A5037981388- Nanoparticle-Based Drug Delivery
- Advanced Drug Delivery Systems
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Hydrogels: synthesis, properties, applications
- Protein purification and stability
- Polymer Surface Interaction Studies
- Electrospun Nanofibers in Biomedical Applications
- Virus-based gene therapy research
- 3D Printing in Biomedical Research
- Ocular Surface and Contact Lens
- Gold and Silver Nanoparticles Synthesis and Applications
- Mesenchymal stem cell research
- Wound Healing and Treatments
- Retinal Diseases and Treatments
- Lipid Membrane Structure and Behavior
- Advancements in Transdermal Drug Delivery
- Osteoarthritis Treatment and Mechanisms
- Periodontal Regeneration and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Dendrimers and Hyperbranched Polymers
- Advanced Polymer Synthesis and Characterization
- Immunotherapy and Immune Responses
- Surfactants and Colloidal Systems
- Corneal Surgery and Treatments
University of Regensburg
2016-2025
Case Western Reserve University
2017
Cornell University
2017
Pharmaceutical Biotechnology (Czechia)
2010-2013
Pharma Stulln (Germany)
2013
University of Würzburg
2013
University Hospital Regensburg
2013
Charité - Universitätsmedizin Berlin
2013
University of Pennsylvania
2003
Massachusetts Institute of Technology
1995
Nonviral nucleic acid delivery to cells and tissues is considered a standard tool in life science research. However, although an ideal system should have high efficacy minimal toxicity, existing materials fall short, most of them being either too toxic or little effective. We hypothesized that disulfide cross-linked low-molecular-weight (MW) linear poly(ethylene imine) (MW<4.6 kDa) would overcome this limitation. Investigations with these revealed the extracellular MW provided outstandingly...
Although uptake into cells is highly complex and regulated, heterogeneous particle collectives are usually employed to deliver small interfering RNA (siRNA) cells. Within these collectives, it difficult accurately identify the active species, a decrease in efficacy inherent such preparations. Here, we demonstrate manufacture of uniform nanoparticles with deposition siRNA on gold layer-by-layer approach, further report cellular delivery activity as functions surface properties.
The Diels–Alder (DA) reaction was investigated as a cross-linking mechanism for poly(ethylene glycol) (PEG) based hydrogels. Two complementary macromonomers were synthesized by functionalizing star-shaped PEG with furyl and maleimide groups. Gel formation occurred in water at 37 °C; the gelation time ranged between 171 ± 25 min 14 1 depending on used hydrogel formulation. complex shear modulus dependent concentration, branching factor molecular weight of macromonomers; values 2821 1479 Pa...
Although there are a large number of studies available for the evaluation therapeutic efficacy targeted polymeric nanoparticles, little is known about critical attributes that can further influence their uptake into target cells. In this study, varying cRGD ligand densities (0-100% surface functionalization) were combined with different poly(ethylene glycol) (PEG) spacer lengths (2/3.5/5 kDa), and specific receptor binding core-shell structured poly(lactic- co-glycolic acid)/poly(lactic...
Linear polyethylenimine (LPEI) with a molecular weight (MW) of 22 kDa has been described as having superior ability to induce gene transfer compared its branched form. However, the transfection efficiency polymer cannot be enhanced beyond certain limit due cytotoxicity. We explored potential utilizing LPEIs MWs ranging from 1.0 9.5 overcome this limitation.Polyplexes plasmid DNA encoding for green fluorescent protein (EGFP) and various were concerning their cytotoxicity in CHO-K1 HeLa cells...
The use of either a hydrogel or solid polymeric scaffold alone is often associated with distinct drawbacks in many tissue engineering applications. Therefore, this study, we investigated the potential combination long-term stable fibrin gels and polyurethane scaffolds for cartilage engineering. Primary bovine chondrocytes were suspended gel subsequently injected into polycaprolactone-based scaffold. Cells homogeneously distributed within composite system produced high amounts...
Despite the clinical need for reconstructive and plastic surgery, supply of engineered adipose tissue equivalents still remains a challenge. As yet, only preadipocytes have been applied as cell material in vitro engineering fat. Herein, we report establishment three-dimensional (3-D) long-term culture, using bone marrow-derived mesenchymal stem cells (MSCs) an alternative source custom-made poly(lactic-co-glycolic acid) (PLGA) scaffolds carrier. Cell-polymer constructs were cultivated 4...
Abstract Because nanoparticles are finding uses in myriad biomedical applications, including the delivery of nucleic acids, a detailed knowledge their interaction with biological system is utmost importance. Here size‐dependent uptake gold (AuNPs) (20, 30, 50 and 80 nm), coated layer‐by‐layer approach acid poly(ethylene imine) (PEI), into variety mammalian cell lines studied. In contrast to other studies, optimal particle diameter for cellular determined but also number therapeutic cargo...
Formation of a protein corona can severely compromise the performance functionalized polymeric nanoparticles by changing their identity and integrity.
Liver sinusoidal endothelial cells (LSEC) are characterized by the presence of fenestrations that not bridged a diaphragm. The molecular mechanisms control formation largely unclear. Here we report mice, which deficient in plasmalemma vesicle-associated protein (PLVAP), develop distinct phenotype is caused lack fenestrations. Fenestrations with diaphragm were observed mouse LSEC at three weeks age, but present during embryonic life starting from day 12.5. PLVAP was expressed wild-type...
Eight-armed poly(ethylene glycol) was functionalized with furyl and maleimide groups. The two macromonomers were cross-linked by Diels-Alder (DA) reactions the degradation behavior of formed hydrogels investigated. UV spectroscopy showed that groups subject to ring-opening hydrolysis above pH 5.5, reaction rate depending on temperature. As a result this, gelation kinetics stiffness DA dependent temperature cross-linking medium, as demonstrated rheological experiments. gel time varied between...
Mesenchymal stem cells (MSCs) are capable of differentiating into a variety lineages, including bone, cartilage, or fat, depending on the inducing stimuli and specific growth differentiation factors. It is widely acknowledged that basic fibroblast factor (bFGF) modulates chondrogenic osteogenic MSCs, but thorough investigations its effects adipogenic lacking. In this study, we demonstrate cellular molecular level supplementation bFGF in different phases cell culture leads to strong...