A5019489765- Influenza Virus Research Studies
- Herpesvirus Infections and Treatments
- Complement system in diseases
- Animal Disease Management and Epidemiology
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Cytomegalovirus and herpesvirus research
- Viral Infections and Vectors
- Poxvirus research and outbreaks
- Viral gastroenteritis research and epidemiology
- Plant Virus Research Studies
- Animal Virus Infections Studies
- Mosquito-borne diseases and control
- Virology and Viral Diseases
- Immunotherapy and Immune Responses
- Transgenic Plants and Applications
- Immune Response and Inflammation
- Hepatitis B Virus Studies
- Virus-based gene therapy research
- Viral-associated cancers and disorders
- Hepatitis Viruses Studies and Epidemiology
- Trypanosoma species research and implications
- interferon and immune responses
- Immune Cell Function and Interaction
- Bacillus and Francisella bacterial research
ICMR-National Institute of Virology
2012-2025
Savitribai Phule Pune University
2003-2011
National Centre for Cell Science
2003-2007
The Influenza A pandemic H1N1 2009 (H1N1pdm) virus appeared in India May and thereafter outbreaks with considerable morbidity mortality have been reported from many parts of the country. Continuous monitoring genetic makeup is essential to understand its evolution within country relation global diversification track mutations that may affect behavior virus.H1N1pdm viruses were isolated both recovered fatal cases representing major cities sequenced. Phylogenetic analyses six concatenated...
Widespread infection of highly pathogenic avian influenza A H5N1 was reported from backyard and commercial poultry in West Bengal (WB), an eastern state India early 2008. Infection gradually spread to Tripura, Assam Sikkim, the northeastern states, with 70 outbreaks between January 2008 May 2009. Whole genome sequence analysis three isolates WB, one isolate Tripura along hemagglutinin (HA) neuraminidase (NA) genes 17 other performed during this study. In HA gene phylogenetic tree, all...
More than 70 outbreaks of the highly pathogenic avian influenza (HPAI) H5N1 have been reported in poultry western and north-eastern parts India. Therefore, view recent HPAI poultry, active AI surveillance encompassing wild, resident, migratory birds was undertaken during 2009-2011 State West Bengal.A total 5722 samples were collected from Bengal; 3522 (2906 fecal droppings + 616 other environmental samples) 2200 [1604 tracheal, cloacal swabs, samples, tissue 596 blood (serum)] domestic ducks...
The pandemic influenza A(H1N1) 2009 virus caused significant morbidity and mortality worldwide thus necessitating the need to understand host factors that influence its control. Previously, complement system has been shown provide protection during seasonal infection, however, role of individual pathways is not yet clear. Here, we have dissected intact as well activation infection using mouse strains deficient in various components. We show C3-/- mice results increased viral load 100%...
The genome analysis of Kaposi's sarcoma-associated herpesvirus (KSHV) has revealed the presence an open reading frame (ORF 4) with sequence homology to complement control proteins. To assign a function this protein, we have now expressed ORF using Pichia expression system and shown that purified protein inhibited human complement-mediated lysis erythrocytes, blocked cell surface deposition C3b (the proteolytically activated form C3), served as cofactor for factor I-mediated inactivation...
Variola virus, the causative agent of smallpox, encodes a soluble complement regulator named SPICE. Previously, SPICE has been shown to be much more potent in inactivating human than vaccinia virus control protein (VCP), although they differ only 11 amino acid residues. In present study, we have expressed SPICE, VCP, and mutants VCP by substituting each or non-variant residues with corresponding residue identify hot spots that impart functional advantage over VCP. Our data indicate (i) is...
ABSTRACT Vaccinia virus encodes a homolog of the human complement regulators named vaccinia control protein (VCP). It is composed four contiguous (CCP) domains. Previously, VCP has been shown to bind C3b and C4b inactivate classical alternative pathway C3 convertases by accelerating decay convertase (to limited extent) convertase, as well supporting factor I-mediated inactivation (the subunits convertases). In this study, we have mapped CCP domains important for its cofactor activities,...
The vaccinia virus complement control protein (VCP) is an immune evasion of virus. Previously, VCP has been shown to bind and support inactivation host proteins C3b C4b protect the virions from antibody-dependent complement-enhanced neutralization. However, molecular mechanisms involved in interaction with its target have not yet elucidated. We utilized surface plasmon resonance technology study C4b. measured kinetics binding viral compared it human regulators factor H sCR1, assessed...
Recently it has been shown that kaposica, an immune evasion protein of Kaposi's sarcoma-associated herpesvirus, inactivates complement by acting on C3-convertases accelerating their decay as well a cofactor in factor I-mediated inactivation subunits C3b and C4b. Here, we have mapped the functional domains kaposica. We show SCRs 1 2 (SCRs 1-2) 1-4 are essential for classical alternative pathway C3-convertase decay-accelerating activity (DAA), respectively, while 2-3 required I (CFA) SCR 3 4,...
Herpesvirus saimiri (HVS) is a lymphotropic virus that causes T-cell lymphomas in New World primates. It encodes structural homolog of complement control proteins named protein (CCPH). Previously, CCPH has been shown to inhibit C3d deposition on target cells exposed complement. Here we have studied the mechanism by which it inactivates We expressed soluble form Escherichia coli, purified homogeneity and compared its activity vaccinia (VCP) human regulators factor H receptor 1. The bound C3b...
Poxviruses encode a repertoire of immunomodulatory proteins to thwart the host immune system. One among this array is homolog complement regulatory that conserved in various poxviruses including vaccinia (VACV) and variola. The virus control protein (VCP), which inhibits by decaying classical pathway C3-convertase (decay-accelerating activity), supporting inactivation C3b C4b serine protease factor I (cofactor was shown play role viral pathogenesis. However, its individual activities impart...
Significance The complement system discriminates self from nonself targets solely owing to the presence of regulators on host cells, which primarily belong activation (RCA) protein family. These control cell surface by two mechanisms termed “cofactor activity” (CFA) and “decay-accelerating activity.” Here, we have identified critical structural determinants an RCA responsible for imparting CFA show that these bridge MG2 (macroglobulin-2) CUB (complement C1r-C1s, Uegf, Bmp1) domains C3b...
Down-regulation or loss of MHC class I expression is a major mechanism used by cancer cells to evade immunosurveillance and increase their oncogenic potential. mediated antigen presentation complex regulatory process, controlled processing machinery (APM) dictating immune response. Transcriptional regulation the APM that can modulate gene profile correlation in remain enigmatic. Here, we reveal Scaffold/Matrix-Associated Region 1- binding protein (SMAR1), positively regulates surface...