A5040764504- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Urinary Tract Infections Management
- CRISPR and Genetic Engineering
- Antibiotic Use and Resistance
- Antibiotics Pharmacokinetics and Efficacy
- Viral gastroenteritis research and epidemiology
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Biosensors and Analytical Detection
- Gut microbiota and health
- Pharmaceutical and Antibiotic Environmental Impacts
- Pneumonia and Respiratory Infections
- Antimicrobial Resistance in Staphylococcus
- Clostridium difficile and Clostridium perfringens research
- Enterobacteriaceae and Cronobacter Research
- Mycobacterium research and diagnosis
- Mosquito-borne diseases and control
- Helicobacter pylori-related gastroenterology studies
- Escherichia coli research studies
- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Data-Driven Disease Surveillance
- COVID-19 epidemiological studies
- Streptococcal Infections and Treatments
Anhui Medical University
2016-2025
First Affiliated Hospital of Anhui Medical University
2016-2025
Anhui Mental Health Center
2023-2025
Fourth Affiliated Hospital of Anhui Medical University
2018-2023
Hefei University
2020
Haikou City People's Hospital
2019
Chinese Center For Disease Control and Prevention
2019
State Key Laboratory of Infectious Disease Prevention and Control
2019
Beijing Hospital
2019
Jinan Central Hospital
2019
With the aim of gathering temporal trends on bacterial epidemiology and resistance from multiple laboratories in China, CHINET surveillance system was organized 2005. Antimicrobial susceptibility testing carried out according to a unified protocol using Kirby-Bauer method or automated systems. Results were analyzed Clinical Laboratory Standards Institute (CLSI) 2014 definitions. Between 2005 2014, number isolates ranged between 22 774 84 572 annually. Rates extended-spectrum β-lactamase...
Adaptation to selective pressures is crucial for clinically important pathogens establish epidemics, but the underlying evolutionary drivers remain poorly understood. The current epidemic of carbapenem-resistant Klebsiella pneumoniae (CRKP) poses a significant threat public health. In this study we analyzed genome sequences 794 CRKP bloodstream isolates collected in 40 hospitals China between 2014 and 2019. We uncovered subclonal replacement predominant clone ST11, where previously prevalent...
Introduction: Carbapenemase-mediated antimicrobial resistance is currently a hot spot of global concern. Carbapenem-resistant organisms are highly prevalent in hospitals associated with difficult-to-treat infections, resulting poor clinical outcome due to limited treatment options. It urgently needed have rapid, efficient, and convenient molecular assay for identifying such resistant strains. Methods: For this end, we developed new laboratory targeting Klebsiella pneumoniae carbapenemase...
In this first national bloodstream infection (BSI) surveillance program in China, we assessed the composition of pathogenic bacteria and trends for antimicrobial susceptibility over a 6-year period China.Blood bacterial isolates from patients at hospitals participating Blood Bacterial Resistant Investigation Collaborative System (BRICS) were collected January 2014 to December 2019. Only isolate species per patient was eligible full study period. Antibiotic-susceptibility testing conducted by...
Abstract Objective The aim of this study was to investigate the association serum carcinoembryonic antigen (CEA), nerve-specific enolase (NSE), cytokeratin 19 fragment (CYFRA21-1), squamous cell carcinoma (SCC-Ag), and pro-gastrin-releasing peptide (ProGRP) with clinicopathological characteristics chemotherapeutic outcomes patients lung cancer. Methods A total 189 cancer (lung group) diagnosed at Fourth Affiliated Hospital Anhui Medical University from January 2020 December 2021 were...
Purpose Clostridioides difficile ( C. ) infection is the most common cause of nosocomial infection, which a severe challenge in modern medical care. Currently, many laboratory diagnostic methods for are available, such as PCR, culture-based tests, and antigen-based tests. However, these not suitable rapid point-of-care testing (POCT). Therefore, it great significance to develop rapid, sensitive, cost-effective method detect toxin genes. Methods Recently, development clustered regularly...
Introduction Zinc exhibits potent antimicrobial properties due to its ability compromise bacterial structure and protein functionality, effectively inhibiting eradicating bacteria. However, bacteria have evolved mechanisms expel excess zinc ions from their cells, enabling them thrive in environments rich metal at high concentrations. This evolutionary advancement limits the clinical application of as agents. In this study, we aimed investigate potential ionophores overcome resistance by...
We investigated the relationship between outer membrane protein OprD2 and carbapenem-resistance in 141 clinical isolates of Pseudomonas aeruginosa collected January December 2013 from First Affiliated Hospital Anhui Medical University China. Agar dilution methods were employed to determine minimum inhibitory concentration meropenem (MEM) imipenem (IMP) for P. aeruginosa. The gene encoding was amplified from141 analyzed by PCR DNA sequencing. Differences effects IMPR IMPS groups on resistance...
To examine the in vitro and vivo antimicrobial activities of ceftazidime/avibactam (CZA) alone or combination with aztreonam (ATM) against KPC-, NDM-, IMP-, KPC+IMP-, KPC+NDM-producing strains.A total 67 clinical non-repetitive carbapenem-resistant Enterobacterales (CRE) strains were selected for microdilution broth method that was performed to analyze minimal inhibitory concentration (MIC) susceptibility test using checkerboard titration method. The fractional (FIC) calculated determine...
More than half of the world's people are infected or have been with Helicobacter pylori. This infection is related to many diseases, its pathogenicity virulence factors. Therefore, rapid diagnosis H. pylori and genotyping genes play an extremely important role in clinical treatment control transmission.To this end, we developed a molecular detection method based on RPA- CRISPR-Cas12a technology for specific 16S rDNA gene, cytotoxin associated gene A(cagA), vacuolating A (vacA) pylori.The...
Clostridioides difficile is one of the leading pathogens causing nosocomial infection. The infection can range from mild to severe, and rapid identification pivotal for early clinical diagnosis appropriate treatment. Here, a genetic testing platform toxins, referred as OC-MAB (orthogonal CRISPR system combined with multiple recombinase polymerase amplification [RPA]), was developed detect C. toxin genes tcdA tcdB. While recognizing amplified products gene tcdB gene, Cas13a Cas12a could...
Colistin has been considered as one of the most effective treatments in clinical infections, especially multidrug-resistant (MDR) bacteria-infected patients. The mcr-1 gene leads to polymyxin resistance China. present study investigated prevalence a Chinese teaching hospital, and molecular phenotypes positive strains were analyzed.A total 1,112 Escherichia coli collected from University Hospital January 2015 2016. was detected by PCR. All specimens subjected susceptibility testing, analysis,...
Background: Fecal microbiota transplantation (FMT) has emerged as a new therapy targeting gastrointestinal for the treatment of growing number diseases in recent years. Previous studies have suggested that FMT may be potential type 2 diabetes (T2D), but underlying mechanism remains unclear. Therefore, present study, we aimed to investigate role T2D and its mechanisms. Methods: To induce T2D, mice were fed high-fat diet injected with low-dose streptozotocin (STZ) four weeks. The then randomly...
Norovirus is highly infectious and rapidly transmissible represents a major pathogen of sporadic cases outbreaks acute gastroenteritis worldwide, causing substantial disease burden. Recent years have witnessed dramatic increase in norovirus China, significantly higher than previous years, among which GII the predominant prevalent strain. Therefore, rapid diagnosis critical for clinical treatment transmission control. Hence, we developed molecular assay based on RPA combined with...
Abstract The recent major worldwide outbreak of monkeypox virus (MPXV) has highlighted the urgent need for accurate MPXV detection methods. Although quantitative PCR (qPCR) technique is currently gold standard diagnosis, high costs associated with and complex instrumentation, limits its application in resource‐poor settings. CRISPR technology developed rapidly years provides an effective tool point‐of‐care testing pathogen identification. Here, we exploited cleavage properties Cas12a enzyme...
Abstract The widespread dissemination of coronavirus 2019 imposes a significant burden on society. Therefore, rapid detection facilitates the reduction transmission risk. In this study, we proposed multiplex diagnostic platform for rapid, ultrasensitive, visual, and simultaneous severe acute respiratory syndrome 2 (SARS‑CoV‑2) open reading frame 1ab (ORF1ab) N genes. A visual method was developed using clustered regularly interspaced short palindromic repeat (CRISPR)‐Cas12a/Cas13a...