A5103254981- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Hepatitis B Virus Studies
- Cancer Immunotherapy and Biomarkers
- vaccines and immunoinformatics approaches
- Viral gastroenteritis research and epidemiology
- IL-33, ST2, and ILC Pathways
- Plant biochemistry and biosynthesis
- Psoriasis: Treatment and Pathogenesis
- Cardiac and Coronary Surgery Techniques
- Monoclonal and Polyclonal Antibodies Research
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA Research and Splicing
- CAR-T cell therapy research
- Cancer Cells and Metastasis
- Insect Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Immune cells in cancer
- Cardiac Ischemia and Reperfusion
- Nasal Surgery and Airway Studies
- RNA modifications and cancer
Army Medical University
2013-2025
Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine
2025
Beijing Forestry University
2024
Hebei Agricultural University
2023-2024
Qiqihar University
2023
Second Xiangya Hospital of Central South University
2014-2023
Central South University
2008-2023
Second Affiliated Hospital of Xi'an Jiaotong University
2019-2023
Institute of Pomology
2012-2022
Chinese Academy of Agricultural Sciences
2017-2022
Abstract A complete and accurate genome sequence provides a fundamental tool for functional genomics DNA-informed breeding. Here, we assemble high-quality (contig N50 of 6.99 Mb) the apple anther-derived homozygous line HFTH1, including 22 telomere sequences, using combination PacBio single-molecule real-time (SMRT) sequencing, chromosome conformation capture (Hi-C) optical mapping. In comparison to Golden Delicious reference genome, identify 18,047 deletions, 12,101 insertions 14 large...
Anthocyanin and proanthocyanidin (PA) are important secondary metabolites beneficial to human health. Their biosynthesis is induced by jasmonate (JA) treatment regulated MYB transcription factors (TFs). However, which how TFs regulate this process largely unknown in apple. In study, MdMYB9 MdMYB11 were methyl (MeJA) functionally characterized. Overexpression of or promoted not only anthocyanin but also PA accumulation apple calluses, the was further enhanced MeJA. Subsequently, yeast...
Abstract Catfish represent 12% of teleost or 6.3% all vertebrate species, and are enormous economic value. Here we report a high-quality reference genome sequence channel catfish ( Ictalurus punctatus ), the major aquaculture species in US. The was validated by genetic mapping 54,000 SNPs, annotated with 26,661 predicted protein-coding genes. Through comparative analysis genomes transcriptomes scaled scaleless fish scale regeneration experiments, address genomic basis for most striking...
CD4+CD25+Foxp3+ regulatory T cells (Tregs) can inhibit cytotoxic responses. Though several studies have analyzed Treg frequency in the peripheral blood mononuclear (PBMCs) of pancreatic ductal adenocarcinoma (PDA) patients using flow cytometry (FCM), few examined how intratumoral Tregs might contribute to immunosuppression tumor microenvironment. Thus, potential role PDA remains be elucidated. In this study, we found that percentages Tregs, CD4+ and CD8+ were all increased significantly...
T cell activation is a well-established model for studying cellular responses to exogenous stimulation. Using strand-specific RNA-seq, we observed that intron retention prevalent in polyadenylated transcripts resting CD4(+) cells and significantly reduced upon activation. Several lines of evidence suggest intron-retained are less stable than fully spliced transcripts. Strikingly, the decrease (IR) levels correlate with increase steady-state mRNA levels. Further, majority genes upregulated...
Summary Allele‐specific expression (ASE) can lead to phenotypic diversity and evolution. However, the mechanisms regulating ASE are not well understood, particularly in woody perennial plants. In this study, we investigated genes apple cultivar ‘Royal Gala’ (RG). A high quality chromosome‐level genome was assembled using a homozygous tetra‐haploid RG plant, derived from anther cultures. Using RNA‐sequencing (RNA‐seq) data flower fruit tissues, identified 2091 genes. Compared with haploid of...
IL-23 regulates myriad processes in the innate and adaptive immune systems, is a critical mediator of proinflammatory effects exerted by Th17 cells many diseases. In this study, we investigated whether how hepatitis B virus (HBV) causes liver damage directly through signaling pathway. biopsied tissues from HBV-infected patients, expression both IL-23R was remarkably elevated. vivo observations also indicated that main sources were myeloid dendritic (mDCs) macrophages. Analysis vitro...
Temporary COVID-19 specialty hospitals proved to be useful in the control of an infectious crisis a large epicenter, and hopefully this approach provides blueprint for management future epidemics or pandemics.
Thymus is the main immune organ which responsible for production of self-tolerant and functional T cells, but it shrinks rapidly with age after birth. Although studies have researched thymus development involution in mouse, critical regulators that arise human remain unclear. We collected public single-cell transcriptomic sequencing (scRNA-seq) datasets containing 350,678 cells from 36 samples, integrated them as a cell atlas thymus. Clinical samples were experiments performed validation....
Gene expression in apple roots response to various stress conditions is a less-explored research subject. Reliable reference genes for normalizing quantitative gene data have not been carefully investigated. In this study, the suitability of set 15 were evaluated their potential use as reliable genes. These selected based on low variance root tissues from recent RNA-seq set, and few previously reported other tissue types. Four methods, Delta Ct, geNorm, NormFinder BestKeeper, used evaluate...
Cancer is typically considered as a genetic and epigenetic disease. Although numerous studies have indicated that an aberrant structure, function, or expression level of enzymes contribute to many tumor types, precisely how the mechanisms are involved in hepatitis B virus (HBV)-induced hepatocellular carcinoma (HCC) remains unknown.In this study, we found WD repeat domain 5 protein (WDR5)-a core subunit histone H3 lysine 4 methyltransferase complexes, which catalyze generation trimethylation...
Abiotic stress reduces plant yield and quality. WRKY transcription factors play key roles in abiotic responses plants, but the molecular mechanisms by which mediate to drought osmotic stresses apple (Malus × domestica Borkh.) remain unclear. Here, we functionally characterized Group III gene MdWRKY115. qRT-PCR analysis showed that MdWRKY115 expression was up-regulated stresses. GUS activity revealed promoter of enhanced under stress. Subcellular localization transactivation assays indicated...
Reproducing the pathophysiology of human multiple sclerosis (MS) in animal models is critical to identifying mechanisms triggering demyelination and developing early intervention strategies. Here, we aimed model overactivated Wnt (wingless-related integration site) signaling previously shown postmortem brain tissues patients with MS by inducing experimental autoimmune encephalomyelitis (EAE) Pdgfra CreER ;Apc fl/fl Olig2 Cre mice. These mice have oligodendrocyte precursor cells (OPCs)...
Background Alzheimer disease (AD) is a neurodegenerative related to aging. At present, its pathological mechanisms remain unclear. Family members of the renin-angiotensin system (RAS) play role in neuronal plasticity, as well formation learning and memory. In this study, we explore effects altered angiotensin-converting enzyme (ACE), investigate possible perindopril, an ACE inhibitor, on brain structure function rat model AD, that plays AD. Methods Sixty Sprague-Dawley rats were selected...
Estrogen deficiency in postmenopausal women is a major cause of bone loss, resulting osteopenia, osteoporosis, and high risk for fracture. Connexin 43 (Cx43) hemichannels (HCs) osteocytes play an important role osteocyte viability, formation, remodeling. We showed here that estrogen reduced Cx43 expression HC function. To determine if functional HCs protect loss during deficiency, we adopted ovariectomy model wild-type (WT) two transgenic mice: R76W (dominant-negative mutant inhibiting only...
Aging | doi:10.18632/aging.103535. Zhiguo Zhou, Min Zhang, Yali Wang, Fang Zheng, Yaxiong Huang, Kang Qizhi Yu, Chunlin Cai, Dong Chen, Yi Tian, Jianhua Lei, Xinqiang Xiao, Erik De Clercq, Guangdi Li, Yuanlin Xie, Guozhong Gong
Regulatory T cells (Treg) contribute to the crucial immunological processes of self-tolerance and immune homeostasis. Genomic mechanisms that regulate cell fate decisions leading Treg or conventional (Tconv) lineages those underlying function remain be fully elucidated, especially at histone modification level. We generated high-resolution genome-wide distribution maps monomethylated H3 lysine 4 (H3K4me1) trimethylated H3K4 (H3K4me3) in human CD4+CD25+FOXP3+ Tregs CD4+CD25+FOXP3− activated...