A5006628828- Marine and coastal ecosystems
- Marine Toxins and Detection Methods
- Microbial Community Ecology and Physiology
- Protist diversity and phylogeny
- Diatoms and Algae Research
- Aquatic Ecosystems and Phytoplankton Dynamics
- Algal biology and biofuel production
- Bacteriophages and microbial interactions
- Geotechnical Engineering and Underground Structures
- Marine and coastal plant biology
- Geotechnical Engineering and Analysis
- Marine Biology and Ecology Research
- Robot Manipulation and Learning
- Wastewater Treatment and Nitrogen Removal
- Environmental DNA in Biodiversity Studies
- Robotic Mechanisms and Dynamics
- Environmental Toxicology and Ecotoxicology
- Coastal wetland ecosystem dynamics
- Landslides and related hazards
- Metabolism and Genetic Disorders
- Microscopic Colitis
- Aquaculture disease management and microbiota
- Soil and Environmental Studies
- Geotechnical Engineering and Soil Stabilization
- Physiological and biochemical adaptations
Kōchi University
2015-2025
University of Tokyo Hospital
2021-2023
Ehime University
2004-2022
Aquatic Systems (United States)
2011
Japan Fisheries Research and Education Agency
2006-2007
Toyota Memorial Hospital
2004-2005
National Defense Academy of Japan
1985-2004
Nagoya University
1999-2000
Tokyo Institute of Technology
1992
The marine epiphytic dinoflagellate genus Gambierdiscus produce toxins that cause ciguatera fish poisoning (CFP): one of the most significant seafood-borne illnesses associated with consumption worldwide. So far, occurrences CFP incidents in Japan have been mainly reported subtropical areas. A previous phylogeographic study Japanese revealed existence two distinct phylotypes: sp. type 1 from and 2 temperate However, details genetic diversity distribution for are still unclear, because a...
Background A dinoflagellate genus Ostreopsis is known as a potential producer of Palytoxin derivatives. the most potent non-proteinaceous compound reported so far. There has been growing number reports on palytoxin-like poisonings in southern areas Japan; however, distribution not investigated Morphological plasticity makes reliable microscopic identification difficult employment molecular tools was desirable. Methods/Principal Finding In total 223 clones were examined from samples mainly...
A new epiphytic dinoflagellate is described, G ambierdiscus scabrosus sp. nov., from tidal pools and rocky shores along the coastal areas of Japan. Cells are 63.2 ± 5.7 μm in depth, 58.2 width, 37.3 3.5 length. The plate formula . Po, 4′, 0a, 6′′, 6c, ?s, 5′′′, 0p, 2′′′′. Morphologically, resembles belizeanus as follows: anterioposteriorly compressed cell shape, narrow 2′′′′ plate, areolated surface. Despite this similarity, cells can be distinguishable by presence asymmetric shaped 3′′...
The present study aimed to isolate and characterize a marine bacterium capable of passing through 0.1-μm pore-sized filter (0.1-μm filter). Sediment suspension samples were filtered filters, inoculated into sterile media, incubated. Isolated SspURN76 belonged Saccharospirillum, according 16S rRNA gene sequencing, showed very slender shape. minimum cell size was 0.09×3.2 μm. These morphological features likely responsible for its passage filters. Based on the results obtained herein,...
SUMMARY A high efficiency transformation system was established for the pennate diatom Phaeodactylum tricornutum Bohlin using a plasmid containing fucoxanthin chlorophyll a/c binding protein ( fcp ) promoter/terminator and nitrate reductase NR that are derived from Cylindrotheca fusiformis . The contains zeocin resistance gene ble with promoter enhanced green fluorescent egfp introduced into P. microparticle bombardment. Transformants (650 ± 58 per 10 8 cells) were obtained. yield of...
SUMMARY A nuclear transformation system for the centric diatom Chaetoceros sp. has been established using two plasmids pTpfcp/nat and pTpNR/green fluorescent protein (GFP) that had used Thalassiosira pseudonana transformation. These contain nourseothricin resistance gene ( nat ) with fucoxanthin chlorophyll a /c binding fcp promoter/terminator from T. enhanced green egfp ), nitrate reductase NR , respectively. Transformants were recovered in presence of antibiotic nourseothricin. One to four...
Viruses are considered key players in phytoplankton population control oceans. However, mechanisms that viral gene expression prominent microalgae such as diatoms remain largely unknown. In this study, potential promoter regions isolated from several marine diatom-infecting viruses (DIVs) were linked to the egfp reporter and transformed into Pennales diatom Phaeodactylum tricornutum. We analysed their activity cells grown under different conditions. Compared endogenous promoters, novel DIV...
The dinoflagellate Karenia mikimotoi forms blooms in the coastal waters of temperate regions and occasionally causes massive fish invertebrate mortality. This study aimed to elucidate toxic effect K. on marine organisms by using genomics approach; RNA-sequence libraries were constructed, data analyzed identify toxin-related genes. Next-generation sequencing produced 153,406 transcript contigs from axenic culture mikimotoi. BLASTX analysis against all assembled revealed that 208 polyketide...
Monitoring of harmful algal bloom (HAB) species in coastal waters is important for assessment environmental impacts associated with HABs. Co-occurrence multiple cryptic such as toxic dinoflagellate Ostreopsis make reliable microscopic identification difficult, so the employment molecular tools often necessary. Here we developed new qPCR method by which cells can be enumerated based on actual gene number target species. The assay targets LSU rDNA spp. from Japan. First, constructed standard...
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 73:171-183 (2014) - DOI: https://doi.org/10.3354/ame01713 Temperature alters algicidal activity of DNA and RNA viruses infecting Chaetoceros tenuissimus Y. Tomaru1,*, K. Kimura1,2, H. Yamaguchi3 1National Research Institute Fisheries Environment Inland Sea, Agency, 2-17-5 Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan...
:Species of the benthic dinoflagellate genus Gambierdiscus are putative producer toxins causing ciguatera fish poisoning (CFP) in tropical and subtropical areas. Although CFP events distribution G. caribaeus have been reported Thailand, little is known about conditions that enhance growth Thailand. Hence, this study was aimed at determining effects temperature salinity their interactions on Thai caribaeus. The strain tested showed highest cell yield IMK/2 medium. Based rate (μ), grew range...
This paper presents a randomized manipulation planner for multi-fingered hand by switching contact modes. Manipulation planning such system should consider changing kinematics and dynamics of the according to We derive conditions that would be satisfied in planning, based on kinematical properties system. The allow assign more modes than those corresponding relative motion points restricted constraint forces. Consequently, this enables dextrous manipulation. In addition, give restrictions...
Summary Benthic dinoflagellates of the genus O streopsis produce palytoxin ( PTX )‐like compounds. The worldwide distributed ovata / . cf. is potentially responsible for outbreaks human health problems around coasts tropical, subtropical, and temperate regions. present study examined growth responses an strain s0662 collected from coastal waters J apan with 35 different combinations temperature (15–35° C ) salinity (20–40) discusses bloom dynamics organism in Japanese environments. tolerated...