A5088278115- Genetics, Aging, and Longevity in Model Organisms
- Mitochondrial Function and Pathology
- Tryptophan and brain disorders
- Photoreceptor and optogenetics research
- Pancreatic function and diabetes
- ATP Synthase and ATPases Research
- Neurological diseases and metabolism
- Alzheimer's disease research and treatments
- Fungal and yeast genetics research
- Metabolism, Diabetes, and Cancer
- Adenosine and Purinergic Signaling
- Circadian rhythm and melatonin
- Neurobiology and Insect Physiology Research
- Light effects on plants
University of Rochester Medical Center
2022-2024
University of Rochester
2022
Czech Academy of Sciences
2020
Czech Academy of Sciences, Institute of Physiology
2020
Abstract C. elegans react to metabolic distress caused by mismatches in oxygen and energy status via distinct behavioral responses. At the molecular level, these responses are coordinated under-characterized, redox-sensitive processes, thought initiate mitochondria. Complex I of electron transport chain is a major site reactive species (ROS) production canonically associated with oxidative damage following hypoxic exposure. Here, we use combination optogenetics CRISPR/Cas9-mediated genome...
Abstract Organisms rely on chemical cues in their environment to indicate the presence or absence of food, reproductive partners, predators, other harmful stimuli. In nematode Caenorhabditis elegans, bilaterally symmetric pair ASH sensory neurons serves as primary nociceptors. activation by aversive stimuli leads backward locomotion and stimulus avoidance. We previously reported a role for guanylyl cyclases dampening nociceptive sensitivity that requires an innexin-based gap junction network...
Abstract Despite longstanding scientific interest in the centrality of mitochondria to biological aging, directly controlling mitochondrial function test causality has eluded researchers. We show that specifically boosting membrane potential through a light-activated proton pump reversed age-associated phenotypes and extended C. elegans lifespan. harnessing energy light experimentally increase during adulthood alone is sufficient slow rate aging.
Transcript levels for selected ATP synthase membrane FO-subunits-including DAPIT-in INS-1E cells were found to be sensitive lowering glucose down from 11 mM, in which these are routinely cultured. Depending on conditions, the diminished mRNA recovered when was restored mM; or elevated during further 120 min incubations with 20-mM glucose. Asking whether DAPIT expression may by hyperglycemia vivo, we studied mice hyaluronic acid implants delivering up 14 days. Such continuous two-week...
Organisms surveil and respond to their environment using behaviors entrained by metabolic cues that reflect food availability. Mitochondria act as hubs at the center of mitochondrial energy production is protonmotive force (PMF), an electrochemical gradient generated metabolite consumption. The PMF serves a central integrator status, but its role in governing signaling poorly understood. We used optogenetics dissipate Caenorhabditis elegans tissues test food-related behaviors. Our data...