A5090057967- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Aquaculture Nutrition and Growth
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive biology and impacts on aquatic species
- Marine Bivalve and Aquaculture Studies
- Marine Biology and Environmental Chemistry
- Aquaculture disease management and microbiota
- Neurobiology and Insect Physiology Research
- Marine Ecology and Invasive Species
- Parasite Biology and Host Interactions
- Genetic and phenotypic traits in livestock
- Cephalopods and Marine Biology
- Environmental Toxicology and Ecotoxicology
- Genetic Mapping and Diversity in Plants and Animals
- Genomics and Phylogenetic Studies
- interferon and immune responses
- RNA Research and Splicing
- Marine Biology and Ecology Research
- Developmental Biology and Gene Regulation
- Physiological and biochemical adaptations
- Fish Ecology and Management Studies
- MicroRNA in disease regulation
- Genetic diversity and population structure
- Prion Diseases and Protein Misfolding
Auburn University
2014-2024
Ocean University of China
2014-2024
Northeast Agricultural University
2024
Institute of Pomology
2015
Chinese Academy of Inspection and Quarantine
2008-2009
Abstract Catfish represent 12% of teleost or 6.3% all vertebrate species, and are enormous economic value. Here we report a high-quality reference genome sequence channel catfish ( Ictalurus punctatus ), the major aquaculture species in US. The was validated by genetic mapping 54,000 SNPs, annotated with 26,661 predicted protein-coding genes. Through comparative analysis genomes transcriptomes scaled scaleless fish scale regeneration experiments, address genomic basis for most striking...
Construction of genetic linkage map is essential for and genomic studies. Recent advances in sequencing genotyping technologies made it possible to generate high-density high-resolution maps, especially the organisms lacking extensive resources. In present work, we constructed a channel catfish with three large resource families genotyped using 250K single-nucleotide polymorphism (SNP) array. A total 54,342 SNPs were placed on map, which our knowledge had highest marker density among...
CRISPR/Cas9-based gene knockout in animal cells, particularly teleosts, has proven to be very efficient with regards mutation rates, but the precise insertion of exogenous DNA or knock-in via homology-directed repair (HDR) pathway seldom been achieved outside model organisms. Here, we succeeded integrating high efficiency an alligator cathelicidin into a targeted non-coding region channel catfish (Ictalurus punctatus) chromosome 1 using two different donor templates (synthesized linear dsDNA...
Summary Catfish is the leading aquaculture species in United States. The interspecific hybrid catfish produced by mating female channel with male blue outperform both of their parent a number traits. However, mass production hybrids has been difficult because reproductive isolation. Investigations genome structure and organization provide insights into genetic basis for maintenance divergence face gene flow, thereby helping develop strategies introgression efficient aquaculture. In this...
As an important transcription factor, SOX2 involves in embryogenesis, maintenance of stem cells and proliferation primordial germ cell (PGC). However, little was known about its function mature gonads. Herein, we investigated the gene profiles testis scallop, Chlamys farreri. The level C. farreri (Cf-SOX2) mRNA increased gradually along with gonadal development reached peak at stage, located all cells, including spermatogonia, spermatocytes, spermatids spermatozoa. Knockdown Cf-SOX2 using...
The intertidal zone is a transitional area of the land-sea continuum, in which physical and chemical properties vary during tidal cycle highly toxic sulfides are rich sediments due to dynamic regimes. As typical species thriving this habitat,
Mollusca is the second largest animal phylum and represents one of most evolutionarily successful groups. Mulinia lateralis , a small bivalve, promising model organism to facilitate studies mollusc development. However, because lack published genomic transcriptomic resources, integrated research on formation larval shells in this species, which representative developmental process molluscs great importance for larva survival, hindered. In study, blastula, gastrula, trochophore larva,...
Abstract Vitellogenin ( VTG ), the precursor of yolk protein, plays an important role during vitellogenesis and oogenesis. However, little is known about vitellogenin gene in marine bivalves. We cloned full‐length cDNA C hlamys farreri Cf‐vtg ) using a homologous cloning strategy rapid amplification ends. The consisted 7604 nucleotides with open reading frame encoding 2296 amino acid residues. deduced sequence shared high similarity other species, particularly N‐terminal region. analyzed...
Transcription activator-like effector nuclease (TALEN) plasmids targeting the channel catfish gonadotropin-releasing hormone (cfGnRH) gene were delivered into fertilized eggs with double electroporation to sterilize (Ictalurus punctatus). Targeted cfGnRH fish sequenced and base deletion, substitution, insertion detected. The mutagenesis was achieved in 52.9% of P1 fish. mutants (individuals human-induced sequence changes at locus) had lower spawning rates (20.0−50.0%) when there no therapy...
In many bilaterians, Hox genes are generally clustered along the chromosomes and expressed in spatial temporal order. vertebrates, expression of follows a whole-cluster spatio-temporal collinearity (WSTC) pattern, whereas some invertebrates exhibits subcluster-level pattern. diversity patterns cause differences gene remain poorly understood. Here, we investigate genomic organization pattern echiuran worm Urechis unicinctus (Annelida, Echiura). has split cluster with four subclusters divided...
The larval segment formation and secondary loss in echiurans is a special phenomenon, which considered to be one of the important characteristics evolutionary relationship between Echiura Annelida. To better understand molecular mechanism this we revealed transcriptome profile echiuran worm Urechis unicinctus using RNA-Seq technology. Twelve cDNA libraries U. larvae, late-trochophore (LT), early-segmentation larva (ES), segmentation (SL), worm-shaped (WL) were constructed. Totally 243,381...
Piwi (P-element induced wimpy testis) is an important gene involved in stem cell maintenance and gametogenesis vertebrates. However, most invertebrates, especially mollusks, the function of during remains largely unclear. To further understand gametogenesis, full-length cDNA Piwi1 from scallop Chlamys farreri (Cf-Piwi1) was characterized, which consisted a 2,637 bp open reading frame encoding 878-amino acid protein. Cf-Piwi1 mRNA mainly localized spermatogonia, spermatocytes, oogonia,...