A5005822241- RNA Interference and Gene Delivery
- Hydrogels: synthesis, properties, applications
- Protein purification and stability
- Nanoparticle-Based Drug Delivery
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- Glycosylation and Glycoproteins Research
- Advanced Drug Delivery Systems
- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Biosensors and Analytical Detection
- Electrospun Nanofibers in Biomedical Applications
- Virus-based gene therapy research
- Neurobiology and Insect Physiology Research
- Ion Channels and Receptors
- Analytical Chemistry and Chromatography
- Inhalation and Respiratory Drug Delivery
- Advancements in Transdermal Drug Delivery
- Chemical Synthesis and Analysis
- Supramolecular Self-Assembly in Materials
- Biochemical Analysis and Sensing Techniques
- Click Chemistry and Applications
- Minerals Flotation and Separation Techniques
- Microencapsulation and Drying Processes
Roche (Switzerland)
2023
University of Regensburg
2015-2019
Ludwig-Maximilians-Universität München
2013-2016
Howard Hughes Medical Institute
2016
Boston Children's Hospital
2016
Significance Transient receptor potential cation (TRPC) 4 and 5 channels are nonselective activated via G protein-coupled receptors. In contrast to all other TRPC channels, they regarded as insensitive the phospholipase C (PLC) product diacylglycerol (DAG). Deeper insight into protein-dependent activation mechanism of TRPC4/5 is lacking. this study we unravel q/11 protein-mediated signaling pathway leading activation. Depletion phosphatidylinositol 4,5-bisphosphate causes a conformational...
Although there are a large number of studies available for the evaluation therapeutic efficacy targeted polymeric nanoparticles, little is known about critical attributes that can further influence their uptake into target cells. In this study, varying cRGD ligand densities (0-100% surface functionalization) were combined with different poly(ethylene glycol) (PEG) spacer lengths (2/3.5/5 kDa), and specific receptor binding core-shell structured poly(lactic- co-glycolic acid)/poly(lactic...
Formation of a protein corona can severely compromise the performance functionalized polymeric nanoparticles by changing their identity and integrity.
Amine-modified four- and eight-armed poloxamines were prepared subsequently functionalized with maleimide or furyl groups. Aqueous solutions of these polymers exhibited an immediate gelation at a temperature above 37 °C. Concomitantly, Diels–Alder reactions gradually cross-linked cured the gels. Different ratios between macromonomers used to tune hydrogel stability mechanical properties. In this way, could be precisely controlled in range 14 329 days. Controlled release model antibody...
The space of advanced therapeutic modalities is currently evolving in rapid pace necessitating continuous improvement analytical quality control methods. In order to evaluate the identity nucleic acid species gene therapy products, we propose a capillary electrophoresis-based gel free hybridization assay which fluorescently labeled peptide acids (PNAs) are applied as affinity probes. PNAs engineered organic polymers that share base pairing properties with DNA and RNA but have an uncharged...
Biodegradable hydrogels were prepared from furan- and maleimide-functionalized eight-armed poly(ethylene glycol) with an average molecular mass of 40 000 Da (8armPEG40k-furan 8armPEG40k-maleimide) using the Diels-Alder (DA) reaction as a cross-linking mechanism. Hydrophobic 6-aminohexanoic acid (C6) 12-aminododecanoic (C12) spacers introduced between polymer backbone functional end-groups; influence on gel properties was studied. Modification C6 C12 induced hydrophobic interactions...
Organic nanoparticles offer the advantage of high biocompatibility for biomedical applications but suffer frequently from poor visibility in biological environments. While fluorescent-labeling is convenient and allows fast extensive histological analysis, fluorescence imaging quantitative analysis are limited by low resolution significantly hindered tissue autofluorescence. Labeling polymeric with an additional gold tag would allow via transmission electron microscopy (TEM) quantification...
Event Abstract Back to Dual gelation enables delayed antibody release from Diels-Alder hydrogels Manuel Gregoritza1, Viktoria Messmann1, Achim M. Goepferich1 and Ferdinand P. Brandl1* 1 University of Regensburg, Department Pharmaceutical Technology, Germany Introduction: (DA) cross-linked poly(ethylene glycol) (PEG) have the potential become a promising delivery system for therapeutic proteins. The aim this study was combine chemical cross-linking via DA “click chemistry” with physical...