A5039358683- Ion Channels and Receptors
- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Inflammasome and immune disorders
- IL-33, ST2, and ILC Pathways
- Peptidase Inhibition and Analysis
- Glutathione Transferases and Polymorphisms
- Cardiovascular Disease and Adiposity
- Neuropeptides and Animal Physiology
- Eosinophilic Esophagitis
- Genomics, phytochemicals, and oxidative stress
- Influenza Virus Research Studies
- Inflammatory Bowel Disease
- Immunotherapy and Immune Responses
- Pain Mechanisms and Treatments
- Phytochemicals and Antioxidant Activities
- Urinary Bladder and Prostate Research
- Agricultural Practices and Plant Genetics
- Antioxidant Activity and Oxidative Stress
- Curcumin's Biomedical Applications
- Inhalation and Respiratory Drug Delivery
- Vitamin C and Antioxidants Research
- Immune Cell Function and Interaction
- Gut microbiota and health
- Cholesterol and Lipid Metabolism
University of Tasmania
2011-2021
Launceston General Hospital
2016-2017
Ingham Institute
2016
Western Sydney University
2016
UNSW Sydney
2016
RMIT University
2014
MCC950 a potent, highly specific small molecule inhibitor of canonical and noncanonical activation NLRP3 inflammasome has been evaluated in multitude driven inflammatory diseases. However, the effect on colonic inflammation not yet reported. In present study we investigated spontaneous chronic colitis mouse model Winnie, which mimics human ulcerative colitis. Oral administration 40 mg/kg commencing at Winnie week seven for three weeks significantly improved body weight gain, colon length, to...
A contributing factor in the development of ulcerative colitis (UC) and Crohn's disease (CD) is disruption innate adaptive signaling pathways due to aberrant cytokine production. The cytokine, interleukin (IL)-1β, highly inflammatory its production tightly regulated through transcriptional control both inflammasome-dependent inflammasome- independent proteolytic cleavage. In this study, qRT-PCR, immunohistochemistry, immunofluorescence confocal microscopy were used (1) assess mRNA expression...
Oxidative stress has been linked to the progression of disease, including chronic kidney disease (CKD). The aim present study was determine association between single-nucleotide polymorphisms (SNPs) antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase their activities CKD.This is a prospective cohort 185 CKD patients (Stages 2-4), followed for up 12 months. All were genotyped SNPs SOD (SOD Ala16Val), GPx (GPx Pro197Leu) (C-262T). rate change over period...
Inflammatory bowel disease (comprising ulcerative colitis and Crohn's disease) is a multifactorial that extensively associated with stool microbiome changes (dysbiosis). Appendicitis appendectomy limits subsequent colitis, clinically, in animal models. We wanted to examine how the appendiceal fared our spontaneous colitic Winnie (Muc2(-/-)) mice model.Two C57BL/6 10 at ages 12 15 weeks were euthanized for caecal patch samples. DNA was extracted using QIAamp Stool Mini Kit then V1-V3...
Fruits and vegetables are rich in plant polyphenols, whose consumption is encouraged healthy dietary regimes due to their antioxidants anti-inflammatory effects. These organic molecules exhibit numerous properties including phylochelation; the ability complex metal ions, highly reactive iron. Among we focused our attention on quercetin that previously demonstrated its reduce dendritic cells (DCs) inflammatory cytokine secretion antigen presentation following LPS exposure. Dendritic cell...
Abstract Inflammatory bowel disease (Crohn’s (CD) and ulcerative colitis (UC)) is a multifactorial resulting from immune dysregulation in the gut. The underlying characterized by high levels of inflammatory cytokines, including TNF α . Biological intervention for IBD patients using anti-TNF antibodies often an effective therapeutic solution. However, neutralization fails to induce remission subgroup patients, primarily UC patients. There dearth suitable animal models representing...
The oxidation of low-density lipoprotein (LDL) is believed to be the initiating factor for development and progression atherosclerosis. active ingredients spices such as chili turmeric (capsaicin curcumin, respectively) have been shown reduce susceptibility LDL oxidation. One techniques used study isolate subject it metal-induced (copper or iron) However, whole serum may represent a closer situation in vivo conditions than using isolated LDL. We investigated effects different concentrations...
Dendritic cells (DCs) are professional antigen presenting (APCs) that in response to microbial infections generate long-lasting adaptive immune response. Following uptake, DCs undergo a cascade of cellular differentiation ultimately leads "mature" DCs. Mature produce variety inflammatory cytokines, including tumor necrosis factor-α (TNFβ) key cytokine for the cascade. In numerous studies, polyphenols, quercetin, demonstrated their ability suppress TNFα secretion and protect from onset...
Currently little is known as to how nutritionally derived compounds may affect dendritic cell (DC) maturation and potentially prevent inappropriate inflammatory responses that are characteristic of chronic syndromes. Previous observations have demonstrated two polyphenols quercetin piperine delivered through reconstituted oil bodies (ROBs-QP) can influence DC in response LPS leading a modulated response. In the present study, we examined molecular effects ROBs-QP exposure on differentiation...
A contributing factor in the development of ulcerative colitis (UC) and Crohn's disease (CD) is aberrant signaling innate immune complex known as inflammasome. The inflammasome regulates production interleukin (IL)1β drives downstream inflammatory pathways. In this study human tissue (ethics approval: H11930), we provide evidence for disease-specific up-regulation NLRP6 transcript protein ileal CD describe an NLRP6-expressing goblet cell (GC) located predominantly upper portion intestinal crypt.
Oxidative stress is associated with the progression of chronic kidney disease (CKD). Links between antioxidant enzyme SNPs such as superoxide dismutase (SOD) Ala16Val, glutathione peroxidase (GPx) Pro197Leu and catalase C- 262T CKD have not been investigated. This study compared genotypes activities patients population controls, determined their relationship to function.CKD (n = 230) controls 224) were screened for GPx, SOD SNPs. Plasma red blood cell (RBC) RBC activities, estimated...
Bitter Melon (BM) has been used as a functional food in traditional Chinese and Indian medicine for many generations gained great deal of attention due to its apparent benefits moderating some the pathogenic processes variety inflammatory conditions. BM extract (BME) shown possess strong anti-oxidant properties. In addition, it can ameliorate oxidative stress potentially ER stress. There is increasing evidence that are major contributors intestinal secretory cell dysfunction which leads...
The effect of the plant-derived vanilloid, capsaicin (CAP), on metabolic activity THP-1, U266B1 and U937 hematological malignancy cells was determined. CAP reduced in a concentration-dependent manner three cell lines. A biphasic observed THP-1 (EC50: IC50 (95% CI) 32.9 (19.9-54.3)/219 (144-246) µmol/l). were more resistant to than U937. Metabolic significantly inhibited by compared (IC50: 197 versus 431 µmol/l, respectively, p < 0.008). Transient receptor potential vanilloid-1 (TRPV1) CB1...
To determine if exacerbation of pre-existing chronic colitis in Winnie (Muc2 mutant) mice induces colonic dysplasia.Winnie and C57BL6 as a genotype control, were administered 1% w/v dextran sulphate sodium (DSS) orally, followed by drinking water alone week-long cycles for total three cycles. After the third cycle, killed tissue collected histological immunohistochemical evaluation. Inflammation severity dysplasia mucosa assessed H&E sections colon. Epithelial cell proliferation was using...
Abstract Objectives The objective of this study was to determine the prevalence specific acquired macrolide resistance genes previously reported as present in clinical isolates Haemophilus influenzae. Methods A collection 172 respiratory H. influenzae, including 59 from cystic fibrosis patients and 27 non-cystic bronchiectasis with significant prior use, established. This tested for azithromycin susceptibility using Etest screened presence erm(A), erm(B), erm(C), erm(F), mef(A) mef(E) locked...
To investigate the phenotypic effect of expression selected acquired macrolide resistance genes (AMRGs) in non-typeable Haemophilus influenzae (NTHi).The AMRGs erm(A), erm(B) and erm(C) were cloned into Escherichia coli JM109 using shuttle vector pLS88; constructed plasmids extracted from suitable clones used to transform H. Rd by electroporation. Erythromycin azithromycin MICs for transformants determined broth microdilution. AMRG was quantitative PCR on transformant cDNA with locked...
Probiotics have been widely used in maintaining gastrointestinal health, despite their actual mechanism remaining obscure. There are several hypotheses behind the beneficial effects of probiotics including regulation intestinal barrier function and improvement immune responses system. Multiple introduced market as effective dietary supplements improving integrity, but there no or few studies that demonstrate underlying mechanism. In current study, we investigated compared efficacy four...
Nontypeable Haemophilus influenzae (NTHi) is a leading causative organism of opportunistic respiratory tract infections. However, there are currently no effective vaccination strategies, and existing treatments compromised by antibiotic resistance. We previously characterized haemolyticus (Hh) strains capable producing haemophilin (HPL), heme-binding protein that restricts NTHi growth limiting its access to an essential factor, heme. Thus, these may have utility as probiotic therapy against...