A5010803502- Influenza Virus Research Studies
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Hepatitis Viruses Studies and Epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Hepatitis B Virus Studies
- Respiratory viral infections research
- Complement system in diseases
- Immune Response and Inflammation
- Neuroinflammation and Neurodegeneration Mechanisms
- Food Quality and Safety Studies
- vaccines and immunoinformatics approaches
- Animal Disease Management and Epidemiology
- Diabetes and associated disorders
- Single-cell and spatial transcriptomics
- Herpesvirus Infections and Treatments
- COVID-19 epidemiological studies
- Galectins and Cancer Biology
- Viral Infections and Immunology Research
- Blood groups and transfusion
Boston Children's Museum
2024
Boston Children's Hospital
2023
Harvard University
2023
University of Rochester Medical Center
2018-2022
National Centre for Cell Science
2017
The pandemic influenza A(H1N1) 2009 virus caused significant morbidity and mortality worldwide thus necessitating the need to understand host factors that influence its control. Previously, complement system has been shown provide protection during seasonal infection, however, role of individual pathways is not yet clear. Here, we have dissected intact as well activation infection using mouse strains deficient in various components. We show C3-/- mice results increased viral load 100%...
Avian influenza vaccines exhibit poor immunogenicity in humans. We hypothesized that one factor underlying weak B cell responses was sequence divergence between avian and seasonal hemagglutinin proteins, thus limiting the availability of adequate CD4 T help. To test this, a novel chimeric protein (cH7/3) derived, comprised stem domain from H3 head H7. Immunological memory to established mice, through strategies included inactivated vaccines, Flumist, synthetic peptides derived stalk domain....
Vaccination is widely used to generate protective immunity against influenza virus. CD4+ T cells contribute in diverse ways immunity, most notably, the provision of help for production neutralizing antibodies. Several recent reports have suggested that virus infection elicits whose specificity only partially overlaps elicited by vaccination. This finding has raised serious concerns regarding utility currently licensed inactivated vaccines and novel protein-based vaccines. Here, using...
One of the major contributions to protective immunity influenza viruses that is provided by virus-specific CD4 T cells delivery effector function infected lung. However, there little known about selection and breadth viral epitope-specific home lung after their initial priming. In this study, using a mouse model A infection an unbiased method epitope identification, elicited were identified quantified. We found very diverse specificity primed infection, including epitopes from hemagglutinin,...
Pulmonary CD4 T cells are critical in respiratory virus control, both by delivering direct effector function and through coordinating responses of other immune cells. Recent studies have shown that following influenza infection, virus-specific partitioned between pulmonary vasculature lung tissue. However, very little is known about the peptide specificity or functional differences within these two compartments. Using a mouse model infection conjunction with intravascular labeling vivo, cell...
The adaptive T cell response to influenza B virus is understudied, relative A virus, for which there has been considerable attention and progress many decades. Here, we have developed utilized the C57BL/6 mouse model of intranasal infection with (B/Brisbane/60/2008) and, using an iterative peptide discovery strategy, identified a series robustly elicited individual CD4 specificities. repertoire encompassed at least eleven major epitopes distributed across hemagglutinin, nucleoprotein,...
Summary Mounting evidence implicated the classical complement pathway (CP) in normal brain development, and pathogenesis of neuropsychiatric neurodegenerative diseases. However source regulation remain unclear. Using MERFISH, a spatial transcriptomic method with single-cell resolution, we established developmental atlas system. We showed that synthesizes essential building blocks system locally remarkable cellular heterogeneity. provided transcriptional supporting presence alternative (AP),...
Abstract Presence of spontaneous germinal center (GCs) in SLE patients correlates with the production pathogenic, isotype-switched antibodies (Abs) and epitope spreading. In lupus mice, GCs are a major site for self-reactive B cells to undergo clonal selection self-antigens. Limited efficacy current cell-based therapies highlights need explore alternative targets inhibiting auto-Ab secreting (ASCs) generation. We propose that complement receptor 2 (CD21) is novel therapeutic target block...