A5044812464- 3D Printing in Biomedical Research
- Osteoarthritis Treatment and Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Mesenchymal stem cell research
- RNA Interference and Gene Delivery
- Periodontal Regeneration and Treatments
- Protein purification and stability
- Additive Manufacturing and 3D Printing Technologies
- Hydrogels: synthesis, properties, applications
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- Knee injuries and reconstruction techniques
- Advanced Drug Delivery Systems
- Lipid Membrane Structure and Behavior
- Polymer Surface Interaction Studies
- Proteoglycans and glycosaminoglycans research
- biodegradable polymer synthesis and properties
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Nanoparticle-Based Drug Delivery
- Cancer Cells and Metastasis
- Silk-based biomaterials and applications
- Blood properties and coagulation
- Innovative Microfluidic and Catalytic Techniques Innovation
- Retinal and Macular Surgery
Universitätsklinikum Würzburg
2016-2025
University of Würzburg
2014-2023
Klinik und Poliklinik für Mund-, Kiefer- und Plastische Gesichtschirurgie
2019
University Hospital Heidelberg
2018
Heidelberg University
2018
Kiel University
1992-2012
University of Regensburg
2001-2010
University Hospital Regensburg
2006
Massachusetts Institute of Technology
1996-2002
Freie Universität Berlin
1996
Bioprinting can be defined as the art of combining materials and cells to fabricate designed, hierarchical 3D hybrid constructs. Suitable materials, so called bioinks, have comply with challenging rheological processing demands rapidly form a stable hydrogel postprinting in cytocompatible manner. Gelatin is often adopted for this purpose, usually modified (meth-)acryloyl functionalities postfabrication curing by free radical photopolymerization, resulting that cross-linked via nondegradable...
The electrohydrodynamic stabilization of direct-written fluid jets is explored to design and manufacture tissue engineering scaffolds based on their desired fiber dimensions. It demonstrated that melt electrowriting can fabricate a full spectrum various fibers with discrete diameters (2-50 µm) using single nozzle. This change in diameter digitally controlled by combining the mass flow rate nozzle collector speed variations without changing applied voltage. greatest was achieved simultaneous...
In living tissues, cells express their functions following complex signals from surrounding microenvironment. Capturing both hierarchical architectures at the micro- and macroscale, anisotropic cell patterning remains a major challenge in bioprinting, bottleneck toward creating physiologically-relevant models. Addressing this limitation, novel technique is introduced, termed Embedded Extrusion-Volumetric Printing (EmVP), converging extrusion-bioprinting layer-less, ultra-fast volumetric...
Abstract Photopolymerizing hydrogel systems provide a method to encapsulate cells and implant materials in minimally invasive manner. Controlled release of growth factors the hydrogels may enhance ability engineer tissues. IGF‐I TGF‐β were loaded PLGA microspheres using double emulsion technique. 125 ng 200 pg active TGF‐β, respectively, as measured by ELISA, released over 15 days. The factor containing photoencapsulated with bovine articular chondrocytes PEO‐based incubated vitro for two...
Nutrient and oxygen supply of cells are crucial to tissue engineering in general. If a sufficient cannot be maintained, the development will slow down or even fail completely. Previous studies on have focused measurement partial pressures (pO(2)) culture media described use invasive techniques with spatially limited resolution. The experimental setup here allows for continuous, noninvasive, high-resolution pO(2) measurements over cross-section cultivated tissues. Applying recently developed...
Plasma protein adsorption patterns on colloidal drug carriers acquired after iv administration depend their surface characteristics and are regarded as key factors for in vivo organ distribution. Polymeric latex particles with strongly differing properties were synthesized models tissue-specific targeting via the intravenous route. Physicochemical characterization was performed size, charge density, zeta potential, hydrophobicity. The interactions human plasma proteins studied by way of...
The effects of four regulatory factors on tissue-engineered cartilage were examined with specific focus the ability to increase construct growth rate and concentrations glycosaminoglycans (GAG) collagen, major extracellular matrix (ECM) components. Bovine calf articular chondrocytes seeded onto biodegradable polyglycolic acid (PGA) scaffolds cultured in medium or without supplemental insulin-like factor (IGF-I), interleukin-4 (IL-4), transforming factor-β1 (TGF-β1) platelet-derived (PDGF)....
This study investigates the use of allyl-functionalized poly(glycidol)s (P(AGE-co-G)) as a cytocompatible cross-linker for thiol-functionalized hyaluronic acid (HA-SH) and optimization this hybrid hydrogel bioink 3D bioprinting. The chemical cross-linking gels with 10 wt.% overall polymer concentration was achieved by UV-induced radical thiol-ene coupling between thiol allyl groups. addition unmodified high molecular weight HA (1.36 MDa) enabled rheology to be tuned extrusion-based...
In 3D bioprinting, bioinks with high concentrations of polymeric materials are frequently used to enable fabrication cell-hydrogel constructs sufficient stability. However, this is often associated restricted cell bioactivity and an inhomogeneous distribution newly produced extracellular matrix (ECM). Therefore, study investigates bioink compositions based on hyaluronic acid (HA), attractive material for cartilage regeneration, which allow reduction polymer content. Thiolated HA...
Biofabrication, including printing technologies, has emerged as a powerful approach to the design of disease models, such in cancer research. In breast cancer, adipose tissue been acknowledged an important part tumor microenvironment favoring progression. Therefore, this study, 3D-printed model for facilitating investigations into cell-adipocyte interaction was developed. First, we focused on printability human adipose-derived stromal cell (ASC) spheroids extrusion-based bioprinting setup...
Mechanical forces regulate the structure and function of many tissues in vivo; recent results indicate that mechanical environment can decisively influence development engineered cultured vitro. To investigate effects hydrodynamic on tissue-engineered cartilage, primary bovine calf chondrocytes were seeded fibrous polyglycolic acid meshes spinner flasks either statically or at one nine different turbulent mixing intensities. In medium from unmixed flasks, CO2 accumulated O2 was depleted,...
Linear polyethylenimine (LPEI) with a molecular weight (MW) of 22 kDa has been described as having superior ability to induce gene transfer compared its branched form. However, the transfection efficiency polymer cannot be enhanced beyond certain limit due cytotoxicity. We explored potential utilizing LPEIs MWs ranging from 1.0 9.5 overcome this limitation.Polyplexes plasmid DNA encoding for green fluorescent protein (EGFP) and various were concerning their cytotoxicity in CHO-K1 HeLa cells...
The use of either a hydrogel or solid polymeric scaffold alone is often associated with distinct drawbacks in many tissue engineering applications. Therefore, this study, we investigated the potential combination long-term stable fibrin gels and polyurethane scaffolds for cartilage engineering. Primary bovine chondrocytes were suspended gel subsequently injected into polycaprolactone-based scaffold. Cells homogeneously distributed within composite system produced high amounts...
Despite the clinical need for reconstructive and plastic surgery, supply of engineered adipose tissue equivalents still remains a challenge. As yet, only preadipocytes have been applied as cell material in vitro engineering fat. Herein, we report establishment three-dimensional (3-D) long-term culture, using bone marrow-derived mesenchymal stem cells (MSCs) an alternative source custom-made poly(lactic-co-glycolic acid) (PLGA) scaffolds carrier. Cell-polymer constructs were cultivated 4...