A5100742193- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Pharmacological Receptor Mechanisms and Effects
- Protein Interaction Studies and Fluorescence Analysis
- Parasites and Host Interactions
- MicroRNA in disease regulation
- Drug Transport and Resistance Mechanisms
- DNA and Nucleic Acid Chemistry
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Cancer Immunotherapy and Biomarkers
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Surfactants and Colloidal Systems
- IL-33, ST2, and ILC Pathways
- Research on Leishmaniasis Studies
- Chemical Synthesis and Analysis
- Cytokine Signaling Pathways and Interactions
- Ferroptosis and cancer prognosis
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Synthesis and Reactivity of Heterocycles
- Veterinary Pharmacology and Anesthesia
Soochow University
2024
Second Affiliated Hospital of Soochow University
2024
Nanjing Medical University
2021-2024
Henan Polytechnic University
2022
Jiyang College of Zhejiang A&F University
2022
Stomatology Hospital
2015-2021
Zhejiang University
2015-2021
Shanghai Institute of Materia Medica
2004-2021
Chinese Academy of Sciences
2004-2021
Xiangya Hospital Central South University
2020-2021
CD4+CD25+Foxp3+ regulatory T cells (Tregs) can inhibit cytotoxic responses. Though several studies have analyzed Treg frequency in the peripheral blood mononuclear (PBMCs) of pancreatic ductal adenocarcinoma (PDA) patients using flow cytometry (FCM), few examined how intratumoral Tregs might contribute to immunosuppression tumor microenvironment. Thus, potential role PDA remains be elucidated. In this study, we found that percentages Tregs, CD4+ and CD8+ were all increased significantly...
Pancreatic cancer remains one of the most malignant tumors with a poor prognosis. Despite advances in diagnosis and treatment, no reliable biomarkers are available for clinical practice. Circular RNAs (circRNAs) novel class endogenous non‑coding RNA, which abundant, stable conserved, serve crucial roles disease, particularly cancer. The purpose present study was to investigate expression profile circRNAs 20 pancreatic tissues corresponding paracancerous using arraystar human circRNA array...
The effect of a naphthalimide pharmacophore coupled with diverse substituents on the interaction between naphthalimide-polyamine conjugates 1–4 and bovine serum albumin (BSA) was studied by UV absorption, fluorescence circular dichroism (CD) spectroscopy under physiological conditions (pH = 7.4). observed spectral quenching BSA compounds indicated that they could bind to BSA. Furthermore, caloric fluorescent tests revealed mechanisms 1–3 were basically static type, but compound 4 closer...
// Yu You 1 , Jia-Xin Tan Hai-Su Dai Hao-Wei Chen Xue-Jun Xu Ai-Gang Yang Yu-Jun Zhang Lian-Hua Bai Ping Bie Department of Hepatobiliary Surgery Institute, South Western Hospital, Third Military Medical University, Chongqing 400038, China Correspondence to: Bie, email: bieping@medmail.com.cn Bai, 870426131@qq.com Keywords: hepatocellular carcinoma, hepatic stellate cells, galectin-1, miRNA-22 Received: April 14, 2016 Accepted: July 10, Published: August 1, 2016...
The objective of this study was to investigate the expression and clinical relevance interleukin 22 (IL-22) IL-22-producing CD4 T cells (IL-22CD4 cells) in pancreatic cancer (PC) tissues.Interleukin protein levels PC tissues were measured by Western blot analysis immunohistochemistry. frequencies IL-22CD4 tumors peripheral blood from patients control subjects analyzed flow cytometry. association between IL-22 phosphorylation STAT-3 investigated vitro model.Interleukin more highly expressed...
Hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1) is a key enzyme in the mevalonate pathway of cholesterol synthesis. Dysregulation HMGCS1 expression common occurrence many solid tumors. It was also found to be overexpressed newly diagnosed (ND) and relapsed/refractory (RR) acute myeloid leukemia (AML) patients. Previous study proved that could induce drug-resistance AML cells. However, underlying mechanism how contributed chemoresistance remains elusive. Here, we confirmed inhibitor...
ATPM [(-)-3-amino-thiazolo[5,4-<i>b</i>]-<i>N</i>-cyclopropylmethylmorphinan hydrochloride] was found to have mixed κ- and μ-opioid activity identified act as a full κ-agonist partial μ-agonist by in vitro binding assays. The present study undertaken characterize its vivo effects on morphine antinociceptive tolerance mice heroin self-administration rats. demonstrated yield more potent than (-)U50,488H...
A novel method, named as the plasma protein-interaction QSAR analysis (PPI-QSAR) was used to construct models for human protein binding. The intra-molecular descriptors of drugs and inter-molecular interaction resulted from docking simulation between drug molecules serum albumin were included independent variables in this method. structure-based silico model a data set 65 antibiotic constructed by multiple linear regression method validated residual analysis, normal Probability-Probability...
The extremely high proliferation rate of tumor cells contributes to pancreatic cancer (PC) progression. Runt-related transcription factor 1(RUNX1), a key in hematopoiesis that was correlated with However, the role RUNX1 PC still unclear. We found significantly upregulated tissues and its expression negatively associated prognosis patients multicenter analysis according immunohistochemical (IHC). downregulation resulted reduced cell rate, which consistent vivo subcutaneous formation assay...
Regulatory T cells (Tregs) can alleviate the development of autoimmune and inflammatory diseases, thereby proposing their role as a new therapeutic strategy. Parasitic helminths have co-evolved with hosts to generate immunological privilege immune tolerance through inducing Tregs. Thus, constructing "Tregs-induction"-based discovery pipeline from parasitic helminth is promising strategy control diseases.The gel filtration chromatography reverse-phase high-performance liquid (RP-HPLC) were...
Distinct opioid receptor agonists have been proved to induce differential patterns of ERK activation, but the underlying mechanisms remain unclear. Here, we report that Ser363 in δ-opioid (δOR) determines different abilities δOR DPDPE and TIPP activate by G-protein- or β-arrestin-dependent pathways. Although both activated ERK1/2, they showed temporal, spatial desensitization activation. We show employed G protein as primary mediator cascade an Src-dependent manner, whereas mainly adopted a...
Purpose: Dendritic cells (DCs) play critical roles in promoting innate and adaptive immunity microbial infection. Functional impairment of DCs may mediate the suppression viral-specific T-cell immune response chronic hepatitis B (CHB) patients. Osteopontin (OPN) is involved several liver diseases infectious diseases. However, whether OPN affects DC function virus (HBV) infection unknown. Methods: Twenty CHB patients 20 healthy volunteers were recruited. secreted by was compared. Peripheral...
Effective and safe analgesics represent an unmet medical need for the treatment of acute chronic pain. A series N-cyclopropylmethyl-7α-phenyl-6,14-endoethanotetrahydronorthebaines were designed, synthesized, assayed, leading to discovery a benzylamine derivative (compound 4, SLL-039) as highly selective potent κ opioid agonist (κ, Ki = 0.47 nM, κ/μ 682, κ/δ 283), which was confirmed by functional assays in vitro antinociceptive vivo. The vivo effect could be blocked pretreatment with...
The classic "message-address" concept was proposed to address the binding of endogenous peptides opioid receptors and later successfully applied in discovery first nonpeptide δ receptor (DOR) antagonist naltrindole. By revisiting this concept, based on structure tramadol, we designed a series novel compounds that act as highly potent selective agonists DOR among which (-)-6j showed highest affinity (K i = 2.7 nM), best agonistic activity (EC50 2.6 selectivity (more than 1000-fold over other...
To develop novel analgesics with no side effects or less than traditional opioids is highly demanded to treat opioid receptor mediated pain and addiction issues. Recently, κ-opioid (KOR) has been established as an attractive target, although its selective agonists could bear heterogeneous pharmacological activities. In this study, we designed synthesized two new series of nepenthone derivatives by inserting a spacer (carbonyl) between 6α,14α-endo-ethenylthebaine the 7α-phenyl substitution...
Background: Glioma is a prevalent disease of the central nervous system with high incidence and mortality rate. Many long noncoding RNAs (lncRNAs) have been determined to be critical regulators glioma oncogenesis. However, function mechanism LINC00963 in not fully elucidated. Methods: The expression level RNA was by qRT-PCR, protein Western blot analysis. A luciferase activity assay conducted verify interaction between miRNA lncRNA or target gene. proliferation, cell cycle distribution,...