A5079576536- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Reproductive biology and impacts on aquatic species
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Aquaculture Nutrition and Growth
- Aquaculture disease management and microbiota
- Fish Ecology and Management Studies
- Cancer, Hypoxia, and Metabolism
- Physiological and biochemical adaptations
- Fish biology, ecology, and behavior
- Muscle Physiology and Disorders
- RNA Interference and Gene Delivery
- High Altitude and Hypoxia
- RNA and protein synthesis mechanisms
- Sperm and Testicular Function
- interferon and immune responses
Auburn University
2020-2024
Arkansas Agricultural Experiment Station
2021
CRISPR/Cas9-based gene knockout in animal cells, particularly teleosts, has proven to be very efficient with regards mutation rates, but the precise insertion of exogenous DNA or knock-in via homology-directed repair (HDR) pathway seldom been achieved outside model organisms. Here, we succeeded integrating high efficiency an alligator cathelicidin into a targeted non-coding region channel catfish (Ictalurus punctatus) chromosome 1 using two different donor templates (synthesized linear dsDNA...
Abstract Despite catfish being the dominant freshwater aquaculture product in United States, texture and sensory evaluation are understudied compared with other species, very few studies have been conducted to evaluate these traits catfish. Texture, sensory, carcass yield, flavor, visceral fat deposition, gonadal development, fillet color analyses were on four size classes, small (<0.68 kg), medium (0.68–0.92 large (0.93–1.75 extra‐large (>1.75 for channel ( n = 456) Ictalurus...
Abstract Xenogenesis is an innovative tool for hybrid catfish (female Channel Catfish Ictalurus punctatus × male Blue I. furcatus ) seed production, accomplished by transplanting undifferentiated germ cells derived from a donor diploid fish into sterile recipient, which then enables recipient to produce donor‐derived gametes. There potential collect mature during certain times of the year depending upon seasonal temperature and serum sex steroid hormonal fluctuations. The objective this...
CRISPR/Cas9-mediated knock-in (KI) has a wide application in gene therapy, function study, and transgenic breeding programs. Unlike which requires accurate KI to correct mutation, programs can accept robust as long integration does not interrupt normal functions result any negative pleiotropic effects. High efficiency is required reduce the cost shorten period, especially transferring multiple foreign genes single individual. To elevate efficacy achieve KIs simultaneously, we introduced new...
Xenogenesis has been recognized as a prospective method for producing channel catfish, Ictalurus punctatus ♀ × blue I . furcatus ♂ hybrids. The xenogenesis procedure can be achieved by transplanting undifferentiated stem cells derived from donor fish into sterile recipient. hybrid catfish embryo production accomplished using triploid surrogate. However, having surrogate species with shorter maturation period, like white ( Ameiurus catus ), would result in reduced feed costs, labor and...
Tra catfish ( Pangasianodon hypophthalmus ), also known as striped catfish, is a facultative air-breather that uses its swim bladder an air-breathing organ (ABO). A related species in the same order (Siluriformes), channel Ictalurus punctatus does not possess ABO and thus cannot breathe air. serve great comparative models for investigating possible genetic underpinnings of aquatic to land transitions, well understanding genes are crucial development function tra catfish. In this study,...
Abstract Channel catfish, Ictalurus punctatus , have limited ability to synthesize Ω-3 fatty acids due a lack of elongases and desaturases. The ccβA-msElovl2 transgene containing masu salmon, Oncorhynchus masou elongase gene driven by the common carp, Cyprinus carpio beta-actin promoter was inserted into channel catfish melanocortin-4 receptor ( mc4r ) site using two-hit two-oligo with plasmid (2H2OP) method. best performing sgRNA resulted in knockout mutation rate 92% 69%...