- Aquaculture disease management and microbiota
- Phytoestrogen effects and research
- Marine and coastal plant biology
- Microbial Inactivation Methods
- Drilling and Well Engineering
- Cell death mechanisms and regulation
Instituto Politécnico de Leiria
2013-2024
Portuguese Sea and Atmosphere Institute
2021-2024
University of Lisbon
2021-2024
Universidade Federal de São Carlos
2015
Instituto Politécnico Nacional
2015
Chitin was extracted from Polybius henslowii, a swimming crab, captured in large quantities throughout the Portuguese coast by purse seine vessels as bycatch. After standard chitin extraction procedures, water-soluble chitosan products were obtained via two different methods: (1) N-acetylation with addition of acetic anhydride and (2) reaction hydrogen peroxide. The chemical structure molecular weight derivatives, (WSC) chitooligosaccharides (COS), confirmed Fourier Transform Infrared...
Cancer and infectious diseases continue to be a major public health problem, new drugs are necessary. As marine organisms well known provide wide range of original compounds, the aim this study was investigate bioactivity main constituents cosmopolitan red alga, Sphaerococcus coronopifolius. The structure several bromoditerpenes determined by extensive spectroscopic analysis comparison with literature data. Five molecules were isolated characterized which include brominated diterpene...
Surface-associated marine bacteria are an interesting source of new secondary metabolites. The aim this study was the isolation and identification epiphytic from brown alga, Bifurcaria bifurcata, evaluation antioxidant antimicrobial activity extracts. determined by 16S rRNA gene sequencing. Bacteria extracts were obtained with methanol dichloromethane (1:1) extraction. performed quantification total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging oxygen...
Summary The aim of this work was to study the proximate composition and bioactive profile Bifurcaria bifurcata . It contains 73.31 ± 0.69% moisture, 8.57 0.11 g per 100 dry weight (d.w.) protein, 5.81 0.14 d.w. lipid content 30.15 0.00 ash. polyunsaturated fatty acids were most abundant acid ( FA ), accounting for 2426.56 mg which represents 41.77% total methanolic fraction showed high quantity polyphenols (220.01 0.010 phloroglucinol equivalents −1 extract), DPPH radical reduction capacity...
Cancer represents a serious threat for human health with high social and economic impacts worldwide. Therefore, the development of new anticancer drugs is most importance. The aim present study was to evaluate antitumor potential twelve algae from Portugal coast on an in vitro model hepatocellular carcinoma (HepG-2 cells).Both extracts Asparagopsis armata (1000 µg/ml; 24 h) presented cytotoxicity 11.22 ± 2.98 1.51 0.38 % HepG-2 live cells, respectively. Sphaerococcus coronopifolius...
Fucus spiralis is an edible brown seaweed (SW) found in the Portuguese Coast. It has been reported to have high antioxidant activity, which may elicit a potential use for food industry. However, little information available on how SW behaves during digestive process and freeze-drying might affect bioaccessibility of different compounds. Therefore, total polyphenols, lipid, fatty acid contents were measured before after vitro simulation human process, both fresh freeze-dry SW. F. had lipid...
Antioxidants play an important role as Reactive Oxygen Species (ROS) chelating agents and, therefore, the screening for potent antioxidants from natural sources potential protective is of great relevance. The main aim this study was to obtain antioxidant-enriched fractions common seaweed Fucus spiralis and evaluate their activity efficiency in protecting human cells (MCF-7 cells) on oxidative stress condition induced by H2O2. Five fractions, F1–F5, were obtained reversed-phase vacuum liquid...
The marine environment has shown to be an interesting source of new antitumor agents, representing important tool in cancer research.The aim this study was evaluate the activities 12 algae from Peniche coast (Portugal) on vitro model human colorectal (Caco-2 cells).The potential accessed by evaluating Caco-2 cell's viability and proliferation through 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide calcein-AM methods.The dichloromethane extracts Asparagopsis armata...
Due to the high consumption of fat-rich processed foods, efforts are being done reduce their saturated fat (SFA) contents and replace it with polyunsaturated fatty acids (PUFA), creating a necessity find alternative PUFA sources. Macroalgae, promising natural source healthy food, may be such an alternative. The acid (FA) profile Fucus spiralis, Bifurcaria bifurcata, Ulva lactuca, Saccorhiza polyschides were determined through direct transesterification seasonal variation was studied. F....
Abstract Macroalgae‐associated bacteria have already proved to be an interesting source of compounds with therapeutic potential. Accordingly, the main aim this study was characterize Asparagopsis armata ‐associated community and evaluate their capacity produce substances antitumor antimicrobial Bacteria were selected according phenotype isolated by streak plate technique. The identification carried out RNA ribosomal 16s gene amplification through PCR techniques. activities evaluated against...
In this study, different extraction methods and conditions were used for the of antioxidants from brown macroalgae Fucus spiralis. The methodologies ultrasound-assisted (ultrasonic bath ultrasonic probe), with a vortex, an Ultra-Turrax® homogenizer, high-pressure-assisted extraction. extracts analyzed their total phenolic content (TPC) antioxidant activity, evaluated through 2,2-difenil-1-picrilhidrazil (DPPH) free radical scavenging method ferric reducing power (FRAP) assay. Ultrasonic...
Diversas proteínas são produzidas em Escherichia coli recombinante empregando reatores convencionais (tanque agitado e aerado), mas há poucos estudos envolvendo o biorreator airlift, cujas vantagens são: menor complexidade de construção, baixo risco contaminação maior eficiência energética na transferência oxigênio. Devido ao alto consumo oxigênio característico dessa bactéria, a operação sobrepressão se apresenta como um recurso para compensar no airlift. Nesse contexto, objetivou-se...
O biorreator airlift apresenta vantagens frente aos reatores convencionais (tanque agitado e aerado), como menor complexidade de construção, baixo risco contaminação maior eficiência energética na transferência oxigênio. Entretanto, a oxigênio no airlift, em relação à alcançada reator convencional, justifica pressurização do tanque fim maximizar esse transporte massa. Neste âmbito, o objetivo deste trabalho foi avaliar influência da pressão sobre retenção gasosa (ɛ) coeficiente volumétrico...