A5058961190- Bone Metabolism and Diseases
- Hepatitis C virus research
- Enzyme Structure and Function
- NF-κB Signaling Pathways
- Cytokine Signaling Pathways and Interactions
- Hepatitis B Virus Studies
- Protein Structure and Dynamics
- Photosynthetic Processes and Mechanisms
- Microbial Community Ecology and Physiology
- Gut microbiota and health
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Bone health and treatments
- Immune Cell Function and Interaction
- HIV/AIDS drug development and treatment
- Body Composition Measurement Techniques
- Virus-based gene therapy research
- T-cell and Retrovirus Studies
- Inflammatory mediators and NSAID effects
- Glycosylation and Glycoproteins Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Liver Disease Diagnosis and Treatment
- Reproductive System and Pregnancy
- Bone health and osteoporosis research
- Reproductive tract infections research
Saint Louis University
2015-2024
Washington University in St. Louis
1994-2009
Texas Tech University
2008-2009
Politecnico di Milano
2008
Associazione Sammarinese per la Lotta contro le Leucemie e Emopatie Maligne
2008
Johns Hopkins University
1995-1999
Monsanto (United States)
1999
Johns Hopkins Medicine
1997-1999
Cold Spring Harbor Laboratory
1992-1994
Stony Brook University
1992
A predictive rule for protein folding is presented that involves two recurrent glycine-based motifs cap the carboxyl termini of α helices. In proteins, helices terminated in glycine residues were found predominantly one these motifs. These structures had a characteristic pattern polar and apolar residues. Visual inspection known helical sequences was sufficient to distinguish from each other internal glycines fail terminate motifs—in which local sequence selects between available...
Cyanobacteria are photosynthetic organisms and the only prokaryotes known to have a circadian lifestyle. Unicellular diazotrophic cyanobacteria such as Cyanothece sp. ATCC 51142 produce oxygen can also fix atmospheric nitrogen, process exquisitely sensitive oxygen. To accommodate antagonistic processes, intracellular environment of oscillates between aerobic anaerobic conditions during day-night cycle. This is accomplished by temporal separation two processes: photosynthesis day nitrogen...
Unicellular cyanobacteria have recently been recognized for their contributions to nitrogen fixation in marine environments, a function previously thought be filled mainly by filamentous such as Trichodesmium . To begin systems level analysis of the physiology unicellular N 2 -fixing microbes, we sequenced completion genome Cyanothece sp. ATCC 51142, first organism. 51142 performs oxygenic photosynthesis and fixation, separating these two incompatible processes temporally within same cell,...
<h3>Importance</h3> Chronic rhinosinusitis (CRS) is the persistent inflammation of sinus and nasal passages lasting over 3 months. The etiology CRS not well understood. <h3>Objective</h3> To obtain insights into disease process, we contrasted microbiome immune response from patients with healthy controls. <h3>Design, Setting, Participants</h3> A case vs control design was used. Samples were collected in operating room an institutional hospital or clinic. Thirty 12 controls undergoing surgery...
Nucleos(t)ide analog therapy blocks DNA synthesis by the hepatitis B virus (HBV) reverse transcriptase and can control infection, but treatment is life-long has high costs unpredictable long-term side effects. The profound suppression of HBV nucleos(t)ide analogs their ability to cure some patients indicates that they push brink extinction. Consequently, more could be cured suppressing replication further using a new drug in combination with analogs. ribonuclease H (RNAseH) logical target...
Abstract Cyanobacteria are key contributors to global photosynthetic productivity, and iron availability is essential for cyanobacterial proliferation. While abundant in the earth's crust, its unique chemical properties render it a limiting factor photoautotrophic growth. As compared other nonphotosynthetic organisms, oxygenic organisms such as cyanobacteria, algae, green plants need large amounts of maintain functional PSI complexes their apparatus. Ferritins bacterioferritins ubiquitously...
ABSTRACT Pegylated alpha interferon and ribavirin therapy for hepatitis C virus (HCV) genotype 1 infection fails half of Caucasian American patients (CA) more often African Americans (AA). The reasons these low response rates are unknown. HCV is highly genetically variable, but it unknown how this variability affects to therapy. To assess effects viral diversity on therapy, the complete pretreatment open reading frame was sequenced using samples from 94 participants in Virahep-C study....
Bone is remodeled throughout the life of an animal by action osteoclasts, which resorb bone, and osteoblasts, form new bone. It has recently been recognized that T cells regulate osteoclasts secreting a number cytokines including type I II IFNs receptor activator NF-kappaB ligand. In this study, we show produce chemokines recruit CD8(+) cells. Using transgenic OT-I mice, found in presence OVA, induced secretion IL-2, IL-6, IFN-gamma as well proliferation activated expressed FoxP3, CTLA4, The...
Abstract Background The adaptation of pathogenic fungi to the host environment via large-scale genomic changes is a poorly characterized phenomenon. Cryptococcus neoformans leading cause fungal meningoencephalitis in HIV/AIDS patients, and we recently discovered clinical strains fungus that are disomic for chromosome 13. Here, examined genome plasticity phenotypes monosomic strains, compared their virulence mouse model cryptococcosis Results In an initial set melanin production was...
ABSTRACT The loss of estrogen (E2) initiates a rapid phase bone leading to osteoporosis in one-half postmenopausal women, but the mechanism is not fully understood. Here, we show for first time how E2 activates low-grade inflammation promote acute catabolic activity ovariectomized (OVX) mice. regulates abundance dendritic cells (DCs) that express IL-7 and IL-15 by inducing Fas ligand (FasL) apoptosis DC. In absence E2, DCs become long-lived, increased IL-15. We find together, alone, induced...
The ubiquitously expressed mammalian POU-domain protein Oct-1 specifically recognizes two classes of cis-acting regulatory elements that bear little sequence similarity, the octamer motif ATGCAAAT and TAATGARAT motif. related pituitary-specific POU Pit-1 also these motifs but, unlike Oct-1, binds preferentially to Yet in our assay, still better than does motif-binding Oct-3. domain is responsible for recognizing diverse sequences through multiple DNA contacts include subdomains, POU-specific...
Abstract Light drives the production of chemical energy and reducing equivalents in photosynthetic organisms required for assimilation essential nutrients. This process also generates strong oxidants reductants that can be damaging to cellular processes, especially during absorption excess excitation energy. Cyanobacteria, like other oxygenic organisms, respond increases energy, such as exposure cells high light (HL) by reduction antenna size photosystem content. However, mechanism how...
Hepatitis C virus (HCV) is a common RNA that causes hepatitis and liver cancer. Infection treated with IFN-α ribavirin, but this expensive physically demanding therapy fails in half of patients. The genomic sequences independent HCV isolates differ by approximately 10%, the effects variation on response to are unknown. To address question, we analyzed amino acid covariance within full viral coding region pretherapy from 94 participants Viral Resistance Antiviral Therapy Chronic (Virahep-C)...
Photosynthetic organisms experience changes in light quantity and quality their natural habitat. In response to quality, these redistribute excitation energy adjust photosystem stoichiometry maximize the utilization of available energy. However, other cellular processes is mostly unknown. Here, we report a systematic investigation into adaptation Synechocystis species PCC 6803 that preferentially excites either II or I. We find preferential I induces massive reprogramming transcriptome. The...
Osteoclasts are the body's sole bone resorbing cells. Cytokines produced by pro-inflammatory effector T-cells (T(EFF)) increase resorption osteoclasts. Prolonged exposure to T(EFF) cytokines leads erosion diseases such as osteoporosis and rheumatoid arthritis. The crosstalk between osteoclasts has been termed osteoimmunology. We have previously shown that under non-inflammatory conditions, murine can recruit naïve CD8 activate these induce CD25 FoxP3 (Tc(REG)). activation of also induced...
The ability of the opportunistic fungal pathogen Cryptococcus neoformans to resist oxidative stress is one its most important virulence related traits. To cope with deleterious effect cellular damage caused by burst inside macrophages, C. has developed multilayered redundant molecular responses neutralize stress, repair and eventually grow hostile environment phagosome. We used microarray analysis cells treated hydrogen peroxide (H2O2) at multiple time points in a nutrient defined medium...
Abstract Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer worldwide, tobacco one of factors for HNSCC oral cavity. We have previously observed that bitter melon (Momordica charantia) extract (BME) exerts antiproliferative activity against several cancers including HNSCC. In this study, we investigated preventive role BME in 4-nitroquinoline 1-oxide (4-NQO) carcinogen-induced feeding significantly reduced incidence 4-NQO–induced a mouse model. Histologic analysis...
Pegylated interferon plus ribavirin therapy for hepatitis C virus (HCV) fails in approximately half of genotype 1 patients. Treatment failure occurs either by nonresponse (minimal declines viral titer) or relapse (robust initial responses followed rebounds titers during after therapy). HCV is highly variable genetically. To determine if genetic differences contribute to the difference between response and relapse, we examined inter-patient diversity mutation pattern full open reading frame...