A5042870599- Protein Structure and Dynamics
- Enzyme Structure and Function
- Advanced NMR Techniques and Applications
- NMR spectroscopy and applications
- Protein Kinase Regulation and GTPase Signaling
- Molecular spectroscopy and chirality
- RNA and protein synthesis mechanisms
- Ion channel regulation and function
- Advanced MRI Techniques and Applications
- Bacterial Genetics and Biotechnology
- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Electron Spin Resonance Studies
- DNA and Nucleic Acid Chemistry
- Hemoglobin structure and function
- HIV Research and Treatment
- Glycosylation and Glycoproteins Research
- Cardiac electrophysiology and arrhythmias
- Chemical Synthesis and Analysis
- Spectroscopy and Chemometric Analyses
- Lipid Membrane Structure and Behavior
- RNA Research and Splicing
- Prion Diseases and Protein Misfolding
- Protist diversity and phylogeny
- Heat shock proteins research
University of Regensburg
2016-2025
Max Planck Institute for Medical Research
1994-2024
Max Planck Society
1994-2024
Czech Academy of Sciences, Institute of Biophysics
2009-2018
Fondazione Umberto Di Mario
2010
Goethe University Frankfurt
2001-2005
University of Freiburg
2003
University of Basel
2003
Ruhr University Bochum
2003
National Institute of Diabetes and Digestive and Kidney Diseases
2002
We have investigated the dynamic properties of switch I region GTP-binding protein Ras by using mutants Thr-35, an invariant residue necessary for function. Here we show that these mutants, previously used as partial loss-of-function mutations in cell-based assays, a reduced affinity to effector proteins without Thr-35 being involved any interaction. The structure Ras(T35S)(.)GppNHp was determined x-ray crystallography. Whereas overall is very similar wildtype, residues from are completely...
31P NMR revealed that the complex of p21ras with GTP analog GppNHp.Mg2+ exists in two conformational states, states 1 and 2. In wild-type equilibrium constant K1(12) between is 1.09. The population these different for various mutants but independent temperature. activation enthalpy delta H ++ entropy S transitions were determined by full-exchange matrix analysis p21ras(S65P). For protein one obtains = 89 +/- 2 kJ mol-1 102 20 J K-1 mutant 93 7 138 30 K-1. study suggests correspond to...
The guanine nucleotide-binding protein Ras exists in solution two different conformational states when complexed with GTP analogs such as GppNHp or GppCH2p. State 1 has only a very low affinity to effectors and seems be recognized by nucleotide exchange factors, whereas state 2 represents the high effector binding state. In this work we investigate complex physiological nucleoside triphosphate GTP. By polarization transfer 31P NMR experiments studies show that Ras(wt)·Mg2+·GTP also dynamical...
En route to new inhibitors: The binding of Zn2+ cyclen the human Ras protein stabilizes a conformation that has weak affinity for effectors. Consequently this complex is lead structure inhibition studies on Ras–effector interaction. picture shows NMR Ras⋅Mg2+⋅GppNHp complexed cyclen. Detailed facts importance specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted by authors. Please note:...
Folding and function of proteins are two aspects which usually considered as basically unrelated phenomena that optimized by evolution independently. From the funnel model folding/unfolding associated energy landscape, we infer paradigm minimum number folding intermediates is determined all functional states a protein ("essential" intermediates). Here, demonstrate supposed fundamental link using Ras complexed with GTP analogue GppNHp occurs in structural coexisting solution. State 2 was...
Liver sinusoidal endothelial cells (LSEC) are characterized by the presence of fenestrations that not bridged a diaphragm. The molecular mechanisms control formation largely unclear. Here we report mice, which deficient in plasmalemma vesicle-associated protein (PLVAP), develop distinct phenotype is caused lack fenestrations. Fenestrations with diaphragm were observed mouse LSEC at three weeks age, but present during embryonic life starting from day 12.5. PLVAP was expressed wild-type...
Cellular signaling downstream of Ras is highly diversified and may involve many different effector molecules. A potential candidate AF6 which was originally identified as a fusion to ALL-1 in acute myeloid leukemia. In the present work interaction between characterized compared with other effectors. The binding characteristics are quite similar Raf RalGEF, <i>i.e.</i> nucleotide dissociation well GTPase-activating protein activity inhibited, whereas intrinsic GTPase unperturbed by binding....
Dehydration stress in the drought-tolerant resurrection plant Craterostigma plantagineum is accompanied by accumulation of a large number desiccation proteins (Dsp). One abundant class these represented dehydrin-related Dsp16 protein which contains 15 amino acid conserved lysine-rich repeats and stretch eight serine residues providing extremely hydrophilic characteristics. Recombinant from has been cloned expressed Escherichia coli. The was purified characterized regarding its...
Cold‐shock proteins (Csps) are a subgroup of the cold‐induced preferentially expressed in bacteria and other organisms on reduction growth temperature below physiological temperature. They related to cold‐shock domain found eukaryotes some most conserved known. Their exact function is still not known, but translational regulation, possibly via RNA chaperoning, has been discussed. Here we present structure hyperthermophilic member Csp family. The NMR solution Tm from Thermotoga maritima ,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThree-dimensional structures and properties of a transforming nontransforming glycine-12 mutant p21H-rasSybille M. Franken, Axel J. Scheidig, Ute Krengel, Hans Rensland, Alfred Lautwein, Matthias Geyer, Klaus Scheffzek, Roger S. Goody, Robert Kalbitzer, Cite this: Biochemistry 1993, 32, 33, 8411–8420Publication Date (Print):August 24, 1993Publication History Published online1 May 2002Published inissue 24 August...
Enzyme-IIIglc is part of the glucose phosphotransferase system Escherichia coli and Salmonella typhimurium phosphorylated by phosphoenolpyruvate in a reaction requiring enzyme I (phosphoenolpyruvate-protein phosphotransferase), histidine-containing phospho-carrier protein HPr. In this paper we report isolation IIIglc from E. characterization active center. Alkaline hydrolysis [32P]P-IIIglc chromatography hydrolysate suggested that phosphoryl group bound to histidyl residue P-IIIglc S....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHPr proteins of different microorganisms studied by proton-high-resolution nuclear magnetic resonance: similarities structures and mechanismsH. R. Kalbitzer, W. Hengstenberg, P. Roesch, Muss, Bernsmann, Engelmann, M. Doerschug, J. DeutscherCite this: Biochemistry 1982, 21, 12, 2879–2885Publication Date (Print):June 8, 1982Publication History Published online1 May 2002Published inissue 8 June...
A new type of allosteric inhibition by small molecules is proposed that should be applicable to all proteins involved intrinsically in protein–protein interactions. It based on targeting their rare interaction states can detected high-pressure NMR spectroscopy (see picture). An example the Ras-protein where Ras with effectors modulated compounds bind conformational 1(T) or 1(0).