Background: Authorities advocate that resistance and aerobic exercise are essential for reducing risk factors chronic disease disability in older adults.However, the incremental effects of combined compared with either modality alone on is generally unknown.Methods: Participants were 136 sedentary, abdominally obese men women recruited from September 30, 2002, through November 15, 2006, at Queen's University.Participants randomized to 1 following 4 groups 6 months: exercise, (combined...

OBJECTIVE—The primary objective was to examine whether the combination of diet and aerobic exercise (DA) or resistance (DR) is associated with greater improvements in metabolic risk factors by comparison only (DO) obese women. A second considered reductions are related concurrent changes abdominal and/or intermuscular fat distribution. RESEARCH DESIGN AND METHODS—A total 38 premenopausal women were randomly assigned one three 16-week treatments: DO (n=13), DA (n=11), DR (n=14). Plasma...

OBJECTIVE: This study had two objectives. First, we examined whether the influence of diet combined with either aerobic (DA) (n = 10) or resistance (DR) exercise has effects on insulin and glucose levels that are different in obese men. Second, tried to determine combination is associated improvements greater than those alone (DO) 9). RESEARCH DESIGN AND METHODS: Insulin were measured after an overnight fast a 75-g oral challenge (OGTT). Visceral adipose tissue (AT), subcutaneous AT,...

The purpose of this investigation was to examine the effect caffeine (an adenosine receptor antagonist) on whole-body insulin-mediated glucose disposal in resting humans. We hypothesized that would be lower after administration compared with placebo. Healthy, lean, sedentary (n = 9) men underwent two trial sessions, one (5 mg/kg body wt) and placebo (dextrose) a double-blind randomized design. Glucose assessed using hyperinsulinemic-euglycemic clamp. Before clamp, there were no differences...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFiber-Optic DNA Sensor for Fluorometric Nucleic Acid DeterminationPaul A. E. Piunno, Ulrich J. Krull, Robert H. Hudson, Masad Damha, and Huguette. CohenCite this: Anal. Chem. 1995, 67, 15, 2635–2643Publication Date (Print):August 1, 1995Publication History Published online1 May 2002Published inissue 1 August 1995https://pubs.acs.org/doi/10.1021/ac00111a022https://doi.org/10.1021/ac00111a022research-articleACS PublicationsRequest reuse...

ADVERTISEMENT RETURN TO ISSUEPREVLetterNEXTA Remarkably Simple Protocol for the N-Formylation of Amino Acid Esters and Primary AminesMojmír Suchý, Adam A. H. Elmehriki, Robert E. HudsonView Author Information Department Chemistry, The University Western Ontario, London, Ontario N6A 5B7, Canada[email protected]Cite this: Org. Lett. 2011, 13, 15, 3952–3955Publication Date (Web):June 28, 2011Publication History Received31 May 2011Published online28 June inissue 5 August...

A boehmite@tryptophan‐Pd nanoparticulate catalyst was prepared by a simple, fast and convenient route. The nanomaterial characterized using various techniques employed as thermally stable for Heck, Stille Suzuki cross‐coupling reactions. Optimized conditions these reactions are described. could be isolated, post‐reaction, simple filtration recycled several consecutive cycles without notable change in its activity.

The quest for small molecules that strongly bind to G-quadruplex-DNA (G4), so-called G4 ligands, has invigorated the research field from its very inception. Massive efforts have been invested discover or rationally design evaluate their G4-interacting properties in vitro through a series of now widely accepted and routinely implemented assays, use them as innovative chemical biology tools interrogate cellular networks might involve G4s. In sharp contrast, only uncoordinated aimed at...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNucleic acid dendrimers: novel biopolymer structuresRobert H. E. Hudson and Masad J. DamhaCite this: Am. Chem. Soc. 1993, 115, 6, 2119–2124Publication Date (Print):March 1, 1993Publication History Published online1 May 2002Published inissue 1 March 1993https://pubs.acs.org/doi/10.1021/ja00059a004https://doi.org/10.1021/ja00059a004research-articleACS PublicationsRequest reuse permissionsArticle Views560Altmetric-Citations126LEARN ABOUT THESE...

Abstract Signal loss due to magnetization transfer (MT) from the macromolecular protons of biological tissues is an important consideration for in vivo detection paramagnetic chemical exchange saturation (PARACEST) agents. In this study, a four‐pool model presented that based on modified Bloch equations and incorporates terms proton processes occur systems presence MRI‐PARACEST contrast The effect exchangeable shift PARACEST agent concentration are modeled macromolecule‐derived MT....

Anti-miRs are oligonucleotide inhibitors complementary to miRNAs that have been used extensively as tools gain understanding of specific miRNA functions and potential therapeutics. We showed previously peptide nucleic acid (PNA) anti-miRs containing a few attached Lys residues were potent inhibitors. Using miR-122 an example, we report here the PNA sequence amino requirements for efficient targeting show anti-miR activity is enhanced substantially by presence terminal-free thiol group, such...

Modified nucleosides possessing intrinsically fluorescent heterocycles as base surrogates that are capable of canonical basepairing becoming important biomolecular tools. These "base-discriminating fluorophores" (BDFs) find use in hybridization-based mismatch detection, elucidation DNA damage and the study localized structural phenomena exemplified ribozymes nucleic acid/ligand interactions. In this review, an historical perspective will be given along with some more recent highlights...

Tissue temperature is a fundamental physiological parameter that can provide insight into pathological processes. The purpose of this study was to develop and characterize novel paramagnetic chemical exchange saturation transfer (CEST) agent suitable for in vivo mapping at 9.4T. CEST properties the europium (Eu(3+)) complex DOTAM-Glycine (Gly)-Phenylalanine (Phe) ligand were studied vitro 9.4T as function temperature, pH, concentration. magnetization (CEST effect) from bound water bulk pools...

The independent effects of weight loss and exercise on plasma leptin total (AT), subcutaneous (SAT), visceral (VAT) adipose tissue were investigated in 52 obese men. Subjects randomly assigned to four 12-wk protocols: 1) control (C, n = 8), 2) diet-induced (DWL, 14), 3) exercise-induced (EWL, 4) with maintenance (EWS, 16). Plasma was unchanged C (from 7.8 ± 1.3 7.7 1.0 ng/ml). Equivalent (7.5 kg) decreased significantly but similarly from 8.5 4.8 0.6 ng/ml; EWL, 10.1 5.0 Exercise the absence...

Altered tissue temperature and/or pH is a common feature in pathological conditions, where metabolic demand exceeds oxygen supply such as tumors and following stroke. Therefore, vivo may become valuable biomarkers for disease detection the monitoring of progression or treatment response conditions with altered demand. In this study, measured using amide protons thulium (Tm 3+ ) complex DOTAM‐Glycine‐Lysine (ligand: Tm ‐DOTAM‐Gly‐Lys). The was uniquely determined from linewidth asymmetry...

Argentaffin tumours are not uncommon and have been known for over half a century-Obern- dorfer called them carcinoid tumoursl in 1907.It is surprising, therefore, that the relationship

A new pyrrolocytosine derivative has been designed to selectively interact with guanine and evaluated in peptide nucleic acid where it imparts increased selective binding affinity for complementary oligonucleotides. The modified nucleobase also possesses an exceptionally high fluorescence quantum yield that is responsive hybridization.

Stabilizing DNA/RNA G-quadruplexes (G4s) using small molecules (ligands) has proven an efficient strategy to decipher G4 biology. Quite paradoxically, this search also highlighted the need for finding able disrupt G4s tackle G4-associated cellular dysfunctions. We report here on both qualitative and quantitative investigations that validate G4-RNA-destabilizing properties of leading compound PhpC in human cells.

Yeast RNA lariat debranching enzyme has been purified to near homogeneity using a bacterial overproducer of the enzyme.The is capable digesting variety branched nucleic acid substrates, including group I1 intron lariats, multicopy single-stranded DNAs (-DNAs), and synthetic RNAs.A trinucleotide release assay radiolabeled msDNA substrates was developed used determine basic biochemical parameters-for shows strong preference for purines at 2'-position in both accord with structure its native...

6-Phenylpyrrolocytidine (PhpC), a structurally conservative and highly fluorescent cytidine analog, was incorporated into oligoribonucleotides. The PhpC-containing RNA formed native-like duplex structures with complementary DNA or RNA. PhpC-modification found to act as sensitive reporter group being non-disruptive structure the enzymatic activity of RNase H. A RNA/DNA hybrid possessing single PhpC insert an excellent substrate for HIV-1 RT Ribonuclease H rapidly reported cleavage strand...