A5061443513- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Research on Leishmaniasis Studies
- Helminth infection and control
- Macrophage Migration Inhibitory Factor
- IL-33, ST2, and ILC Pathways
- Helicobacter pylori-related gastroenterology studies
- Tuberculosis Research and Epidemiology
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Hepatitis Viruses Studies and Epidemiology
- Diabetes and associated disorders
- Insect symbiosis and bacterial influences
- Diet, Metabolism, and Disease
- Mosquito-borne diseases and control
- Liver Disease Diagnosis and Treatment
- Tryptophan and brain disorders
- Neuroendocrine regulation and behavior
- Vitamin K Research Studies
- T-cell and B-cell Immunology
- Innovations in Medical Education
- Reproductive System and Pregnancy
- Vitamin D Research Studies
- COVID-19 Impact on Reproduction
- vaccines and immunoinformatics approaches
Nanjing Medical University
2016-2025
Xi'an Technological University
2024
State Key Laboratory of Reproductive Medicine
2022-2024
Shanghai Mental Health Center
2020
Shanghai Jiao Tong University
2020
National Health and Family Planning Commission
2020
Texas Children's Hospital
2013
Baylor College of Medicine
2013
Houston Methodist
2013
Methodist Hospital
2013
Colitis-associated cancer (CAC) is the most serious outcome of inflammatory bowel disease, which has an alteration commensal intestinal microbiota. However, role microbiota on CAC progression not well understood. Fecal transplantation (FMT) was used for treating murine azoxymethane–dextran sodium sulfate (AOM-DSS) model CAC. Composition gut during FMT treatment analyzed. RT-PCR and ELISA were to detect factors, immunofluorescence applied examine phospho-NF-κB p65/p100 Ki67 positive cells in...
Abstract Cervical cancer (CC) is one of the most deadly cancers in women, its current treatments still result poor outcomes and developing novel targets therapeutic strategies are urgently needed. Recent studies have shown that anti-silencing function 1B (ASF1B) might be used as a new proliferation marker for diagnosis prognosis. However, expression ASF1B cervical remain unclear. Here, we induced knockdown overexpression cell lines detected biological behavior changes vitro. Furthermore,...
A number of epidemiological and clinical studies have suggested an inverse association between allergy helminth infection, such as Schistosomiasis. Therefore, we hypothesize that Schistosoma japonicum egg antigens, a type native antigen, can induce production CD4(+) CD25(+) T cells with regulatory activity, modulating airway inflammation inhibiting asthma development. The frequency was determined by flow cytometry for mice treated ovalbumin (OVA), depletion/OVA, schistosome antigens/OVA...
Difficulty in controlling human Schistosoma japonicum infection is partly attributed to the presence of non-human definitive hosts. Water buffaloes are a major reservoir for transmission S. humans China. However, Philippines, reports based on microscopic examination buffalo stool identified low prevalence japonicum, and mathematical models using these data concluded that water not humans. We collected from 81 Macanip, Leyte, assayed by Danish Bilharziasis Laboratory technique, Kato-Katz...
Glioma is one of the most common brain tumors, suggesting importance investigating molecular mechanism gliomas. We studied roles Ribonucleotide Reductase Regulatory Subunit M2 (RRM2) in glioma. Expressions RRM2 are higher glioma tissues evidenced by TCGA data, western blot and immunohistochemistry. negatively correlated with patient's survival. RNA-seq showed that genes involved apoptosis, proliferation, cell adhesion negative regulation signaling were up-regulated upon RNAi-mediated...
Previous studies have indicated that indolepropionic acid (IPA), derived from dietary tryptophan via gut microbiota conversion, is negatively correlated with type 2 diabetes mellitus and systemic low-grade inflammation. However, the effects of IPA administration on obesity, as well underlying mechanisms, remain unclear. In present study, we observed obesity leads to a dramatic reduction in levels both serum colonic mucosa, supplementation exerted beneficial weight, glucose lipid metabolism...
Abstract Background Research is now focused on identification of sensitive and specific diagnostic tests for early schistosomal infection evaluation chemotherapy in field situations China. Results This study compared loop-mediated isothermal amplification (LAMP) with conventional PCR as DNA-based techniques the detection DNA chemotherapy. The results showed that both LAMP assays targeting a 301 base pair (bp) sequence highly repetitive retrotransposon, SjR2, amplified from schistosomes but...
Dendritic cell (DC) vaccines targeting only cancer cells have produced limited antitumor activity in most clinical studies. Targeting cancer-associated fibroblasts (CAFs) addition to may enhance effects, since CAFs, the central component of tumor stroma, directly support growth and contribute immunosuppressive microenvironment. To co-target CAFs we developed a new compound DC vaccine that encodes an A20-specific shRNA function, targets fibroblast activation protein (FAP) expressed antigen...
A Schistosoma mansoni cercarial antigen preparation (cercarial transformation fluid – SmCTF) was evaluated for detection of anti-schistosome antibodies in human sera 4 collaborating laboratories. The performance SmCTF compared with that S. egg antigens (SmSEA) an indirect enzyme-immunoassay (ELISA) assay, the latter being used routinely 3 participating laboratories to diagnose and haematobium infections. In fourth laboratory japonicum (SjSEA) ELISA anti-S. antibodies. all results given by...
Wolbachia has been reported to suppress a variety of pathogen infections in mosquitoes, but the mechanism is undefined. Two possibilities have proposed. One that activates host immune responses, and other one competes with pathogens for limited nutrients.In this study, we compared responses densities two different strains naturally occurring parental artificially created hybrid genetic backgrounds. No significant difference density was found between these hosts. We could activate innate when...
Background: The host-parasite relationship is based on subtle interplay between parasite survival strategies and host defense mechanisms. It well known that helminth infection, which afflicts more than one billion people globally, correlates with a decreased prevalence of obesity. Dissecting the underlying mechanisms can provide new targets for treating obesity from interaction perspective. Methods: C57BL/6 mice received normal or high-fat diet (HFD) without Sjp40 (one main component...
Acetaminophen induced acute liver injury (ALI) has a high incidence and is serious medical problem, but there lack of effective treatment. The enterohepatic axis one the targets recent attention due to its important role in diseases. Disulfiram (DSF) multitarget drug that been proven play variety diseases can affect intestinal flora, whether it alleviate ALI not clear. We utilised bacterial 16S rRNA gene profiling, antimicrobial treatments, faecal microbiota transplantation tests explore DSF...
Recent investigations indicate that schistosome infection is closely associated with aberrant glycolipid metabolism. However, the actual metabolism gene expression, as well possible pathways regulate in schistosome-infected liver, has not been extensively explored. In this study, we evaluated dynamic expression of metabolism-associated genes and proteins livers from mice infected Schistosoma japonicum at indicated time points using real-time PCR immunofluorescence. Then, cultures macrophages...
Abstract Background Dysbiosis of gut microbiota is causally linked to impaired host glucose metabolism. We aimed study effects the new method fecal transplantation, washed transplantation (WMT), on reducing glycemic variability (GV) in unstable diabetes. Methods Fourteen eligible patients received three allogenic WMTs and were followed up at 1 week, month, 3 months. Primary outcomes daily insulin dose, excursions during meal tests, GV indices calculated from continuous monitoring or...
Little is known about the functions of Toll-like receptor 2 (TLR2) and TLR4 in innate/acquired responses to Schistosoma japonicum. Through vivo study, contributions TLR2 host immune during S. japonicum infection were investigated. Early experiments showed higher protein mRNA levels IL-12, IFN-γ IL-4 dermal tissues retroauricular draining lymph nodes respectively TLR2(-/-) mice on day four post-infection opposite changes TLR4(-/-) mice. In acute with for 6 weeks, manifested lower egg burden...
The vaccination of radiation-attenuated Schistosoma japonicum cercariae can induce effective protection in artiodactyl, but the immune events related to protective immunity are not fully understood. To provide a paradigm for human recombinant antigen vaccine, we have undertaken and challenge experiment pigs, which was recognized as an appropriate animal model this type study because their similarity immunology, investigated relative induced by S. cercariae.We found that pigs immunized once...
Cervical cancer is a common gynecologic and frequent cause of death.In this study, we investigated the role MELK (maternal embryonic leucine zipper kinase) in cervical cancer.We found that HPV 18 E6/E7 promoted expression by activating E2F1.MELK knockdown blocked cells growth.Furthermore, used MELK-8A to inhibit kinase activity caused G2/M phase arrest cells.Under treatment inhibitors, Hela formed multipolar spindles eventually underwent apoptosis.We also involved protein translation folding...
Schistosomiasis japonica is a major public health problem in the endemic areas of China, Philippines, and Indonesia. To date, vaccine has not been developed against this disease but immunization with UV-attenuated cercariae can induce high level protective immunity Landrace/Yorkshire/Duroc crossbred pigs. compare efficacy single vaccination multiple vaccinations Schistosoma japonicum cercariae, two groups pigs received either one or three exposures to 10,000 attenuated 400 μw UV.Pigs had...
Toll-like receptor 2 (TLR2) was shown to be an important immune involved in the recognition of schistosome antigens, especially soluble egg antigen (SEA). In mice models with Schistosoma japonicum acute infection, we observed enhanced T cell-mediated responses TLR2 knock out (TLR2−/−) compared B6 mice. chronic infection models, programmed death ligand 1 (PD-L1) and (PD-L2) expression as well gradually increased mice, while only PD-L2 significantly decreased TLR2−/− Meanwhile, Programmed...