A5059139171- Advanced biosensing and bioanalysis techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- Nanocluster Synthesis and Applications
- Biosensors and Analytical Detection
- Lymphoma Diagnosis and Treatment
- Breast Cancer Treatment Studies
- DNA and Nucleic Acid Chemistry
- CNS Lymphoma Diagnosis and Treatment
- Gene expression and cancer classification
- RNA Interference and Gene Delivery
- Advanced Biosensing Techniques and Applications
- HER2/EGFR in Cancer Research
- Advanced Nanomaterials in Catalysis
- Advanced Breast Cancer Therapies
- Crystallization and Solubility Studies
- Cancer Genomics and Diagnostics
- X-ray Diffraction in Crystallography
- Estrogen and related hormone effects
- Molecular Biology Techniques and Applications
- Lung Cancer Treatments and Mutations
- Molecular Junctions and Nanostructures
- Cancer Immunotherapy and Biomarkers
- Circular RNAs in diseases
- Synthesis and characterization of novel inorganic/organometallic compounds
- Chronic Lymphocytic Leukemia Research
Nanostring Technologies (United States)
2011-2021
Veracyte (United States)
2021
Seattle University
2015
Queen Mary University of London
2013
Royal Marsden Hospital
2011-2013
Vancouver Coastal Health
2012
Medical University of Vienna
2012
Vancouver General Hospital
2012
Breakthrough
2011
Wolfson Foundation
2011
A highly selective, colorimetric polynucleotide detection method based on mercaptoalkyloligonucleotide-modified gold nanoparticle probes is reported. Introduction of a single-stranded target oligonucleotide (30 bases) into solution containing the appropriate resulted in formation polymeric network nanoparticles with concomitant red-to-pinkish/purple color change. Hybridization was facilitated by freezing and thawing solutions, denaturation these hybrid materials showed transition...
Selective colorimetric polynucleotide detection based on Au nanoparticle probes which align in a "tail-to-tail" fashion onto target is described. In this new nanoparticle-based system, particles (∼13 nm diameter), are capped with 3'- and 5'-(alkanethiol)oligonucleotides, used to complex 24-base target. Hybridization of the results formation an extended polymeric nanoparticle/polynucleotide aggregate, triggers red purple color change solution. The due shift surface plasmon resonance...
A study aimed at understanding the factors that control optical properties of DNA-linked gold nanoparticle aggregates containing oligonucleotide linkers varying length (24−72 base pairs) is described. In this system, ∼15 nm diameter Au particles modified with (alkanethiol)-12 oligomers are hybridized to a series ranging from 24 72 pairs (∼80−240 Å) in length. Aggregated room temperature, various macroscopic assemblies have plasmon frequency changes inversely dependent on linker Upon...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTDNA-Directed Synthesis of Binary Nanoparticle Network MaterialsRobert C. Mucic, James J. Storhoff, Chad A. Mirkin, and Robert L. LetsingerView Author Information Department Chemistry, Northwestern University, Evanston, Illinois 60208 Cite this: Am. Chem. Soc. 1998, 120, 48, 12674–12675Publication Date (Web):November 20, 1998Publication History Received31 July 1998Published online20 November inissue 1 December...
Purpose Risk of distant recurrence (DR) among women with estrogen receptor (ER) –positive early breast cancer is the major determinant recommendations for or against chemotherapy. It frequently estimated using Oncotype DX score (RS). The PAM50 risk (ROR) provides an alternative approach, which also identifies intrinsic subtypes. Patients and Methods mRNA from 1,017 patients ER-positive primary treated anastrozole tamoxifen in ATAC trial was assessed ROR NanoString nCounter. Likelihood ratio...
The four intrinsic subtypes of breast cancer, defined by differential expression 50 genes (PAM50), have been shown to be predictive risk recurrence and benefit hormonal therapy chemotherapy. Here we describe the development Prosigna™, a PAM50-based subtype classifier model on NanoString nCounter Dx Analysis System intended for decentralized testing in clinical laboratories. 514 formalin-fixed, paraffin-embedded (FFPE) cancer patient samples were used train prototypical centroids each...
The binding affinity of deoxynucleosides (dA, dG, dC, and dT) to gold nanoparticles was studied using a colorimetric assay determine if nucleobase sequence may play role in alkanethiol-capped oligonucleotides nanoparticles. These data indicate that the deoxynucleoside dT has much lower nanoparticle surface than dA. can be correlated with previous study our lab which indicated containing (dT)20 spacers have significantly higher coverage (dA)20 spacers. To probe physical consequences this...
NanoString's Prosigna™ Breast Cancer Prognostic Gene Signature Assay is based on the PAM50 gene expression signature. The test outputs a risk of recurrence (ROR) score, category, and intrinsic subtype (Luminal A/B, HER2-enriched, Basal-like). studies described here were designed to validate analytical performance nCounter Analysis System across multiple laboratories.Analytical precision was measured by testing five breast tumor RNA samples 3 sites. Reproducibility replicate tissue sections...
Nanotechnology-based techniques are being widely evaluated in medical testing and could provide a new generation of diagnostic assays due to their high degrees sensitivity, specificity, multiplexing capabilities, ability operate without enzymes. In this article, we have modified nanoparticle-based biobarcode amplification (BCA) assay for early sensitive detection HIV-1 capsid (p24) antigen by using antip24 antibody-coated microplates capture viral streptavidin-coated DNAs signal...
Primary central nervous system lymphomas (PCNSL) are aggressive non-Hodgkin affecting the (CNS). Although immunophenotyping studies suggested an uniform activated B-cell (ABC) origin, more recently a spectrum of ABC and germinal center (GC) cases has been proposed, with molecular subtypes PCNSL still being matter debate. With emergence novel therapies demonstrating different efficacy between GC patient groups, precise assignment subtype is becoming indispensable. To determine 77 17 secondary...
For more than a decade there has been increasing interest in the use of nanotechnology and microarray platforms for diagnostic applications. In this report, we describe rapid simple gold nanoparticle (NP)-based genomic assay specific identification avian influenza virus H5N1 its discrimination from other major A strains (H1N1, H3N2). Capture intermediate oligonucleotides were designed based on consensus sequences matrix (M) gene H1N1, H3N2 viruses, hemaglutinin (HA) neuraminidase (NA) genes...
3034 Background: Pembrolizumab is a humanized anti-PD1 antibody that FDA approved for use in patients with advanced melanoma and selected metastatic non-small-cell lung cancer. It has also shown clinical activity number of other tumor types trials, but there need precise accurate test can identify most likely to benefit from therapy. Several immune-related gene expression (Gx) signatures formalin fixed, paraffin embedded (FFPE) tissue were previously reported enrich responders pembrolizumab...
Abstract Aim: To assess the performance of ROR score in predicting DR for postmenopausal patients with ER+ EBC treated tamoxifen or followed by anastrozole when PAM50 test is performed a routine hospital pathology laboratory. Background: The multi-analyte gene-expression tests (Oncotype DX, MammaPrint) that are currently used clinical practice to prognosis after endocrine therapy primary breast cancer central referral 50-gene development designed be local laboratories and has been optimized...
8536 Background: Diffuse large B-cell lymphoma (DLBCL) is an aggressive non-Hodgkin's with two distinct molecular cell-of-origin (COO) subtypes known as germinal center (GCB) or activated (ABC). DLBCL have been reported to be prognostic and potentially predictive of treatment benefit, underscoring the need for a precise accurate MDx test. NanoString's LST based on Lymph2Cx gene expression profile (GEP). These studies describe development analytical robustness clinical accuracy Linear...