A5002017870- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Vibrio bacteria research studies
- Advanced biosensing and bioanalysis techniques
- Pancreatic function and diabetes
- Viral gastroenteritis research and epidemiology
- Cancer Research and Treatments
- Renal and related cancers
- Herpesvirus Infections and Treatments
- Cancer-related Molecular Pathways
- Influenza Virus Research Studies
- Field-Flow Fractionation Techniques
- PI3K/AKT/mTOR signaling in cancer
- Epigenetics and DNA Methylation
- thermodynamics and calorimetric analyses
- Biosimilars and Bioanalytical Methods
- TGF-β signaling in diseases
- Pancreatic and Hepatic Oncology Research
- Child Nutrition and Water Access
- Pharmaceutical Economics and Policy
- Travel-related health issues
- Viral Infections and Immunology Research
- Escherichia coli research studies
Emergent BioSolutions (United States)
2021-2023
PAX Scientific (United States)
2015-2016
PaxVax (United States)
2015-2016
We have investigated the use of column chromatography for purification ACN53, a recombinant adenovirus type 5 encoding human p53 tumor suppressor protein. Anion exchange, size exclusion, hydrophobic interaction, and metal chelating resins were tested; each was found to distinct advantages disadvantages. Based on these data, rapid method devised ACN53. The resultant product characterized compared cesium chloride density-gradient purified virus by SDS-PAGE, Western blot analysis, absorbance...
The expanding use of adenoviral vectors for gene therapy has brought about the need new analytical tools. We have developed an anion-exchange high-performance liquid chromatography method to analyze recombinant adenovirus serotype 5 samples. Before this assay, available methods consisted either long-term biological assays or required highly purified test articles. These were inadequate optimizing production and purification. This assay can quantitate viral particles in crude lysates pure It...
Background Zika virus (ZIKV), a mosquito-borne flavivirus, is re-emerging that constitutes public health threat due to its recent global spread, recurrent outbreaks, and infections are associated with neurological abnormalities in developing fetuses Guillain-Barré syndrome adults. To date, there no approved vaccines against ZIKV infection. Various preclinical clinical development programs currently ongoing an effort bring forward vaccine for ZIKV. Methodology/Principle findings We have...
C'est magnifique, mais ne c'est pas science. This is the phrase that most comes to mind when multiplicity of infection (m.o.i.) used as a descriptive measurement in scientific presentations. Multiplicity commonly defined ratio infectious virions cells culture. On surface, concept simple, easy calculate, and use speech written reports. Nevertheless, m.o.i. an overly simplistic depiction complex setting. Yes, it gives impression quantitative preciseness. However, would be more precise refer...
Recombinant adenoviral (rAd) vectors are capable of mediating high-efficiency gene transfer in vivo. Under conditions requiring systemic administration, however, the use rAd can be problematic due to presence circulating anti-adenovirus antibodies developed either through natural infection or during course treatment. We a passive immunization model SCID/Beige mice assess effect human and mouse on administration vector expressing beta-galactosidase (rAd-betagal). In this model, vitro...
ABSTRACT Replication-competent adenovirus (rcAd)-based vaccine vectors may theoretically provide immunological advantages over replication-incompetent Ad vectors, but they also raise additional potential clinical and regulatory issues. We produced replication-competent serotype 26 (rcAd26) by adding the E1 region back into a Ad26 vector backbone with E3 or E3/E4 regions deleted. assessed effect of vectorization on replicative capacity rcAd26 vaccines. Attenuation occurred in stepwise...
PXVX0047 is an investigational vaccine developed for active immunization to prevent febrile acute respiratory disease (ARD) caused by adenovirus serotypes 4 (Ad4) and 7 (Ad7). consists of a modernized, plasmid-derived that was generated using virus isolated from Wyeth Ad4 Ad7 tablets. A phase 1 two-arm, randomized, double-blind, active-controlled study conducted evaluate the safety profile immunogenicity vaccines. The two components were administered orally together in single dose 11...
The FDA-approved Adenovirus Type 4 and 7 Vaccine, Live, Oral is highly effective essential in preventing acute respiratory diseases (ARDs) U.S. military recruits. Our study revealed the presence of a previously undetected mutation, not found wild-type human adenovirus type (HAdV-4) component licensed vaccine, which contains an amino acid substitution (P388T) pre-terminal protein (pTP). This demonstrated that replication T388 HAdV-4 vaccine mutant virus favored over wild WI-38 cells, cell...
<h3>Abstract</h3> Water balance, tracked by extracellular osmolality, is regulated feedback and feedforward mechanisms. Feedback regulation reactive, occurring as deviations in osmolality are <i>detected</i>. Feedforward or presystemic proactive, when disturbances <i>anticipated</i>. Vasopressin (AVP) a key hormone regulating water balance released during hyperosmolality to limit renal excretion. AVP neurons under regulation. Not only do they respond blood but also rapidly suppressed...
PXVX0200 is an oral cholera vaccine that approved for use by the U.S. Food and Drug Administration European Medicines Agency under tradename Vaxchora. The supplied as two packets, one containing buffer component other active component, are mixed with water ingested. aim of this study was to develop preparation methods appropriate administering children. Developing liquid medication children has unique challenges, including administration volume palatability. These challenges were addressed...
Abstract CVD 103-HgR live, attenuated oral cholera vaccine strain is indicated for single dose immunization against Vibrio cholerae , the causative agent cholera. The packets containing buffer powder and lyophilized are reconstituted in water consumed. Studies were performed to explore viability of drinking waters from common sources. was bottled tap United States Europe, measured via colony forming units assay. Chemical analysis select samples used identify chemicals that have a negative...