A5032374053- Hearing, Cochlea, Tinnitus, Genetics
- Hearing Loss and Rehabilitation
- Biochemical Analysis and Sensing Techniques
- Bat Biology and Ecology Studies
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Neuroscience and Neural Engineering
- Acoustic Wave Phenomena Research
- Noise Effects and Management
- Plant and Biological Electrophysiology Studies
- Analog and Mixed-Signal Circuit Design
Johns Hopkins Medicine
2021-2024
Johns Hopkins University
2021-2024
University Medical Center Groningen
2016-2020
University of Groningen
2018-2020
Type I spiral ganglion neurons (SGNs) are the auditory afferents that transmit sound information from cochlear inner hair cells (IHCs) to brainstem. These consist of physiological subtypes differ in their spontaneous firing rate (SR), activation threshold, and dynamic range have been described as low, medium, high SR fibers. Lately, single-cell RNA sequencing experiments revealed three molecularly defined type SGN subtypes. The extent which correspond is unclear. To address this question, we...
Abstract Potassium (K + ) channels shape the response properties of neurons. Although enormous progress has been made to characterize K in primary auditory neurons, molecular identities many these and their contributions hearing vivo remain unknown. Using a combination RNA sequencing single molecule fluorescent situ hybridization, we localized expression transcripts encoding sodium-activated potassium Na 1.1 (SLO2.2/Slack) 1.2 (SLO2.1/Slick) neurons (spiral ganglion SGNs). To examine...
In different animal models, auditory nerve fibers display variation in spontaneous activity and response threshold. Functional structural differences among inner hair cell ribbon synapses are believed to contribute this variation. The relative volumes of synaptic proteins at individual might be one such difference. This idea is based on the observation opposing volume gradients presynaptic ribbons associated postsynaptic glutamate receptor patches mice along pillar modiolar axis cell, same...
Presbycusis is a prevalent condition in older adults characterized by the progressive loss of hearing due to age-related changes cochlea, auditory portion inner ear. Many also struggle with understanding speech noise despite having normal thresholds, termed "hidden" because it evades standard audiological assessments. Examination animal models and postmortem human tissue suggests that hidden associated cochlea may, therefore, precede overt loss. Nevertheless, pathological mechanisms...
Compared to many other rodent species, naked mole rats (Heterocephalus glaber) have elevated auditory thresholds, poor frequency selectivity, and limited ability localize sound. Because the cochlea is responsible for encoding relaying signals brain, we used immunofluorescence quantitative image analysis examine cochlear innervation in mature developing compared mice (Mus musculus), gerbils (Meriones unguiculatus), Damaraland (Fukomys damarensis), another subterranean rodent. In comparison...
Approaches to identify the perception of tinnitus in various animal models have been difficult apply mouse. As a result, mice underutilized investigate cellular, molecular, and genetic mechanisms underlying tinnitus. A recent study guinea pigs identified novel spontaneous behavior (unconditioned response), changes movement during silent gaps, that subgroup animals presumably with Guinea failed "freeze" response gaps sound. In hope developing rapid reliable assay for mice, we used similar...
This protocol describes the preparation of mouse organ Corti for RNAscope, immunolabeling, confocal microscopy, and quantitative image analysis to examine transcript protein localization, sensory hair cells, synapses. can be applied mice other rodents (juvenile adult) adapted techniques, including electrophysiology RNA sequencing. features minimal tissue processing preserve viability downstream assays, while isolating is most challenging step. For additional details on use execution this...