A5047942219- Atomic and Subatomic Physics Research
- Advanced MRI Techniques and Applications
- Advanced NMR Techniques and Applications
- Quantum, superfluid, helium dynamics
- MRI in cancer diagnosis
- Medical Imaging Techniques and Applications
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Renal and Vascular Pathologies
- Pediatric Urology and Nephrology Studies
- Prion Diseases and Protein Misfolding
- Nuclear Physics and Applications
- Porphyrin Metabolism and Disorders
- Neuroscience of respiration and sleep
- Biochemical effects in animals
- Advanced Neuroimaging Techniques and Applications
- Respiratory Support and Mechanisms
- Ultrasound and Hyperthermia Applications
- Asthma and respiratory diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Medical Imaging and Pathology Studies
- 3D Printing in Biomedical Research
- Thermal Regulation in Medicine
- Lanthanide and Transition Metal Complexes
- Kidney Stones and Urolithiasis Treatments
- Congenital Diaphragmatic Hernia Studies
Duke University
2012-2020
Duke Medical Center
2012-2019
Duke University Hospital
2014-2017
Assessing functional impairment, therapeutic response and disease progression in patients with idiopathic pulmonary fibrosis (IPF) continues to be challenging. Hyperpolarized
In this study, hyperpolarized 129 Xe MR ventilation and 1 H anatomical images were obtained from three subject groups: young healthy volunteers (HVs), subjects with chronic obstructive pulmonary disease (COPD) age‐matched controls (AMCs). Ventilation quantified by two methods: an expert reader‐based defect score percentage (VDS%) a semi‐automated segmentation‐based (VDP). Reader‐based values assigned experienced radiologists resolved consensus. the analysis, both segmented following...
Hyperpolarized 129 Xe magnetic resonance imaging (MRI) using Dixon-based decomposition enables single-breath of in the airspaces, interstitial barrier tissues, and red blood cells (RBCs). However, methods to quantitatively visualize information from these images pulmonary gas transfer are lacking. Here, we introduce a novel method transform data into quantitative maps ventilation, RBC compartments.A total 13 healthy subjects 12 idiopathic fibrosis (IPF) underwent thoracic 1 H MRI...
Objectives The aim of this study was to investigate ventilation in mild moderate asthmatic patients and age-matched controls using hyperpolarized (HP) 129 Xenon magnetic resonance imaging (MRI) correlate findings with pulmonary function tests (PFTs). Materials Methods This single-center, Health Insurance Portability Accountability Act–compliant prospective approved by our institutional review board. Thirty subjects (10 young patients, 26 ± 6 years; 3 males, 7 females; 10 older 64 healthy...
Although some central aspects of pulmonary function (ventilation and perfusion) are known to be heterogeneous, the distribution diffusive gas exchange remains poorly characterized. A solution is offered by hyperpolarized 129Xe magnetic resonance (MR) imaging, because this can separately detected in lung's air spaces dissolved its tissues. Early dissolved-phase images exhibited intensity gradients that favored dependent lung. To quantitatively corroborate finding, we developed an interleaved,...
A variety of pulmonary pathologies, in particular interstitial lung diseases, are characterized by thickening the blood–gas barrier, and this results reduced gas exchange. Such diffusive impairment is challenging to quantify spatially, because distributions metabolically relevant gases (CO 2 O ) cannot be detected directly within lungs. Hyperpolarized (HP) 129 Xe a promising surrogate for these metabolic gases, MR spectroscopy imaging allow gaseous alveolar separately from dissolved red...
The goal of this work is to characterize and optimize gridding reconstruction 3D radial hyperpolarized (HP) 129 Xe MRI. In support objective, we developed a flexible, open source software package in MATLAB optimally reconstruct radially acquired, undersampled HP Using framework, demonstrate the effects 5 key parameters: overgridding, kernel function, sharpness, extent, density compensation algorithm. We further how each parameter can be tuned high‐resolution acquired image ventilated mouse....
Purpose The purpose of this work was to accurately characterize the spectral properties hyperpolarized 129 Xe in patients with idiopathic pulmonary fibrosis (IPF) compared healthy volunteers. Methods Subjects underwent breath‐hold spectroscopy, during which 38 dissolved‐phase free induction decays (FIDs) were acquired after reaching steady state (echo time/repetition time = 0.875/50 ms; bandwidth 8.06 kHz; flip angle≈22 °). FIDs averaged and then decomposed into multiple components using...
Abstract Hyperpolarized 129 Xe magnetic resonance imaging (MRI) is capable of regional mapping pulmonary gas-exchange and has found application in a wide range disorders humans animal model analogs. This study the first MRI to monocrotaline rat hypertension. Such models preclinical hypertension, disease vasculature that results right heart failure death, are usually assessed with invasive procedures such as catheterization histopathology. The work here adapted from protocols clinical enable...
129 Xe interacts with biological media to exhibit chemical shifts exceeding 200 ppm that report on physiology and pathology. Extracting this functional information requires be measured precisely. Historically, have been reported relative the gas-phase resonance originating from pulmonary airspaces. However, frequency is not fixed-it affected by bulk magnetic susceptibility, as well Xe-N2 , Xe-Xe, Xe-O2 interactions. In study, we addressed introducing a robust method determine 0 reference in...
The objective of our study was to identify the magnitude and distribution ventilation defect scores (VDSs) derived from hyperpolarized (HP)
Chronic kidney disease (CKD) can be characterized by inflammation and fibrosis of the kidney. Although standard care methods have been limited in scope, safety, spatial distribution, MRI diffusion tensor imaging (DTI) has emerged as a promising noninvasive technology to evaluate renal vivo. In this study, we performed DTI an oxalate mouse model CKD systematically identify local injury. parameters strongly correlated with histology, blood biomarkers, hydroxyproline, gene expression.
Voxel-based analysis (VBA) can enable voxel-wise statistical of control and disease images without the need for user-defined regions-of-interest (ROI), but has not been widely used beyond neuroimaging. We demonstrate that VBA be successfully applied to mouse models renal fibrosis. Diffusion MRI parametric maps groups were registered non-linearly a common coordinate space identified distinct regions fibrosis in two different models, which strongly corresponded whole-kidney histology....
Motivation: The TauPS2APP mouse is a well-established model of Alzheimer’s Disease. Traditionally, analyses focus on hippocampus and isocortex, but brain-wide analysis may yield more comprehensive description brain degeneration identify additional regional biomarkers. Goal(s): Identifying robust signatures resulting from amyloidosis tauopathyApproach: We conducted voxel-based longitudinal MRI data mice collected three studies (n=123). Results: found expected persistent hippocampal...
Motivation: MRI is commonly used to monitor different neuropathologies, but has not been widely for ALS research. Goal(s): We sought investigate the utility of longitudinal and MRS assess course disease progression in an inducible TDP-43 mouse model ALSApproach: T2, volume NAA/tCr (N-acetyl aspartate/total creatine) were measured longitudinally disease-relevant regions. Results: These metrics significantly changed 6-week induction phase reversed recovery phase, which was consistent with...
Human genome-wide association studies have identified over 50 loci associated with pulmonary function and related phenotypes, yet follow-up to determine causal genes or variants are rare. Single nucleotide polymorphisms in serotonin receptor 4 (HTR4) human animal work has demonstrated that Htr4 is causally mice, although the precise mechanisms were not identified. We sought elucidate role of neural innervation architecture lung phenotype Htr4-/- animals. report here mouse dependent on vagal...
Three-dimensional (3D) printing has significantly impacted the quality, efficiency, and reproducibility of preclinical magnetic resonance imaging. It vastly expanded ability to produce MR-compatible parts that readily permit customization animal handling, achieve consistent positioning anatomy RF coils promptly, accelerate throughput. permits rapid cost-effective creation customized a specific imaging study, species, weight, or even one unique animal, not routinely used in research. We...
Accurate estimation of kidney function is crucial to the detection, evaluation, and treatment chronic disease (CKD). The traditional methods are inaccurate or invasive. Here, we used DTI-MRI evaluate with progression in nephrotoxic serum- induced CKD model (NTN). We detect changes associated renal inflammation fibrosis prior functional decline. Moreover, AD ADC cortex-outer medulla border were highly correlated histologic evidence fibrosis. These results suggest that can early stages...