A5070570645- Antibiotic Resistance in Bacteria
- Viral Infections and Vectors
- Gut microbiota and health
- Viral gastroenteritis research and epidemiology
- Mosquito-borne diseases and control
- Virus-based gene therapy research
- Soil Carbon and Nitrogen Dynamics
- Microbial Community Ecology and Physiology
- Bacterial biofilms and quorum sensing
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacteriophages and microbial interactions
- Vector-Borne Animal Diseases
- Antibiotic Use and Resistance
- Plant Molecular Biology Research
- Vector-borne infectious diseases
- Viral Infections and Immunology Research
- Insect symbiosis and bacterial influences
- Genomics and Phylogenetic Studies
- Respiratory viral infections research
- Tuberculosis Research and Epidemiology
- Bacterial Genetics and Biotechnology
- Plant tissue culture and regeneration
- Legume Nitrogen Fixing Symbiosis
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
Walter Reed Army Institute of Research
2015-2025
Huazhong Agricultural University
2025
Northwest A&F University
2022-2024
Ministry of Agriculture and Rural Affairs
2022-2024
Zhejiang Chinese Medical University
2024
Southwest Medical University
2023-2024
Sichuan University
2024
Zhejiang University
2008-2023
Beijing Forestry University
2023
Guizhou University
2009-2023
With the aim of gathering temporal trends on bacterial epidemiology and resistance from multiple laboratories in China, CHINET surveillance system was organized 2005. Antimicrobial susceptibility testing carried out according to a unified protocol using Kirby-Bauer method or automated systems. Results were analyzed Clinical Laboratory Standards Institute (CLSI) 2014 definitions. Between 2005 2014, number isolates ranged between 22 774 84 572 annually. Rates extended-spectrum β-lactamase...
ObjectivesTo characterize the prevalence of plasmid-mediated quinolone resistance determinants qnr and aac(6′)-Ib-cr in extended-spectrum β-lactamase (ESBL)-producing Escherichia coli Klebsiella pneumoniae.
Significance Pluripotent stem cells are critical in both animal and plant development. Unlike higher animals, plants have unique postembryonic development, which continuously produce organs depending on shoot root meristems. In the meristem, division of outermost layers provides for all structures. How those meristem tightly controlled attracted intense studies. Genetic molecular analyses Arabidopsis identified key transcription factors WUSCHEL (WUS) SHOOTMERISTEMLESS (STM) as major...
ABSTRACT In response to global concerns over the spread of New Delhi metallo-β-lactamase gene 1, bla NDM-1 , a monthly surveillance program was initiated in September 2010. All carbapenem-resistant Gram-negative strains forwarded our facility are screened for this gene. To date, 321 isolates, encompassing 11 bacterial species, have been tested. February 2011, two Providencia stuartii submitted from military hospital Afghanistan, tested positive . Both were identical by pulsed-field gel...
Sample storage conditions, extraction methods, PCR primers, and parameters are major factors that affect metagenomics analysis based on microbial 16S rRNA gene sequencing. Most published studies were limited to the comparison of only one or two types these factors. Systematic multi-factor explorations needed evaluate conditions may impact validity a microbiome analysis. This study was aimed improve methodological options facilitate best technical approaches in design study. Three readily...
This study represents a systematic chemical and biological of the rufomycin (RUF) class cyclic heptapeptides, which our anti-TB drug discovery efforts have identified as potentially promising agents that newly target caseinolytic protein C1, ClpC1. Eight new RUF analogues, rufomycins NBZ1–NBZ8 (1–8), well five known peptides (9–13) were isolated characterized from Streptomyces atratus strain MJM3502. Advanced Marfey's X-ray crystallographic analysis led to assignment absolute configuration...
Seed size is a major factor determining crop yields that controlled through the coordinated development of maternal and zygotic tissues. Here, we identified Arabidopsis MATERNAL EFFECT EMBRYO ARREST45 (MEE45) as B3 transcription controls cell proliferation maternally regulates seed its transcriptional activation AINTEGUMENTA (ANT) downstream control auxin biosynthesis in ovule integument. After characterizing reduced organ phenotypes mee45 mutants finding overexpression MEE45 causes...
Over the past decade, continuous and rapid advances in bioinformatics have led to an increasingly common use of molecular sequence comparison for phylogenetic analysis. However, multi-software cross-platform strategies has increased complexity tree estimation. Therefore, development application streamlined analysis tools are growing significance field biology. Particularly genomes with relatively short sequences, there is a lack simple integrative In this study, we present CamlTree...
Bangladesh has a warm climate and landscapes favourable for the proliferation of mosquitoes. Mosquito-borne pathogens including malaria arthropod-borne viruses (arboviruses) remain serious threat to public health requiring constant vector control disease surveillance. From November 2018 April 2019, Anopheles mosquitoes were collected in three unions Ramu Upazila (sub-district) Cox’s Bazar District, Bangladesh. The mosquito specimens combined into pools based on date collection, household ID,...
Here, we present the genome sequences of one mesonivirus and four novel arboviruses observed in Culex bitaeniorhynchus pipiens, captured near demilitarized zone, Republic Korea. Results suggest ubiquitous presence mesoniviruses discovery a potentially new species field-captured mosquitoes.
Recent outbreaks of emerging and re-emerging viruses have shown that timely detection novel arboviruses with epidemic potential is essential to mitigate human health risks. There are rising concerns emergent JEV genotype V (GV) circulating in Asia, against which current vaccines may not be efficacious. To ascertain if GV other East we conducted next-generation sequencing on 260 pools Culex tritaeniorhynchus bitaeniorhynchus mosquitoes (6540 specimens) collected at Camp Humphreys, Republic...
Induced hypoxia stress on cervical cancer derived cells (HeLa cells) leads to significant changes in their membrane lipid profiles.
Arboviruses continue to be a significant global health concern. The unbiased metagenomic analyses of mosquito-borne and mosquito-specific viruses are useful understand viral diversity for the surveillance pathogens medical veterinary importance. Metagenomic analysis was conducted on 6368 mosquitoes (736 pools), covering 16 species from 18 locations throughout Republic Korea (ROK) in 2016. In this report, we describe three detected single pool Aedes vexans nipponii collected at Yongsan U.S....
Introduction In an era increasingly defined by the challenge of antibiotic resistance, this study offers groundbreaking insights into antibacterial properties two distinct Lactiplantibacillus plantarum strains, TE0907 and TE1809, hailing from unique ecosystem Bufo gargarizans . It uniquely focuses on elucidating intricate components mechanisms that empower these strains with their notable capabilities. Methods The research employs a multi-omics approach, including agar diffusion tests to...
Despite major advances in next-generation sequencing, assembly of sequencing data, especially data from novel microorganisms or re-emerging pathogens, remains constrained by the lack suitable reference sequences. De novo is best approach to achieve an accurate finished sequence, but multiple platforms paired-end libraries are often required full genome coverage. In this study, we demonstrated a method assemble complete bacterial sequences integrating shotgun Roche 454 pyrosequencing with...
Abstract Human adenovirus type 55 (HAdV-55) causes acute respiratory disease of variable severity and has become an emergent threat in both civilian military populations. HAdV-55 infection is endemic to China South Korea, but data from other regions time periods are needed for comprehensive assessment prevalence a global perspective. In this study, we subjected isolates various countries collected during 1969–2018 whole-genome sequencing, genomic proteomic comparison, phylogenetic analyses....
Human adenoviruses (AdV) are mostly associated with minimal pathology. However, more severe respiratory tract infections and acute diseases, most often caused by AdV-4 AdV-7, have been reported. The only licensed vaccine in the United States, live oral AdV-7 vaccine, is indicated for use military, nearly exclusively recruit populations. excellent safety profile prominent antibody response of well established placebo-controlled clinical trials, while, long-term immunity vaccination has not...
Abstract Host plants and the microbiota living in rhizosphere are interdependent, mutually reinforced beneficial but assembly, functions microbial interactions of host‐associated still unclear. Herein, winter wheat was selected as a test plant to explore assembly process total bacteria from bulk soil (BS) (RS), phoD ‐harbouring RS at long‐term (14 years) trial site. We also identified core microbes that potentially relevant carbon (C), nitrogen (N) phosphorus (P) cycling genes biogeochemical...
The genus Bartonella contains >40 species, and an increasing number of these species are being implicated in human disease. One such pathogen is ancashensis, which was isolated blood samples from 2 patients living Caraz, Peru, during a clinical trial treatment for bartonellosis. Three B. ancashensis strains were analyzed by using whole-genome restriction mapping high-throughput pyrosequencing. Genome-wide comparative analysis showed that has features seen modern ancient lineages more related...
Numerous pathogens cause respiratory infections with similar symptoms. Routine diagnostics detect only a limited number of pathogens, leaving gap in illness etiology surveillance. This study evaluated next-generation sequencing for unbiased pathogen identification. Respiratory samples collected Thailand, Philippines, Bhutan, and Nepal, that were negative by several molecular immunofluorescence assays, underwent viral cultivation. Samples which demonstrated cytopathic effect culture (N = 121)...
Abstract Human adenoviruses (HAdV) are genetically diverse and can infect a number of tissues with severities varied from mild to fatal. HAdV types 3, 4, 7, 11, 14, 21, 55 were associated acute respiratory illnesses outbreaks in the United States other countries. The risk be effectively controlled by vaccination or mitigated screening preventive measures. During influenza season 2018–2019, DoD Global Respiratory Pathogen Surveillance Program (DoDGRS) received 24 300 specimens. samples that...